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ONI Nanoimager

📊 View QC & Maintenance History

Current Status: ðŸŸĒ Online

Operational

ONI Nanoimager
Instrument ID
scope-oni-nanoimager
Manufacturer
ONI
Model
Nanoimager
Stand
other
Location
—
Purchased
2022
Funding
—
Modalities
TIRF SMLM SPT FRET
Modules
Temperature Control

Capabilities

  • Notes: Super-resolution microscope (compact). Imaging modes: Single-molecule localization microscopy (2D & 3D SMLM), Single-particle tracking (3D SPT), TIRF imaging, FRET imaging. Field of view: 50 Ξm x 80 Ξm per channel. Simultaneous 2-channel imaging (<10 nm mapping accuracy). Equipped with a closed-loop piezo stage (20/20/10 mm XYZ travel, 1 nm resolution) and continuous/one-shot autofocus.

Software Environment

  • NimOS microscope control software

    Component: acquisition

  • CODI analysis software

    Component: analysis

Hardware Configuration

ðŸ’Ą Light Sources

  • 405 nm violet

    Type: laser (diode)
    Wavelength: 405 nm
    Power: 70 mW
    Power measured at the objective.

  • 488 nm blue

    Type: laser (diode)
    Wavelength: 488 nm
    Power: 250 mW
    Power measured at the objective.

  • 561 nm green

    Type: laser (DPSS)
    Wavelength: 561 nm
    Power: 250 mW
    Power measured at the objective. DPSS technology inherently takes ~30 sec to stabilize.

  • 640 nm red

    Type: laser (diode)
    Wavelength: 640 nm
    Power: 220 mW
    Power measured at the objective.

📷 Detectors

  • Hamamatsu ORCA-Flash4.0 V3

    Type: sCMOS
    Digital sCMOS camera, 100 fps full frame. Typically operated with 2x2 binning.

🔎 Objectives

  • UPLXAPO100XO
    100x_oil

    Mag: 100.0x | NA: 1.45
    Immersion: oil
    WD: 0.13 mm
    Correction: apochromat

🎛ïļ Filters & Splitters

  • Optical Filters

    • Channel 0 Dual-band


    EX: - | EM: 498-551; 576-620

    • Channel 1


    EX: - | EM: 665-705

🔍 Magnification Changers

  • Custom tube lens

    Manufacturer: ONI
    Magnification: 1.1x
    Custom tube lens provides 110x total magnification with the 100x objective. Combined with 2x2 binning, this yields a pixel size of ~117 nm.

QC Metrics: Latest Measurements

ðŸ”Ķ Laser Performance

No laser performance metrics recorded recently.

🔎 Optical Resolution (PSF & Alignment)

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data
  • 100x Oil (100x_oil)