Skip to content

Leica Thunder

📊 View QC & Maintenance History

Current Status: 🟢 Online

Operational

Leica Thunder
Instrument ID
scope-leica-thunder
Manufacturer
Leica Microsystems
Model
Leica THUNDER Imager 3D Live Cell
Stand
inverted
Location
—
Purchased
2023
Funding
—
Modalities
widefield_fluorescence transmitted_brightfield
Modules
hardware_autofocus

Capabilities

  • Notes: Widefield microscope with Leica Computational Clearing for real-time out-of-focus blur removal in 3D fluorescence imaging; high sensitivity supports low phototoxicity and photobleaching. LAS X raw data is saved separately; processing can be done during acquisition or by post-processing.

Software Environment

Hardware Configuration

💡 Light Sources

  • LED 8

    Type: led
    Wavelength: 395/25 nm
    Power: 200 mW
    390 in software

  • LED 8

    Type: led
    Wavelength: 438/29 nm
    Power: 200 mW
    440 in software

  • LED 8

    Type: led
    Wavelength: 475/28 nm
    Power: 200 mW

  • LED 8

    Type: led
    Wavelength: 511/16 nm
    Power: 200 mW
    510 in software

  • LED 8

    Type: led
    Wavelength: 555/28 nm
    Power: 200 mW

  • LED 8

    Type: led
    Wavelength: 575/25 nm
    Power: 200 mW

  • LED 8

    Type: led
    Wavelength: 635/22 nm
    Power: 200 mW

  • LED 8

    Type: led
    Wavelength: 730/40 nm
    Power: 200 mW
    747 in software

📷 Detectors

  • Leica K8

    Type: sCMOS
    monochrome; 18.8 mm sensor, 2048x2048 px, 6.5 um pixel, QE 95% peak, dark current 1.5 e-/pix/s, read noise (12-bit) 1.0 e- median / 1.1 e- RMS

  • Leica K3C

    Type: CMOS
    color CMOS; excellent color reproduction; 12-bit/channel RGB quantifiable images

🔬 Objectives

  • N PLAN 5x/0.12 PH 0
    5x_air

    Mag: 5.0x | NA: 0.12
    Immersion: air
    WD: 14.0 mm
    Correction: plan_achromat
    Capabilities: Phase Contrast (PH 0) DIC Prism B UV ≥365 nm
    FN 22

  • HC PL APO 10x/0.45
    10x_air

    Mag: 10.0x | NA: 0.45
    Immersion: air
    WD: 2.8 mm
    Correction: plan_apochromat
    Capabilities: DIC Prism A UV ≥340 nm
    FN 25

  • HC PL FLUOTAR L 20x/0.40 CORR
    20x_air

    Mag: 20.0x | NA: 0.4
    Immersion: air
    WD: 7.5 - 6.2 mm
    Correction: fluorite
    Capabilities: Correction Ring DIC Prism C UV ≥340 nm
    FN 25

  • HC PL APO 20x/0.70 IMM CORR
    20x_multi

    Mag: 20.0x | NA: 0.7
    Immersion: multi
    WD: 0.66 mm
    Correction: plan_apochromat
    Capabilities: Multi-immersion Correction Ring DIC Prism C UV ≥365 nm
    FN 25; W/O/G Immersion

  • HC PL APO 20x/0.80
    20x_air_2

    Mag: 20.0x | NA: 0.8
    Immersion: air
    WD: 0.4 mm
    Correction: plan_apochromat
    Capabilities: DIC Prism C UV ≥365 nm
    FN 25

  • HC PL APO 63x/1.40 OIL CS2
    63x_oil

    Mag: 63.0x | NA: 1.4
    Immersion: oil
    WD: 0.14 mm
    Correction: plan_apochromat
    Capabilities: DIC Prism E UV ≥340 nm
    FN 25

  • HC FLUOTAR L 16x/0.60 IMM CORR VISIR Ask staff
    16x_multi

    Mag: 16.0x | NA: 0.6
    Immersion: multi
    WD: 2.5 mm
    Correction: fluorite
    Capabilities: Multi-immersion (BABB/W/Glyc/Sil-Oil) IR-compatible to 1300 nm VISIR Correction Ring DIC Prism D
    FN 20

  • HC PL APO FLUOTAR 10x/0.30 PH 1 Ask staff
    10x_air_2

    Mag: 10.0x | NA: 0.3
    Immersion: air
    WD: 11.0 mm
    Correction: plan_apochromat
    Capabilities: Phase Contrast (PH 1) DIC Prism D UV ≥340 nm
    FN 25; from SP5?

  • HC PL APO 100x/1.40 OIL STED Ask staff
    100x_oil

    Mag: 100.0x | NA: 1.4
    Immersion: oil
    WD: 0.13 mm
    Correction: plan_apochromat
    Capabilities: STED DIC Prism D UV ≥340 nm
    FN 25; from SP5?

🎛️ Filters & Splitters

  • Optical Filters

    • CYR71010


    EX: 436/28; 506/21; 578/24; 730/40 | EM: 473/22; 539/24; 641/78; 810/80

    • DFT51010


    EX: 391/32; 479/33; 554/24; 638/31 | EM: 435/30; 519/25; 594/32; 695/58

    • CY3


    EX: 546/10 | EM: 572/23

    • A594


    EX: 592/21 | EM: 630/30

    • EMP_BF

    • EMP+Pol

    • Standalone Emission Filters


    EX: - | EM: 460/80; 535/70; 590/50; 642/80

🔍 Magnification Changers

No magnification changers recorded.

QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data

  • 5x Air (5x_air)

  • 10x Air (10x_air)

  • 20x Air (20x_air)

  • 20x Multi (20x_multi)

  • 20x Air (20x_air_2)

  • 63x Oil (63x_oil)

  • 16x Multi (16x_multi)

  • 10x Air (10x_air_2)

  • 100x Oil (100x_oil)