Abberior STED

📊 View QC & Maintenance History

Current Status: 🟢 Online

Operational

Instrument ID

scope-1e33f909

Manufacturer

Abberior

Model

STED/RESOLFT

Stand

other

Location

BioCity, 5th floor, BioPhysics room 5086

Purchased

—

Funding

—

Modalities

STED RESOLFT confocal_point

Modules

Easy3D_STED adaptive_optics RESCue_STED

Capabilities

• Notes: Abberior STED. Sophisticated and highly flexible open platform that can be modified, extended, and upgraded. Typical resolution is ~40 nm in XY and ~150 nm in Z using Easy3D STED. Easy3D uses a programmable spatial light modulator (SLM) to create 2D/3D STED phase patterns. Easy3D with adaptive optics enables deep imaging up to 75 µm into cleared tissue with a 100x oil objective. RESCue STED is available for live super-resolution imaging with reduced light dose.

Software Environment

• Abberior Imspector

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  Component: acquisition
  

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Hardware Configuration

💡 Light Sources

• 488 nm

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  Type: laser
  Wavelength: 488 nm

  
  pulsed excitation

• 561 nm

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  Type: laser
  Wavelength: 561 nm

  
  pulsed excitation

• 640 nm

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  Type: laser
  Wavelength: 640 nm

  
  pulsed excitation

• 580 nm

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  Type: laser
  Wavelength: 580 nm

  
  STED depletion (continuous, for 488 nm)

• 775 nm

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  Type: laser
  Wavelength: 775 nm

  
  STED depletion (pulsed, for 561 & 640 nm)

📷 Detectors

• Unknown Photomultiplier Tube

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  Type: PMT

• Unknown Avalanche Photodiode

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  Type: APD
  2 detectors

• Unknown Monochrome CCD camera

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  Type: CCD
  1280x960 pixels

🔬 Objectives

• Plan
  20x_air

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  Mag: 20.0x | NA: 0.4
  Immersion: air
  WD: 1.2 mm
  Correction: plan_apochromat

• UPLSAPO
  60x_water

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  Mag: 60.0x | NA: 1.2
  Immersion: water
  WD: 0.28 mm
  Correction: apochromat

• UPLSAPO
  100x_oil

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  Mag: 100.0x | NA: 1.4
  Immersion: oil
  WD: 0.13 mm
  Correction: apochromat

🎛️ Filters & Splitters

• Optical Filters

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  • GFP

  
  EX: - | EM: 525/25

  • CY3

  
  EX: - | EM: 615/10

  • CY5

  
  EX: - | EM: 685/35

🔍 Magnification Changers

No magnification changers recorded.

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QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data

• 20x Air (20x_air)

• 60x Water (60x_water)

• 100x Oil (100x_oil)