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Test Scope X1

📊 View QC & Maintenance History

Current Status: 🟢 Online

Operational

Test Scope X1
Instrument ID
scope-testx1
Manufacturer
Custom
Model
X1 Research Platform
Stand
inverted
Location
—
Purchased
2024
Funding
Internal development budget
Modalities
widefield_fluorescence confocal_point transmitted_brightfield
Modules
hardware_autofocus incubation optogenetics

Capabilities

  • Notes: Synthetic test instrument for dashboard visualization and integration testing.

Software Environment

Hardware Configuration

💡 Light Sources

  • Sapphire 488

    Type: laser
    Wavelength: 488 nm
    Power: 50 mW
    Pulsed mode active

  • pE-300

    Type: led
    Wavelength: multi-band nm
    Power: 25 mW
    Software alias: 'CoolLED_Main'

📷 Detectors

  • Hamamatsu ORCA-Flash4.0 V3

    Type: sCMOS
    Master detector

  • Custom Photonics PMT-R928

    Type: PMT
    Confocal channel A

🔬 Objectives

  • UPlanSApo 20x/0.75
    20x_air

    Mag: 20.0x | NA: 0.75
    Immersion: air
    WD: 0.60 mm
    Correction: plan_apochromat


    General purpose testing

  • UPlanSApo 60x/1.35 Ask staff
    60x_oil

    Mag: 60.0x | NA: 1.35
    Immersion: oil
    WD: 0.15 mm
    Correction: plan_apochromat
    Capabilities: High-NA test

🎛️ Filters & Splitters

  • Optical Filters


    EX: 405/20; 488/20; 561/20; 640/20 | EM: 450/50; 525/50; 600/50; 680/50

  • Splitters / Dichroics

    • Custom 50/50
      Type: emission_splitter

🔍 Magnification Changers

No magnification changers recorded.

QC Metrics: Latest Measurements

🔦 Laser Performance

  • Laser Linearity 488nm (R²)

    0.997
    laser.488.linearity_r2

🔬 Optical Resolution (PSF & Alignment)

60x Oil (60x_oil)

  • PSF XY Max FWHM (60x Oil, 525nm)

    240
    psf.60x_oil.525.fwhm_xy_max_nm

  • PSF XY Min FWHM (60x Oil, 525nm)

    240
    psf.60x_oil.525.fwhm_xy_min_nm

  • PSF Z FWHM (60x Oil, 525nm)

    600
    psf.60x_oil.525.fwhm_z_nm

  • Chromatic Shift 561→488 (60x Oil, nm)

    60
    chromatic_shift.60x_oil.561_to_488.dist_nm

Objectives without recent data
  • 20x Air (20x_air)

⚙️ Environment & Stage Performance

  • Stage Repeatability σX (nm)

    200
    stage.repeatability_sigma_x_nm

  • Stage Repeatability σY (nm)

    210
    stage.repeatability_sigma_y_nm