MSquared Aurora Airy Beam
Current Status: 🟢 Online
Operational
scope-msquared-aurora-airy-beamCapabilities
- Notes: Light-sheet fluorescence microscope using Airy-beam illumination for long-term volumetric live imaging with reduced photobleaching and striping artefacts. Approximate performance claims: up to 800 µm FOV (with 15.3x Special Optics), sub-1 µm axial resolution, and lower phototoxic burden due to single exposure per z-slice and self-healing/non-diffractive beam propagation.
Software Environment
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M Squared Cubes Acquisition
Component: acquisition
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M Squared Cubes Deconvolution
Component: deconvolution
Hardware Configuration
💡 Light Sources
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488 nm laser
Type: laser
Wavelength:488 nm
Power:100 mW -
561 nm laser
Type: laser
Wavelength:561 nm
Power:80 mW -
640 nm laser (LX)
Type: laser
Wavelength:640 nm
Power:75 mW
FIBER PIGTAIL:UFC: Galaxy
📷 Detectors
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Hamamatsu ORCA-Flash4.0 V3 (C11440-22CU)
Type: sCMOS
2048x2048. Supplied with water chiller for camera chip cooling.
🔬 Objectives
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54-10-12 Airy beam dipping objective
17x_multi
Mag:
17.0x| NA:0.4
Immersion: multi
WD: 12 mm
Capabilities:Cleared Tissue Imaging (CLARITY and TDE)
Excitation and detection objectives designed for dipping media with refractive index range 1.33-1.56 (NA 0.37-0.43), magnification range 15.3x-17.9x across RI. FOV 1.2 mm diameter.
🎛️ Filters & Splitters
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Optical Filters
- GFP filter
EX: - | EM: 520/40- GFP longpass
EX: - | EM: LP 500- RFP longpass
EX: - | EM: LP 570
🔍 Magnification Changers
No magnification changers recorded.
QC Metrics: Latest Measurements
🔦 Laser Performance
No laser performance metrics recorded recently.
🔬 Optical Resolution (PSF & Alignment)
No recent PSF or chromatic shift metrics available for any objectives.
Objectives without recent data
- 17x Multi (
17x_multi)