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Nikon Ti2-E Crest V3

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Current Status: 🟢 Online

Operational

📊 View QC & Maintenance History 🔬 Open Virtual Microscope

Nikon Ti2-E Crest V3
Manufacturer
Nikon / CrestOptics
Model
Eclipse Ti2-E with X-Light V3 HTDS
Stand
Inverted
Eyepieces
Trinocular
Location
BioCity, 5th floor, room 5149
Purchased
2025
Funding
—
Capabilities
Use these chips to quickly decide if this microscope supports your planned imaging mode, contrast method, or readout.
Confocal spinning diskWidefield fluorescenceTransmitted brightfieldDICLive cell imaging
Modules
Hardware Autofocus Incubation Piezo Z-Stage Hardware Triggering Motorized Stage


Software Environment

  • NIS-Elements AR
    Acquisition
    Role: Acquisition
    Version: Not documented

  • NIS-Elements AR
    Analysis
    Role: Analysis
    Version: Not documented
    Notes: Includes 6D, JOBS 2.0, 3D Measurement, and RT Acquisition

Hardware Configuration

🌡️ Environmental Control

  • Environmental Control
    controlled temperature
    Temperature control: Yes
    Notes: Dark Enclosure WarmBox set for Ti2 (TI2WB-SP400NX-G)

💡 Light Sources

  • 406 nm Laser Lumencor Celesta 7ch with Despeckler
    Lumencor
    Type: Laser
    Wavelength: 406 nm
    Power: 288 mW
    Notes: Measured Disk OUT (Dec 2025). Integral filter: 405/12

  • 444 nm Laser Lumencor Celesta 7ch
    Lumencor
    Type: Laser
    Wavelength: 444 nm
    Power: 344 mW
    Notes: Measured Disk OUT (Dec 2025). Integral filter: 446/12

  • 476 nm Laser Lumencor Celesta 7ch
    Lumencor
    Type: Laser
    Wavelength: 476 nm
    Power: 405 mW
    Notes: Measured Disk OUT (Dec 2025). Integral filter: 476/12

  • 520 nm Laser Lumencor Celesta 7ch
    Lumencor
    Type: Laser
    Wavelength: 520 nm
    Power: 410 mW
    Notes: Measured Disk OUT (Dec 2025). Integral filter: 518/12

  • 545 nm Laser Lumencor Celesta 7ch
    Lumencor
    Type: Laser
    Wavelength: 545 nm
    Power: 433 mW
    Notes: Measured Disk OUT (Dec 2025). Integral filter: 545/12

  • 636 nm Laser Lumencor Celesta 7ch
    Lumencor
    Type: Laser
    Wavelength: 636 nm
    Power: 539 mW
    Notes: Measured Disk OUT (Dec 2025). Integral filter: 637/12

  • 746 nm Laser Lumencor Celesta 7ch
    Lumencor
    Type: Laser
    Wavelength: 746 nm
    Power: 522 mW
    Notes: Measured Disk OUT (Dec 2025). Integral filter: 748/12

  • LED Nikon D-LEDI Fluorescence LED Illumination System
    Nikon
    Type: LED
    Notes: For widefield epifluorescence. Broadband illumination.

  • LED Nikon T12-D-LHLED LED Lamp House
    Nikon
    Type: LED
    Role: Transmitted Illumination
    Notes: For DIA transmitted illumination. White light source.

🧭 Scanner

  • Spinning Disk Scanner
    Type: Spinning Disk Scanner
    Pinhole: 50 µm
    Notes: CrestOptics X-Light V3 HTDS (Laser-SP disk-Throughput-V2&V3 50:250um)

📷 Detectors

  • Photometrics Kinetix
    Scientific CMOS
    Type: Scientific CMOS
    Pixel pitch: 6.5 µm
    Sensor format: 3200 x 3200
    QE peak: 95%
    Notes: Master Camera (1st F-cam) - GFP/Cy5 in DualCam mode

  • Photometrics Kinetix
    Scientific CMOS
    Type: Scientific CMOS
    Pixel pitch: 6.5 µm
    Sensor format: 3200 x 3200
    QE peak: 95%
    Notes: Slave Camera (2nd F-cam) - DAPI/Cy3 in DualCam mode

🔬 Objectives

  • CFI Plan Apochromat Lambda D 4X 4x/0.2 AIR
    Nikon
    Model: CFI Plan Apochromat Lambda D 4X
    Magnification / NA: 4x/0.2
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 20.0 mm
    Product code: MRD70040
    AFC compatible: No
    Installed: Yes
    Notes: Optical family: Lambda D. FOV 25.

