Nikon Ti2-E Crest V3

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Current Status: 🟢 Online

Operational

📊 View QC & Maintenance History 🔬 Open Virtual Microscope

Manufacturer

Nikon / CrestOptics

Model

Eclipse Ti2-E with X-Light V3 HTDS

Stand

Inverted

Eyepieces

Trinocular

Location

BioCity, 5th floor, room 5149

Purchased

2025

Funding

—

Modalities

Spinning-Disk Confocal Widefield Fluorescence Transmitted Brightfield

Modules

Hardware Autofocus Incubation Piezo Z-Stage Hardware Triggering Motorized Stage

Software Environment

• NIS-Elements AR
  Acquisition
  Role: Acquisition

• NIS-Elements AR
  Analysis
  Role: Analysis
  Notes: Includes 6D, JOBS 2.0, 3D Measurement, and RT Acquisition

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Hardware Configuration

🌡️ Environmental Control

• Environmental Control
  controlled temperature
  Temperature control: Yes
  Notes: Dark Enclosure WarmBox set for Ti2 (TI2WB-SP400NX-G)

💡 Light Sources

• 406 nm Laser Lumencor Celesta 7ch with Despeckler
  Lumencor
  Type: Laser
  Wavelength: 406 nm
  Power: 288 mW
  Notes: Measured Disk OUT (Dec 2025). Integral filter: 405/12

• 444 nm Laser Lumencor Celesta 7ch
  Lumencor
  Type: Laser
  Wavelength: 444 nm
  Power: 344 mW
  Notes: Measured Disk OUT (Dec 2025). Integral filter: 446/12

• 476 nm Laser Lumencor Celesta 7ch
  Lumencor
  Type: Laser
  Wavelength: 476 nm
  Power: 405 mW
  Notes: Measured Disk OUT (Dec 2025). Integral filter: 476/12

• 520 nm Laser Lumencor Celesta 7ch
  Lumencor
  Type: Laser
  Wavelength: 520 nm
  Power: 410 mW
  Notes: Measured Disk OUT (Dec 2025). Integral filter: 518/12

• 545 nm Laser Lumencor Celesta 7ch
  Lumencor
  Type: Laser
  Wavelength: 545 nm
  Power: 433 mW
  Notes: Measured Disk OUT (Dec 2025). Integral filter: 545/12

• 636 nm Laser Lumencor Celesta 7ch
  Lumencor
  Type: Laser
  Wavelength: 636 nm
  Power: 539 mW
  Notes: Measured Disk OUT (Dec 2025). Integral filter: 637/12

• 746 nm Laser Lumencor Celesta 7ch
  Lumencor
  Type: Laser
  Wavelength: 746 nm
  Power: 522 mW
  Notes: Measured Disk OUT (Dec 2025). Integral filter: 748/12

• LED Nikon D-LEDI Fluorescence LED Illumination System
  Nikon
  Type: LED
  Notes: For widefield epifluorescence. Broadband illumination.

• LED Nikon T12-D-LHLED LED Lamp House
  Nikon
  Type: LED
  Role: Transmitted Illumination
  Notes: For DIA transmitted illumination. White light source.

🧭 Scanner

• Spinning Disk Scanner
  Type: Spinning Disk Scanner
  Pinhole: 50 µm
  Notes: CrestOptics X-Light V3 HTDS (Laser-SP disk-Throughput-V2&V3 50:250um)

📷 Detectors

• Photometrics Kinetix
  Scientific CMOS
  Type: Scientific CMOS
  Pixel pitch: 6.5 µm
  Sensor format: 3200 x 3200
  QE peak: 95%
  Notes: Master Camera (1st F-cam) - GFP/Cy5 in DualCam mode

• Photometrics Kinetix
  Scientific CMOS
  Type: Scientific CMOS
  Pixel pitch: 6.5 µm
  Sensor format: 3200 x 3200
  QE peak: 95%
  Notes: Slave Camera (2nd F-cam) - DAPI/Cy3 in DualCam mode

🔬 Objectives

• CFI Plan Apochromat Lambda D 4X 4x/0.2 AIR
  Nikon
  Model: CFI Plan Apochromat Lambda D 4X
  Magnification / NA: 4x/0.2
  Immersion: Air
  Correction: Plan Apochromat
  Working distance: 20.0 mm
  Product code: MRD70040
  AFC compatible: No
  Installed: Yes
  Notes: Optical family: Lambda D. FOV 25.

