Lambert FLIM

📊 View QC & Maintenance History

Current Status: 🟢 Online

Operational

Instrument ID

scope-lambert-flim-frequency-domain

Manufacturer

Lambert Instruments

Model

LIFA (frequency domain FLIM)

Stand

inverted

Location

BioCity, 5th floor, room 5149

Purchased

—

Funding

—

Modalities

widefield_fluorescence FLIM

Modules

FLIM

Capabilities

• Notes: Fluorescence lifetime imaging microscopy (FLIM) measures differences in fluorescence decay lifetimes. The Lambert Instruments LIFA frequency-domain FLIM system is attached to an inverted Carl Zeiss AxioImager microscope body and supports fixed-sample imaging in widefield mode. It is suitable for FRET imaging, where donor lifetime decreases upon FRET; CFP-YFP and GFP-DsRed pairs have been used successfully. Lifetime imaging performance is <100 ps resolution over an approximately 0.1 ns to 1 ms range. The system is capable of high-content imaging. Recommended background reading: Bastiaens and Squire, "Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell" (Trends Cell Biol. 1999 Feb;9(2):48-52).

Software Environment

• Unknown

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  Component: acquisition
  

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Hardware Configuration

💡 Light Sources

• Multi-LED excitation

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  Type: led
  Wavelength: 406 nm

  
  Excitation line

• Multi-LED excitation

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  Type: led
  Wavelength: 469 nm

  
  Excitation line

• Multi-LED excitation

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  Type: led
  Wavelength: 533 nm

  
  Excitation line

📷 Detectors

No detectors recorded.

🔬 Objectives

No objectives recorded.

🎛️ Filters & Splitters

• Optical Components

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  No filters or splitters recorded.

🔍 Magnification Changers

No magnification changers recorded.

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QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

No objectives defined for this instrument.