Nikon Eclipse Ti2-E

📊 View QC & Maintenance History

Current Status: 🟢 Online

Operational

Instrument ID

scope-nikon-eclipse-ti2-e

Manufacturer

Nikon

Model

Eclipse Ti2-E

Stand

inverted

Location

—

Purchased

2017

Funding

—

Modalities

widefield_fluorescence transmitted_brightfield DIC

Modules

incubation hardware_autofocus

Capabilities

Notes: Automated inverted microscope (high-content). Imaging modes: Widefield fluorescence (multichannel spectral imaging), Brightfield (and DIC), Time-lapse live-cell imaging (with autofocus), High-content screening (multi-well scanning).

Software Environment

Nikon NIS-Elements AR

Component: acquisition
Version: 5.11

Hardware Configuration

💡 Light Sources

Spectra X LED system

Type: led
Wavelength: 395/25; 440/20; 475/28; 550/15; 575/35; 640/30; 730/40 nm

550 nm module available by request

📷 Detectors

Hamamatsu Orca Flash4.0 V3

Type: sCMOS
sCMOS (2048x2044)
Nikon DS-Fi3

Type: sCMOS
Color CMOS (2880x2048)

🔬 Objectives

Plan UW 2x/0.06
2x_air

Mag: 2.0x | NA: 0.06
Immersion: air
WD: 7.5 mm
Correction: plan_achromat
Capabilities: BF (Recommended) FLUO (VIS) (Possible) Polarization (Possible)
Plan Fluor 4x/0.13
4x_air

Mag: 4.0x | NA: 0.13
Immersion: air
WD: 17.2 mm
Correction: plan_fluorite
Capabilities: BF (Recommended) FLUO (VIS) (Recommended) FLUO (UV) (Recommended) Polarization (Possible)
Plan Apo λD 4x/0.20
4x_air_2

Mag: 4.0x | NA: 0.2
Immersion: air
WD: 20.0 mm
Correction: plan_apochromat
Capabilities: Lambda D BF (Recommended) FLUO (VIS) (Recommended) FLUO (NIR) (Recommended)
Plan Fluor 10x/0.30 OFN 25 Ph1 DL
10x_air

Mag: 10.0x | NA: 0.3
Immersion: air
WD: 15.2 mm
Correction: plan_fluorite
Capabilities: Phase Contrast (PH1 DL) BF (Suitable) FLUO (VIS) (Suitable) FLUO (UV) (Suitable)
OFN 25
S Plan Fluor ELWD ADM 20x/0.45 DIC N1
20x_air

Mag: 20.0x | NA: 0.45
Immersion: air
WD: 8.2 - 6.9 mm
Correction: plan_fluorite
Capabilities: ELWD ADM DIC N1 (Suitable) BF (Recommended) FLUO (VIS) (Recommended) FLUO (UV) (Recommended) Polarization (Suitable)
Plan Apo λ 20x/0.75 OFN25 DIC N2
20x_air_2

Mag: 20.0x | NA: 0.75
Immersion: air
WD: 1.0 mm
Correction: plan_apochromat
Capabilities: Lambda DIC N2 (Suitable) BF (Recommended) FLUO (VIS) (Recommended) FLUO (UV) (Recommended) Polarization (Possible)
OFN 25
S Plan Fluor ELWD 40x/0.60 OFN22 Ph2
40x_air

Mag: 40.0x | NA: 0.6
Immersion: air
WD: 3.6 - 2.8 mm
Correction: plan_fluorite
Capabilities: ELWD Phase Contrast (PH2) BF (Suitable) FLUO (VIS) (Suitable) FLUO (UV) (Suitable)
OFN 22
Plan Apo λ 60x/1.40 Oil OFN25 DIC N2
60x_oil

Mag: 60.0x | NA: 1.4
Immersion: oil
WD: 0.13 mm
Correction: plan_apochromat
Capabilities: Lambda DIC N2 (Recommended) BF (Recommended) FLUO (VIS) (Recommended) FLUO (NIR) (Recommended) Polarization (Suitable)
OFN 25
Apo TIRF 100x/1.49 Oil
100x_oil

Mag: 100.0x | NA: 1.49
Immersion: oil
WD: 0.16 - 0.10 mm
Correction: apochromat
Capabilities: TIRF Phase Contrast (EXT PH4) DIC (Suitable) BF (Recommended) FLUO (VIS) (Recommended) FLUO (UV) (Possible) Polarization (Suitable)

🎛️ Filters & Splitters

Optical Filters
- Chroma 89403bs
- Chroma 84000v2
- Chroma 96377
EX: - | EM: 809/81
- DAPI Emission
EX: - | EM: 435/26
- CFP Emission
EX: - | EM: 475/20
- GFP Emission
EX: - | EM: 515/30
- YFP Emission
EX: - | EM: 540/21
- TRITC Emission
EX: - | EM: 595/40
- mCherry Emission
EX: - | EM: 632/60
- Cy5 Emission
EX: - | EM: 705/71
- Lamp Filter Cubes
- DIC Fixed Analyzer

🔍 Magnification Changers

No magnification changers recorded.

QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data

2x Air (2x_air)
4x Air (4x_air)
4x Air (4x_air_2)
10x Air (10x_air)
20x Air (20x_air)
20x Air (20x_air_2)
40x Air (40x_air)
60x Oil (60x_oil)
100x Oil (100x_oil)