Skip to content

Nikon Ti2-E Crest V3

📊 View QC & Maintenance History

Current Status: 🟢 Online

Operational

Nikon Ti2-E Crest V3
Instrument ID
scope-nikon-crest-v3
Manufacturer
Nikon / CrestOptics
Model
Eclipse Ti2-E with X-Light V3 HTDS
Stand
inverted
Location
—
Purchased
—
Funding
—
Modalities
confocal_spinning_disk widefield_fluorescence transmitted_brightfield
Modules
hardware_autofocus incubation piezo_z_stage

Capabilities

  • Notes: High-end spinning disk system with Lumencor Celesta 7-channel laser, dual Photometrics Kinetix cameras, and Dark Enclosure WarmBox.

Software Environment

  • NIS-Elements AR

    Component: acquisition
    Version: Includes 6D, JOBS 2.0, 3D Measurement, and RT Acquisition

Hardware Configuration

💡 Light Sources

  • Celesta 7ch with Despeckler

    Type: laser
    Wavelength: 405 nm
    Power: ~800 mW
    Integral filter: 405/12

  • Celesta 7ch

    Type: laser
    Wavelength: 446 nm
    Power: ~800 mW
    Integral filter: 446/12

  • Celesta 7ch

    Type: laser
    Wavelength: 477 nm
    Power: ~800 mW
    Integral filter: 476/12

  • Celesta 7ch

    Type: laser
    Wavelength: 520 nm
    Power: ~800 mW
    Integral filter: 518/12

  • Celesta 7ch

    Type: laser
    Wavelength: 546 nm
    Power: ~800 mW
    Integral filter: 545/12

  • Celesta 7ch

    Type: laser
    Wavelength: 638 nm
    Power: ~800 mW
    Integral filter: 637/12

  • Celesta 7ch

    Type: laser
    Wavelength: 749 nm
    Power: ~800 mW
    Integral filter: 748/12

  • D-LEDI Fluorescence LED Illumination System

    Type: led
    Wavelength: broadband nm


    For widefield epifluorescence

  • T12-D-LHLED LED Lamp House

    Type: led
    Wavelength: white nm


    For DIA transmitted illumination

📷 Detectors

🔬 Objectives

  • CFI Plan Apochromat Lambda D 4X
    4x_air

    Mag: 4.0x | NA: 0.2
    Immersion: air
    WD: 20.0 mm
    Correction: plan_apochromat
    Capabilities: Lambda D

  • CFI Plan Apochromat Lambda D 20X
    20x_air

    Mag: 20.0x | NA: 0.8
    Immersion: air
    WD: 0.8 mm
    Correction: plan_apochromat
    Capabilities: Lambda D

  • CFI Plan Apochromat Lambda S 25XC Sil
    25x_silicone

    Mag: 25.0x | NA: 1.05
    Immersion: silicone
    WD: 0.55 mm
    Correction: plan_apochromat
    Capabilities: Lambda S

  • CFI Apochromat LWD Lambda S 40XC WI
    40x_water

    Mag: 40.0x | NA: 1.15
    Immersion: water
    WD: 0.59 - 0.61 mm
    Correction: apochromat
    Capabilities: Lambda S LWD

  • CFI Plan Apochromat Lambda D 60X Oil
    60x_oil

    Mag: 60.0x | NA: 1.42
    Immersion: oil
    WD: 0.15 mm
    Correction: plan_apochromat
    Capabilities: Lambda D

  • CFI Plan Apochromat Lambda S 60XC Sil
    60x_silicone

    Mag: 60.0x | NA: 1.3
    Immersion: silicone
    WD: 0.30 mm
    Correction: plan_apochromat
    Capabilities: Lambda S

  • CFI Plan Apochromat Lambda D 100x Oil
    100x_oil

    Mag: 100.0x | NA: 1.45
    Immersion: oil
    WD: 0.13 mm
    Correction: plan_apochromat
    Capabilities: Lambda D

🎛️ Filters & Splitters

  • Optical Filters

    • DAPI Ti2 32mm Cube SB LFOV

    • FITC Ti2 32mm Cube SB LFOV

    • mCherry Ti2 32mm Cube SB LFOV

    • Cy5 Ti2 32mm Cube SB LFOV

    • Celesta-DA/FI/TR/Cy5/Cy7-A (Full Multiband Penta)


    EX: FF01-391/477/549/639/741 | EM: -

    • Celesta CFP-YFP-A (CFP-YFP Multiband)


    EX: FF01-449/520 | EM: -

    • Neutral Density

    • Celesta-DA/FI/TR/Cy5/Cy7-A (Full Multiband Penta)

    • Celesta CFP-YFP-A (CFP-YFP Multiband)

    • Celesta-DA/FI/TR/Cy5/Cy7-A (Full Multiband Penta)


    EX: - | EM: FF01-411/511/593/684/817

    • Celesta CFP-YFP-A (CFP-YFP Multiband)


    EX: - | EM: FF01-484/561

    • Semrock FF02-438/24


    EX: - | EM: 438/24

    • Semrock FF02-485/20


    EX: - | EM: 485/20

    • Semrock FF01-511/20


    EX: - | EM: 511/20

    • Semrock FF01-560/25


    EX: - | EM: 560/25

    • Semrock FF01-595-31


    EX: - | EM: 595/31

    • Semrock FF02-685/40


    EX: - | EM: 685/40

  • Splitters / Dichroics

    • Nikon DIA
      Type: emission_splitter

    • MXR00546 Celesta DAPI/Cy3 - GFP/Cy5
      Type: emission_splitter

🔍 Magnification Changers

No magnification changers recorded.

QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

60x Oil (60x_oil)

  • PSF XY Max FWHM (60x Oil, 525nm)

    291.1
    psf.60x_oil.525.fwhm_xy_max_nm

  • PSF XY Min FWHM (60x Oil, 525nm)

    260.2
    psf.60x_oil.525.fwhm_xy_min_nm

  • PSF Z FWHM (60x Oil, 525nm)

    686.8
    psf.60x_oil.525.fwhm_z_nm

  • Asymmetry

    0.93
    psf.60x_oil.525.asymmetry

  • Z Nm

    118.7
    chromatic_shift.60x_oil.425_to_525.z_nm

60x Silicone (60x_silicone)

  • Fwhm Xy Max Nm

    288.3
    psf.60x_silicone.525.fwhm_xy_max_nm

  • Fwhm Xy Min Nm

    267.1
    psf.60x_silicone.525.fwhm_xy_min_nm

  • Fwhm Z Nm

    853.2
    psf.60x_silicone.525.fwhm_z_nm

  • Asymmetry

    0.93
    psf.60x_silicone.525.asymmetry

  • Z Nm

    127.5
    chromatic_shift.60x_silicone.425_to_525.z_nm

100x Oil (100x_oil)

  • Fwhm Xy Max Nm

    210.0
    psf.100x_oil.525.fwhm_xy_max_nm

  • Fwhm Xy Min Nm

    203.1
    psf.100x_oil.525.fwhm_xy_min_nm

  • Fwhm Z Nm

    585.5
    psf.100x_oil.525.fwhm_z_nm

  • Asymmetry

    0.97
    psf.100x_oil.525.asymmetry

  • Z Nm

    -25.4
    chromatic_shift.100x_oil.425_to_525.z_nm

Objectives without recent data

  • 4x Air (4x_air)

  • 20x Air (20x_air)

  • 25x Silicone (25x_silicone)

  • 40x Water (40x_water)