3i CSU-W1 Spinning Disk

📊 View QC & Maintenance History

Current Status: 🟢 Online

Operational

Instrument ID

scope-3i-csu-w1-spinning-disk

Manufacturer

3i / Zeiss

Model

Marianas CSU-W1 Spinning Disk Confocal

Stand

inverted

Location

BioCity, 5th floor, room 5149

Purchased

—

Funding

—

Modalities

confocal_spinning_disk FRAP photoactivation

Modules

FRAP piezo_z_stage hardware_autofocus incubation

Capabilities

• Notes: Confocal spinning-disk microscope (inverted) with 50 µm pinholes. Optimized for fast, gentle imaging of live samples and large fields of view. Imaging modes: Confocal (spinning disk), Live-cell time-lapse, FRAP, Photoactivation.

Software Environment

• 3i SlideBook

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  Component: acquisition
  Version: 6

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Hardware Configuration

💡 Light Sources

• 405 nm

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  Type: laser
  Wavelength: 405 nm
  Power: 100 mW

• 488 nm

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  Type: laser
  Wavelength: 488 nm
  Power: 150 mW

• 561 nm

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  Type: laser
  Wavelength: 561 nm
  Power: 100 mW

• 640 nm

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  Type: laser
  Wavelength: 640 nm
  Power: 100 mW

• 730 nm

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  Type: laser
  Wavelength: 730 nm
  Power: 40 mW

📷 Detectors

• Hamamatsu Orca Flash4.0

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  Type: sCMOS
  2048 x 2048 pixels, 6.5 x 6.5 µm pixel size

• Photometrics Evolve

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  Type: EMCCD
  10 MHz back-illuminated EMCCD, 512 x 512 pixels, 16 x 16 µm pixel size

🔬 Objectives

• Plan Apochromat 10x
  10x_air

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  Mag: 10.0x | NA: 0.45
  Immersion: air
  WD: 2.0 mm
  Correction: plan_apochromat

• Plan Apochromat 20x
  20x_air

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  Mag: 20.0x | NA: 0.8
  Immersion: air
  WD: 0.55 mm
  Correction: plan_apochromat

• LD C-Apochromat 40x
  40x_water

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  Mag: 40.0x | NA: 1.1
  Immersion: water
  WD: 0.62 mm
  Correction: apochromat
  Capabilities: cover glass 0.17

• LD Plan-Neofluar 63x
  63x_air

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  Mag: 63.0x | NA: 0.75
  Immersion: air
  WD: 1.7 mm
  Correction: fluorite
  Capabilities: cover glass 0.75

• LD C-Apochromat 63x
  63x_water

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  Mag: 63.0x | NA: 1.15
  Immersion: water
  WD: 0.60 mm
  Correction: apochromat
  Capabilities: cover glass 0.17

• Plan Apochromat 63x
  63x_oil

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  Mag: 63.0x | NA: 1.4
  Immersion: oil
  WD: 0.19 mm
  Correction: plan_apochromat

• Plan Apochromat 100x
  100x_oil

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  Mag: 100.0x | NA: 1.4
  Immersion: oil
  WD: 0.17 mm
  Correction: plan_apochromat

🎛️ Filters & Splitters

• Optical Filters

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  • Quad-band Dichroic

  
  EX: 440/40; 521/21; 607/34; 700/45 | EM: -

  • DAPI

  
  EX: - | EM: 445/45

  • GFP

  
  EX: - | EM: 525/50

  • Cy3/Alexa 568

  
  EX: - | EM: 617/73 or 600/37

  • Cy5/Alexa 647

  
  EX: - | EM: 692/40

  • Alexa 750

  
  EX: - | EM: 809/81

🔍 Magnification Changers

No magnification changers recorded.

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QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data

• 10x Air (10x_air)

• 20x Air (20x_air)

• 40x Water (40x_water)

• 63x Air (63x_air)

• 63x Water (63x_water)

• 63x Oil (63x_oil)

• 100x Oil (100x_oil)