📝 Event: 2026-05-13_vendor_pm_qc

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📊 Report Overview

Event Metadata

Record Type
Qc Session

Reason / Suite
Preventive_maintenance

Operator
Zeiss field service engineer

Instrument
scope-zeiss-lsm-880-with-airyscan

QC Summary & Evaluation

Summary: Vendor preventive-maintenance QC recorded laser power, detector SNR, stage position error, optical alignment, focus, and application-level checks. The scan-field test failed while the remaining recorded QC checks were in order.

🔬 Tests Performed

Test Type	Details
Laser Power	Vendor single-point laser-power measurements at the fiber output and objective plane, including argon operating current and lifetime counters.
Stage	Manual XY-stage function and 3x3 TileScanRotation.lvb position-error check at a 512-pixel frame size.
Alignment	Detector, collimator, spectral, beam-profile, AOTF, resolution, scan-field, pinhole, MBS, PMT, and Airyscan alignment/calibration checks.
Psf	Visible-channel application checks included beads and a PSF Z scan using the Plan-Apo 63x oil objective; both were recorded in order.

🔦 Laser Measurement Context

Field	Value
Measurement position	at objective
Position details	Objective-plane values were measured at 100% output using ZEN Spot scan and a Plan-Neofluar 10x test objective. Fiber-output measurements are preserved in inputs_human because the schema permits one primary measurement position per QC session.

📏 QC Measurements

Measurement	Value	Source	Details
405 nm laser power at objective (100%) `laser.at_obj.405.100pct.power_mw`	3.83 mW	Laser input	Objective-plane measurement; checklist result in order.
458 nm laser power at objective (100%) `laser.at_obj.458.100pct.power_mw`	0.19 mW	Laser input	Objective-plane argon-line measurement; checklist result in order.
488 nm laser power at objective (100%) `laser.at_obj.488.100pct.power_mw`	1.33 mW	Laser input	Objective-plane argon-line measurement; checklist result in order.
543 nm laser power at objective (100%) `laser.at_obj.543.100pct.power_mw`	0.27 mW	Laser input	Objective-plane measurement; checklist result in order.
633 nm laser power at objective (100%) `laser.at_obj.633.100pct.power_mw`	1.09 mW	Laser input	Objective-plane measurement; checklist result in order.
Laser · Fiber · Argon · Optimal Power mW `laser.fiber.argon.optimal_power_mw`	11.42 mW	Human input	Argon power at the documented optimal setting; checklist result in order.
Laser · Fiber · Argon · Optimal Tube Current A `laser.fiber.argon.optimal_tube_current_a`	6 A	Human input	Argon tube current at the documented optimal setting.
Laser · Argon · Total Operating Hours `laser.argon.total_operating_hours`	17550 h	Human input	Argon total operating-hours counter recorded by the vendor.
Laser · Argon · Hours Since Exchange `laser.argon.hours_since_exchange`	1903 h	Human input	Argon usage recorded since the previous exchange.
Laser · Fiber · 405 · Power mW `laser.fiber.405.power_mw`	13.55 mW	Human input	405 nm power measured at the fiber output.
Laser · Fiber · 543 · Power mW `laser.fiber.543.power_mw`	0.95 mW	Human input	543 nm power measured at the fiber output.
Laser · Fiber · 633 · Power mW `laser.fiber.633.power_mw`	5.12 mW	Human input	633 nm power measured at the fiber output.
Signal To Noise · Channel 1 Test Status `signal_to_noise.channel_1_test_status`	pass	Human input	Vendor SNR checklist result recorded in order.
Signal To Noise · Channel 2 Test Status `signal_to_noise.channel_2_test_status`	pass	Human input	Vendor SNR checklist result recorded in order.
Signal To Noise · Channel 3 Test Status `signal_to_noise.channel_3_test_status`	pass	Human input	Vendor SNR checklist result recorded in order.
Signal To Noise · 32 Channel Array Test Status `signal_to_noise.32_channel_array_test_status`	pass	Human input	Vendor SNR checklist result recorded in order.
Signal To Noise · Airyscan Test Status `signal_to_noise.airyscan_test_status`	pass	Human input	Vendor SNR checklist result recorded in order; no BiG SNR result was entered.
Stage · Tile Scan Error X px `stage.tile_scan_error_x_px`	2 px	Human input	Error in X before stitching in the 3x3 tile-scan test.
Stage · Tile Scan Error Y px `stage.tile_scan_error_y_px`	0 px	Human input	Error in Y before stitching in the 3x3 tile-scan test.
Stage · Tile Scan Error Total px `stage.tile_scan_error_total_px`	4 px	Human input	Vendor-reported total error; acceptance criterion was less than 6 px.