  • CFI Plan Apochromat Lambda D 20X DIC N2 20x/0.8 AIR
    Nikon
    Model: CFI Plan Apochromat Lambda D 20X DIC N2
    Magnification / NA: 20x/0.8
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 0.8 mm
    Product code: MRD70270
    AFC compatible: Yes
    Installed: Yes
    Specialties: dic
    Notes: Optical family: Lambda D. FOV 25. Requires DIC-prism N2.

  • CFI Plan Apochromat Lambda S 25XC Sil DIC N2 25x/1.05 SILICONE
    Nikon
    Model: CFI Plan Apochromat Lambda S 25XC Sil DIC N2
    Magnification / NA: 25x/1.05
    Immersion: Silicone
    Correction: Plan Apochromat
    Working distance: 0.55 mm
    Product code: MRD73250
    AFC compatible: Yes
    Installed: Yes
    Specialties: dic
    Notes: Optical family: Lambda S. Requires DIC-prism N2.

  • CFI Apochromat LWD Lambda S 40XC WI DIC N2 40x/1.15 WATER
    Nikon
    Model: CFI Apochromat LWD Lambda S 40XC WI DIC N2
    Magnification / NA: 40x/1.15
    Immersion: Water
    Correction: Apochromat
    Working distance: 0.60 mm
    Product code: MRD77410
    AFC compatible: Yes
    Installed: Yes
    Specialties: long_working_distance, dic
    Notes: Optical family: Lambda S. Chromatic correction: 405-950nm. Requires DIC-prism N2.

  • CFI Plan Apochromat Lambda S 60XC Sil DIC N2 60x/1.3 SILICONE
    Nikon
    Model: CFI Plan Apochromat Lambda S 60XC Sil DIC N2
    Magnification / NA: 60x/1.3
    Immersion: Silicone
    Correction: Plan Apochromat
    Working distance: 0.30 mm
    Product code: MRD73600
    AFC compatible: Yes
    Installed: Yes
    Specialties: dic
    Notes: Optical family: Lambda S. Requires DIC-prism N2.

  • CFI Plan Apochromat Lambda D 100x Oil 100x/1.45 OIL
    Nikon
    Model: CFI Plan Apochromat Lambda D 100x Oil
    Magnification / NA: 100x/1.45
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.13 mm
    Product code: MRD71970
    AFC compatible: Yes
    Installed: Yes
    Notes: Optical family: Lambda D. FOV 25.

  • Apo TIRF 100x/1.49 Oil DIC N2 100x/1.49 OILAsk Staff to Install
    Nikon
    Model: Apo TIRF 100x/1.49 Oil DIC N2
    Magnification / NA: 100x/1.49
    Immersion: Oil
    Correction: Apochromat
    Working distance: 0.13 mm
    Product code: MRD01991
    AFC compatible: Yes
    Installed: No
    Specialties: tirf, dic
    Notes: Optional objective. Requires DIC-prism N2.

🧭 Stages & Focus Drives

  • Z Piezo Stage — Prior QG/Prior SP600 piezo kit
    Prior
    Type: Z Piezo Stage

⚙️ Advanced Hardware

  • Infrared Reflection Autofocus
    Installed: Yes
    Type: Infrared Reflection Autofocus

🎛️ Optical Path Elements

  • Epi Turret
    Cube
    Element type: Turret
    Pos 1: DAPI Ti2 32mm Cube SB LFOV (MXR00714) — emission 447/60 nm — For widefield imaging with D-LEDI. Excitation: 377/50 nm. Dichroic Cutoff: 409 nm.
    Pos 2: FITC Ti2 32mm Cube SB LFOV (MXR00716) — emission 525/50 nm — Excitation: 470/40 nm. Dichroic Cutoff: 495 nm.
    Pos 3: mCherry Ti2 32mm Cube SB LFOV (MXR00719) — emission 630/75 nm — Excitation: 560/40 nm. Dichroic Cutoff: 585 nm.
    Pos 4: Cy5 Ti2 32mm Cube SB LFOV (MXR00720) — emission 700/75 nm — Excitation: 620/60 nm. Dichroic Cutoff: 660 nm.

