3i CSU-W1 Spinning Disk Med C

📊 View QC & Maintenance History

Current Status: 🟢 Online

Operational

Instrument ID

scope-3i-csu-w1-spinning-disk-med-c

Manufacturer

3i / Zeiss

Model

Marianas CSU-W1 Spinning Disk Confocal

Stand

inverted

Location

Medisiina C, 2nd floor, room C242

Purchased

2019

Funding

—

Modalities

confocal_spinning_disk widefield_fluorescence transmitted_brightfield phase_contrast

Modules

incubation hardware_autofocus FRAP

Capabilities

• Notes: Confocal spinning-disk microscope based on a Zeiss Axio Observer 7. Includes MESA Illumination Enhancement. Location: Medisiina C, 2nd floor, room C242.

Software Environment

• 3i SlideBook

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  Component: acquisition
  Version: 6

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Hardware Configuration

💡 Light Sources

• LaserStack v4

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  Type: laser
  Wavelength: 405 nm
  Power: 100 mW
  Measured Fiber Output: 46 mW

• LaserStack v4

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  Type: laser
  Wavelength: 488 nm
  Power: 150 mW
  Measured Fiber Output: 102 mW

• LaserStack v4

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  Type: laser
  Wavelength: 561 nm
  Power: 150 mW
  Measured Fiber Output: 105.5 mW

• LaserStack v4

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  Type: laser
  Wavelength: 640 nm
  Power: 100 mW
  Measured Fiber Output: 50.4 mW

• pE-300

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  Type: led

  
  White Light Source with Liquid Light Guide

📷 Detectors

• Photometrics Prime BSI sCMOS

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  Type: sCMOS
  2048x2048 pixels, 6.5 µm pixel size, 95% QE

🔬 Objectives

• EC Plan-Neofluar 5x/0.16 NA Ph1
  5x_air

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  Mag: 5.0x | NA: 0.16
  Immersion: air
  WD: 18.5 mm
  Correction: plan_fluorite
  Capabilities: Ph1

• Plan-Apochromat 10x/0.45 NA Ph1
  10x_air

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  Mag: 10.0x | NA: 0.45
  Immersion: air
  WD: 2.0 mm
  Correction: plan_apochromat
  Capabilities: Ph1

• Plan-Apochromat 20x/0.8 NA
  20x_air

  ---

  Mag: 20.0x | NA: 0.8
  Immersion: air
  WD: 0.55 mm
  Correction: plan_apochromat

• LD Plan-Neofluar 20x/0.4 NA Corr
  20x_air_2

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  Mag: 20.0x | NA: 0.4
  Immersion: air
  WD: 7.9 mm
  Correction: plan_fluorite
  Capabilities: Correction collar for 0-1.5mm glass

• LD Plan-Neofluar 40x/0.6 NA Corr
  40x_air

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  Mag: 40.0x | NA: 0.6
  Immersion: air
  WD: 2.9 mm
  Correction: plan_fluorite
  Capabilities: Correction collar for 0-1.5mm glass

• C-Apochromat 40x/1.2 W Corr
  40x_water

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  Mag: 40.0x | NA: 1.2
  Immersion: water
  WD: 0.28 mm
  Correction: apochromat
  Capabilities: Correction ring for 0.17 mm cover glass

• Plan-Apochromat 63x/1.4 NA Oil
  63x_oil

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  Mag: 63.0x | NA: 1.4
  Immersion: oil
  WD: 0.19 mm
  Correction: plan_apochromat

• alpha Plan-Apochromat 100x/1.46 NA Oil DIC
  100x_oil

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  Mag: 100.0x | NA: 1.46
  Immersion: oil
  WD: 0.11 mm
  Correction: plan_apochromat
  Capabilities: DIC

🎛️ Filters & Splitters

• Optical Filters

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  • CSU-W1 Dichroic

  
  EX: 405/488/568/647 | EM: -

  • Quad-band Emitter

  
  EX: - | EM: 440/521/607/700

  • DAPI

  
  EX: - | EM: 445/45

  • GFP

  
  EX: - | EM: 525/30

  • Cy3/Alexa 568

  
  EX: - | EM: 617/73

  • Cy5/Alexa 647

  
  EX: - | EM: 692/40

  • DAPI Cube

  • FITC Cube

  • TRITC Cube

🔍 Magnification Changers

No magnification changers recorded.

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QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data

• 5x Air (5x_air)

• 10x Air (10x_air)

• 20x Air (20x_air)

• 20x Air (20x_air_2)

• 40x Air (40x_air)

• 40x Water (40x_water)

• 63x Oil (63x_oil)

• 100x Oil (100x_oil)