• CFI Plan Apochromat Lambda D 20X DIC N2 20x/0.8 AIR
  Nikon
  Model: CFI Plan Apochromat Lambda D 20X DIC N2
  Magnification / NA: 20x/0.8
  Immersion: Air
  Correction: Plan Apochromat
  Working distance: 0.8 mm
  Product code: MRD70270
  AFC compatible: Yes
  Installed: Yes
  Specialties: dic
  Notes: Optical family: Lambda D. FOV 25. Requires DIC-prism N2.

• CFI Plan Apochromat Lambda S 25XC Sil DIC N2 25x/1.05 SILICONE
  Nikon
  Model: CFI Plan Apochromat Lambda S 25XC Sil DIC N2
  Magnification / NA: 25x/1.05
  Immersion: Silicone
  Correction: Plan Apochromat
  Working distance: 0.55 mm
  Product code: MRD73250
  AFC compatible: Yes
  Installed: Yes
  Specialties: dic
  Notes: Optical family: Lambda S. Requires DIC-prism N2.

• CFI Apochromat LWD Lambda S 40XC WI DIC N2 40x/1.15 WATER
  Nikon
  Model: CFI Apochromat LWD Lambda S 40XC WI DIC N2
  Magnification / NA: 40x/1.15
  Immersion: Water
  Correction: Apochromat
  Working distance: 0.60 mm
  Product code: MRD77410
  AFC compatible: Yes
  Installed: Yes
  Specialties: long_working_distance, dic
  Notes: Optical family: Lambda S. Chromatic correction: 405-950nm. Requires DIC-prism N2.

• CFI Plan Apochromat Lambda S 60XC Sil DIC N2 60x/1.3 SILICONE
  Nikon
  Model: CFI Plan Apochromat Lambda S 60XC Sil DIC N2
  Magnification / NA: 60x/1.3
  Immersion: Silicone
  Correction: Plan Apochromat
  Working distance: 0.30 mm
  Product code: MRD73600
  AFC compatible: Yes
  Installed: Yes
  Specialties: dic
  Notes: Optical family: Lambda S. Requires DIC-prism N2.

• CFI Plan Apochromat Lambda D 100x Oil 100x/1.45 OIL
  Nikon
  Model: CFI Plan Apochromat Lambda D 100x Oil
  Magnification / NA: 100x/1.45
  Immersion: Oil
  Correction: Plan Apochromat
  Working distance: 0.13 mm
  Product code: MRD71970
  AFC compatible: Yes
  Installed: Yes
  Notes: Optical family: Lambda D. FOV 25.

• Apo TIRF 100x/1.49 Oil DIC N2 100x/1.49 OILAsk Staff to Install
  Nikon
  Model: Apo TIRF 100x/1.49 Oil DIC N2
  Magnification / NA: 100x/1.49
  Immersion: Oil
  Correction: Apochromat
  Working distance: 0.13 mm
  Product code: MRD01991
  AFC compatible: Yes
  Installed: No
  Specialties: tirf, dic
  Notes: Optional objective. Requires DIC-prism N2.

🧭 Stages & Focus Drives

• Z Piezo Stage — Prior QG/Prior SP600 piezo kit
  Prior
  Type: Z Piezo Stage

⚙️ Advanced Hardware

• Infrared Reflection Autofocus
  Installed: Yes
  Type: Infrared Reflection Autofocus

🎛️ Optical Path Elements

• Epi Turret
  Cube
  Element type: Turret
  Pos 1: DAPI Ti2 32mm Cube SB LFOV (MXR00714) — emission 447/60 nm — For widefield imaging with D-LEDI. Excitation: 377/50 nm. Dichroic Cutoff: 409 nm.
  Pos 2: FITC Ti2 32mm Cube SB LFOV (MXR00716) — emission 525/50 nm — Excitation: 470/40 nm. Dichroic Cutoff: 495 nm.
  Pos 3: mCherry Ti2 32mm Cube SB LFOV (MXR00719) — emission 630/75 nm — Excitation: 560/40 nm. Dichroic Cutoff: 585 nm.
  Pos 4: Cy5 Ti2 32mm Cube SB LFOV (MXR00720) — emission 700/75 nm — Excitation: 620/60 nm. Dichroic Cutoff: 660 nm.