Additional Notes

Source date only; started_utc is normalized to 00:00 UTC. Objective-plane laser power was measured at 100% output with a Plan-Neofluar 10x test objective. The checklist records the instrument as not fully operational because of the failed scan-field test and separately notes a VM800 startup communication error. The signed source report is retained outside the public repository because it contains personal data.

📄 Raw YAML Ledger

View 2026-05-13_vendor_pm_qc.yaml

schema_version: 1
record_type: qc_session
qc_id: qc_scope-zeiss-lsm-880-with-airyscan_20260513T0000Z_vendor_pm_v1
microscope: scope-zeiss-lsm-880-with-airyscan
started_utc: '2026-05-13T00:00:00Z'
performed_by: Zeiss field service engineer
reason: preventive_maintenance
summary: >-
  Vendor preventive-maintenance QC recorded laser power, detector SNR, stage position
  error, optical alignment, focus, and application-level checks. The scan-field test
  failed while the remaining recorded QC checks were in order.
notes: >-
  Source date only; started_utc is normalized to 00:00 UTC. Objective-plane laser power
  was measured at 100% output with a Plan-Neofluar 10x test objective. The checklist
  records the instrument as not fully operational because of the failed scan-field test
  and separately notes a VM800 startup communication error. The signed source report is
  retained outside the public repository because it contains personal data.

performed:
  - qc_type: laser_power
    details: >-
      Vendor single-point laser-power measurements at the fiber output and objective
      plane, including argon operating current and lifetime counters.
  - qc_type: stage
    details: >-
      Manual XY-stage function and 3x3 TileScanRotation.lvb position-error check at a
      512-pixel frame size.
  - qc_type: alignment
    details: >-
      Detector, collimator, spectral, beam-profile, AOTF, resolution, scan-field,
      pinhole, MBS, PMT, and Airyscan alignment/calibration checks.
  - qc_type: psf
    details: >-
      Visible-channel application checks included beads and a PSF Z scan using the
      Plan-Apo 63x oil objective; both were recorded in order.

