Zeiss TIRF

Current Status: 🟡 Warning

TIRF modality is no longer working due to a hardware fault.

Instrument ID

scope-zeiss-tirf

Manufacturer

Zeiss

Model

TIRF

Stand

inverted

Location

BioCity, 2nd floor, room 2209

Purchased

—

Funding

—

Modalities

widefield_fluorescence transmitted_brightfield TIRF

Modules

incubation hardware_autofocus motorized_stage

Capabilities

Notes: TIRF/widefield microscope (inverted). TIRF imaging enables approximately 70-150 nm axial (Z-axis) resolution near the basal cell membrane (vs. ~600 nm in common confocal microscopy). Designed for membrane studies such as endocytosis and integrin trafficking, and also suitable for sensitive widefield imaging.

Hamamatsu C9100-13

Type: EMCCD
EMCCD camera, 512x512 pixels
Hamamatsu ORCA-Flash4.0 CMOS

Type: sCMOS
sCMOS camera, 2048x2048 pixels

EC Epiplan-Neofluar
2.5x_air

Mag: 2.5x | NA: 0.06
Immersion: air
WD: 15.1 mm
EC Epiplan-Neofluar
10x_air

Mag: 10.0x | NA: 0.3
Immersion: air
WD: 5.2 mm
Plan Apochromat
20x_air

Mag: 20.0x | NA: 0.45
Immersion: air
WD: 8.2-6.9 mm
Correction: plan_apochromat
Alpha Plan Apochromat
63x_oil

Mag: 63.0x | NA: 1.46
Immersion: oil
WD: 0.55 mm
Correction: plan_apochromat
Capabilities: TIRF
Alpha Plan Apochromat
100x_oil

Mag: 100.0x | NA: 1.46
Immersion: oil
WD: 0.11 mm
Correction: plan_apochromat
Capabilities: TIRF

Optical Filters
- Filter set 38 HE (GFP)
EX: 470/40 | EM: 525/50
- Filter set 43 HE (DsRed)
EX: 550/25 | EM: 605/70
- Filter set 76 HE (CFP/GFP/DsRed)
EX: TBP 406+489+561 | EM: TBP 459+525+608
- Filter set 77 HE (GFP/mRFP/Alexa 633)
EX: TBP 483+564+642 | EM: TBP 526+601+688

No magnification changers recorded.

No laser performance metrics recorded recently.

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data