• Crest Excitation Wheel
  Excitation
  Element type: Filter Wheel
  Pos 1: Celesta-DA/FI/TR/Cy5/Cy7-A (MXR00543) — emission 391/54.4 nm, 477/20.4 nm, 549/25.7 nm, 638.5/27.6 nm, 741/45.1 nm
  Pos 2: Celesta CFP-YFP-A (MXR00544) — emission 449/26.7 nm, 520/27.9 nm
  Pos 3: Neutral Density — ND 1.3-5%T-25mm. 5% transmission to be used with widefield.

• Crest Dichroic Wheel
  Dichroic
  Element type: Filter Wheel
  Pos 1: Celesta-DA/FI/TR/Cy5/Cy7-A (MXR00543) — emission 441/36.1 nm, 511/34.3 nm, 592.5/45.2 nm, 684/44.4 nm, 817/78.8 nm — Explicit transmission windows are mapped. Reflection cutoffs: 421, 491, 567, 659, 776 nm.
  Pos 2: Celesta CFP-YFP-A (MXR00544) — emission 484/25.9 nm, 561/38.9 nm — Explicit transmission windows are mapped. Reflection cutoffs: 471, 539 nm.

• Crest Emission Wheel
  Emission
  Element type: Filter Wheel
  Pos 1: Celesta-DA/FI/TR/Cy5/Cy7-A (MXR00541 / MXR00543) — emission 441/36.1 nm, 511/34.3 nm, 592.5/45.2 nm, 684/44.4 nm, 817/78.8 nm
  Pos 2: Celesta CFP-YFP-A (MXR00542 / MXR00544) — emission 484/25.9 nm, 561/38.9 nm
  Pos 3: Bandpass — emission 438/24 nm
  Pos 4: Bandpass — emission 485/20 nm
  Pos 5: Bandpass — emission 511/20 nm
  Pos 6: Bandpass — emission 560/25 nm
  Pos 7: Bandpass — emission 595/31 nm
  Pos 8: Bandpass — emission 685/40 nm

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QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

CFI Plan Apochromat Lambda S 60XC Sil DIC N2 60x/1.3 SILICONE (60x_silicone)

• Fwhm Xy Max Nm

  ---

  288.3
  psf.60x_silicone.525.fwhm_xy_max_nm

• Fwhm Xy Min Nm

  ---

  267.1
  psf.60x_silicone.525.fwhm_xy_min_nm

• Fwhm Z Nm

  ---

  853.2
  psf.60x_silicone.525.fwhm_z_nm

• Asymmetry

  ---

  0.93
  psf.60x_silicone.525.asymmetry

• Z Nm

  ---

  127.5
  chromatic_shift.60x_silicone.425_to_525.z_nm

CFI Plan Apochromat Lambda D 100x Oil 100x/1.45 OIL (100x_oil)

• Fwhm Xy Max Nm

  ---

  210.0
  psf.100x_oil.525.fwhm_xy_max_nm

• Fwhm Xy Min Nm

  ---

  203.1
  psf.100x_oil.525.fwhm_xy_min_nm

• Fwhm Z Nm

  ---

  585.5
  psf.100x_oil.525.fwhm_z_nm

• Asymmetry

  ---

  0.97
  psf.100x_oil.525.asymmetry

• Z Nm

  ---

  -25.4
  chromatic_shift.100x_oil.425_to_525.z_nm

Objectives without recent data

• CFI Plan Apochromat Lambda D 4X 4x/0.2 AIR (4x_air)

• CFI Plan Apochromat Lambda D 20X DIC N2 20x/0.8 AIR (20x_air)

• CFI Plan Apochromat Lambda S 25XC Sil DIC N2 25x/1.05 SILICONE (25x_silicone)

• CFI Apochromat LWD Lambda S 40XC WI DIC N2 40x/1.15 WATER (40x_water)

• Apo TIRF 100x/1.49 Oil DIC N2 100x/1.49 OIL (100x_oil_tirf)

Metadata Completeness

• Missing conditional fields (currently applicable):

• software[].version — Software version

• Legacy aliases in use (fallback accepted):

• modules[].name → modules[].type

• hardware.detectors[].pixel_size_um → hardware.detectors[].pixel_pitch_um

• hardware.detectors[].min_nm → hardware.detectors[].collection_min_nm

• hardware.detectors[].max_nm → hardware.detectors[].collection_max_nm

• hardware.detectors[].channel_center_nm → hardware.detectors[].collection_center_nm

• hardware.detectors[].bandwidth_nm → hardware.detectors[].collection_width_nm