inputs_human:
  - metric_id: laser.fiber.argon.optimal_power_mw
    metric_class: laser_power
    value: 11.42
    unit: mW
    details: Argon power at the documented optimal setting; checklist result in order.
  - metric_id: laser.fiber.argon.optimal_tube_current_a
    metric_class: laser_power
    value: 6.0
    unit: A
    details: Argon tube current at the documented optimal setting.
  - metric_id: laser.argon.total_operating_hours
    metric_class: laser_power
    value: 17550
    unit: h
    details: Argon total operating-hours counter recorded by the vendor.
  - metric_id: laser.argon.hours_since_exchange
    metric_class: laser_power
    value: 1903
    unit: h
    details: Argon usage recorded since the previous exchange.
  - metric_id: laser.fiber.405.power_mw
    metric_class: laser_power
    value: 13.55
    unit: mW
    details: 405 nm power measured at the fiber output.
  - metric_id: laser.fiber.543.power_mw
    metric_class: laser_power
    value: 0.95
    unit: mW
    details: 543 nm power measured at the fiber output.
  - metric_id: laser.fiber.633.power_mw
    metric_class: laser_power
    value: 5.12
    unit: mW
    details: 633 nm power measured at the fiber output.
  - metric_id: signal_to_noise.channel_1_test_status
    metric_class: signal_to_noise
    value: pass
    unit: ''
    details: Vendor SNR checklist result recorded in order.
  - metric_id: signal_to_noise.channel_2_test_status
    metric_class: signal_to_noise
    value: pass
    unit: ''
    details: Vendor SNR checklist result recorded in order.
  - metric_id: signal_to_noise.channel_3_test_status
    metric_class: signal_to_noise
    value: pass
    unit: ''
    details: Vendor SNR checklist result recorded in order.
  - metric_id: signal_to_noise.32_channel_array_test_status
    metric_class: signal_to_noise
    value: pass
    unit: ''
    details: Vendor SNR checklist result recorded in order.
  - metric_id: signal_to_noise.airyscan_test_status
    metric_class: signal_to_noise
    value: pass
    unit: ''
    details: Vendor SNR checklist result recorded in order; no BiG SNR result was entered.
  - metric_id: stage.tile_scan_error_x_px
    metric_class: stage_repeatability
    value: 2
    unit: px
    details: Error in X before stitching in the 3x3 tile-scan test.
  - metric_id: stage.tile_scan_error_y_px
    metric_class: stage_repeatability
    value: 0
    unit: px
    details: Error in Y before stitching in the 3x3 tile-scan test.
  - metric_id: stage.tile_scan_error_total_px
    metric_class: stage_repeatability
    value: 4
    unit: px
    details: Vendor-reported total error; acceptance criterion was less than 6 px.

laser_inputs_human:
  measurement_position: at_objective
  measurement_position_details: >-
    Objective-plane values were measured at 100% output using ZEN Spot scan and a
    Plan-Neofluar 10x test objective. Fiber-output measurements are preserved in
    inputs_human because the schema permits one primary measurement position per QC
    session.
  single_point_measurements:
    - laser: '405'
      setpoint: 100
      setpoint_units: percent
      power: 3.83
      power_units: mW
      details: Objective-plane measurement; checklist result in order.
    - laser: '458'
      setpoint: 100
      setpoint_units: percent
      power: 0.19
      power_units: mW
      details: Objective-plane argon-line measurement; checklist result in order.
    - laser: '488'
      setpoint: 100
      setpoint_units: percent
      power: 1.33
      power_units: mW
      details: Objective-plane argon-line measurement; checklist result in order.
    - laser: '543'
      setpoint: 100
      setpoint_units: percent
      power: 0.27
      power_units: mW
      details: Objective-plane measurement; checklist result in order.
    - laser: '633'
      setpoint: 100
      setpoint_units: percent
      power: 1.09
      power_units: mW
      details: Objective-plane measurement; checklist result in order.
  linearity_series:
    - laser: '405'
      setpoint_units: percent
      power_units: mW
      points:
        - setpoint: 100
          power: 3.83
      details: Single-point 100% objective-plane measurement from the vendor checklist.
    - laser: '458'
      setpoint_units: percent
      power_units: mW
      points:
        - setpoint: 100
          power: 0.19
      details: Single-point 100% objective-plane argon-line measurement.
    - laser: '488'
      setpoint_units: percent
      power_units: mW
      points:
        - setpoint: 100
          power: 1.33
      details: Single-point 100% objective-plane argon-line measurement.
    - laser: '543'
      setpoint_units: percent
      power_units: mW
      points:
        - setpoint: 100
          power: 0.27
      details: Single-point 100% objective-plane measurement from the vendor checklist.
    - laser: '633'
      setpoint_units: percent
      power_units: mW
      points:
        - setpoint: 100
          power: 1.09
      details: Single-point 100% objective-plane measurement from the vendor checklist.
  stability_series: []