  • Crest Excitation Wheel
    Excitation
    Element type: Filter Wheel
    Pos 1: Celesta-DA/FI/TR/Cy5/Cy7-A (MXR00543) — emission 391/54.4 nm, 477/20.4 nm, 549/25.7 nm, 638.5/27.6 nm, 741/45.1 nm
    Pos 2: Celesta CFP-YFP-A (MXR00544) — emission 449/26.7 nm, 520/27.9 nm
    Pos 3: Neutral Density — ND 1.3-5%T-25mm. 5% transmission to be used with widefield.

  • Crest Dichroic Wheel
    Dichroic
    Element type: Filter Wheel
    Pos 1: Celesta-DA/FI/TR/Cy5/Cy7-A (MXR00543) — emission 441/36.1 nm, 511/34.3 nm, 592.5/45.2 nm, 684/44.4 nm, 817/78.8 nm — Explicit transmission windows are mapped. Reflection cutoffs: 421, 491, 567, 659, 776 nm.
    Pos 2: Celesta CFP-YFP-A (MXR00544) — emission 484/25.9 nm, 561/38.9 nm — Explicit transmission windows are mapped. Reflection cutoffs: 471, 539 nm.

  • Crest Emission Wheel
    Emission
    Element type: Filter Wheel
    Pos 1: Celesta-DA/FI/TR/Cy5/Cy7-A (MXR00541 / MXR00543) — emission 441/36.1 nm, 511/34.3 nm, 592.5/45.2 nm, 684/44.4 nm, 817/78.8 nm
    Pos 2: Celesta CFP-YFP-A (MXR00542 / MXR00544) — emission 484/25.9 nm, 561/38.9 nm
    Pos 3: Bandpass — emission 438/24 nm
    Pos 4: Bandpass — emission 485/20 nm
    Pos 5: Bandpass — emission 511/20 nm
    Pos 6: Bandpass — emission 560/25 nm
    Pos 7: Bandpass — emission 595/31 nm
    Pos 8: Bandpass — emission 685/40 nm

QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

CFI Plan Apochromat Lambda S 60XC Sil DIC N2 60x/1.3 SILICONE (60x_silicone)

  • Fwhm Xy Max Nm

    288.3
    psf.60x_silicone.525.fwhm_xy_max_nm

  • Fwhm Xy Min Nm

    267.1
    psf.60x_silicone.525.fwhm_xy_min_nm

  • Fwhm Z Nm

    853.2
    psf.60x_silicone.525.fwhm_z_nm

  • Asymmetry

    0.93
    psf.60x_silicone.525.asymmetry

  • Z Nm

    127.5
    chromatic_shift.60x_silicone.425_to_525.z_nm

CFI Plan Apochromat Lambda D 100x Oil 100x/1.45 OIL (100x_oil)

  • Fwhm Xy Max Nm

    210.0
    psf.100x_oil.525.fwhm_xy_max_nm

  • Fwhm Xy Min Nm

    203.1
    psf.100x_oil.525.fwhm_xy_min_nm

  • Fwhm Z Nm

    585.5
    psf.100x_oil.525.fwhm_z_nm

  • Asymmetry

    0.97
    psf.100x_oil.525.asymmetry

  • Z Nm

    -25.4
    chromatic_shift.100x_oil.425_to_525.z_nm

Objectives without recent data

  • CFI Plan Apochromat Lambda D 4X 4x/0.2 AIR (4x_air)

  • CFI Plan Apochromat Lambda D 20X DIC N2 20x/0.8 AIR (20x_air)

  • CFI Plan Apochromat Lambda S 25XC Sil DIC N2 25x/1.05 SILICONE (25x_silicone)

  • CFI Apochromat LWD Lambda S 40XC WI DIC N2 40x/1.15 WATER (40x_water)

  • Apo TIRF 100x/1.49 Oil DIC N2 100x/1.49 OIL (100x_oil_tirf)

Metadata Completeness

  • Missing conditional fields (currently applicable):

  • software[].version — Software version