Nikon Ti2-E Crest V3

About this microscope

Here you’ll find the hardware and capabilities currently recorded for this microscope. If you are planning an experiment, AIC Turku staff can help with access, training, and configuration choices.

Current status: 🟢 Online

Operational

QC and maintenance history Open Virtual Microscope

Manufacturer

Nikon / CrestOptics

Model

Eclipse Ti2-E with X-Light V3 HTDS

Stand

Inverted

Eyepieces

Trinocular

Location

BioCity, 5th floor, room 5149

Purchased

2025

Funding

—

Capabilities

These are the capabilities recorded for this microscope. Open the optical-route details below to see how the hardware is connected.

Confocal spinning diskWidefield fluorescenceTransmitted brightfieldDICLive cell imaging

Methods and the light path that records them

Each method below is explicitly associated in the instrument record with the physical light path that implements it. An imaging mode or contrast method that appears above but not here has no recorded method-to-path mapping yet.

• Spinning-disk confocal: Confocal spinning disk
• Widefield fluorescence: Widefield fluorescence
• Transmitted light: Transmitted brightfield, DIC

Modules

Hardware Autofocus Incubation Piezo Z-Stage Hardware Triggering Motorized Stage

Software

• NIS-Elements AR
  Acquisition
  Role: Acquisition
  Version: Not documented

• NIS-Elements AR
  Analysis
  Role: Analysis
  Version: Not documented
  Notes: Includes 6D, JOBS 2.0, 3D Measurement, and RT Acquisition

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Hardware

Environmental control

• Environmental Control
  controlled temperature
  Temperature control: Yes
  Notes: Dark Enclosure WarmBox set for Ti2 (TI2WB-SP400NX-G)

Light sources

• 406 nm Laser Lumencor Celesta 7ch with Despeckler
  Lumencor
  Type: Laser
  Wavelength: 406 nm
  Power: 288 mW
  Notes: Measured Disk OUT (Dec 2025). Integral filter: 405/12

• 444 nm Laser Lumencor Celesta 7ch
  Lumencor
  Type: Laser
  Wavelength: 444 nm
  Power: 344 mW
  Notes: Measured Disk OUT (Dec 2025). Integral filter: 446/12

• 476 nm Laser Lumencor Celesta 7ch
  Lumencor
  Type: Laser
  Wavelength: 476 nm
  Power: 405 mW
  Notes: Measured Disk OUT (Dec 2025). Integral filter: 476/12

• 520 nm Laser Lumencor Celesta 7ch
  Lumencor
  Type: Laser
  Wavelength: 520 nm
  Power: 410 mW
  Notes: Measured Disk OUT (Dec 2025). Integral filter: 518/12

• 545 nm Laser Lumencor Celesta 7ch
  Lumencor
  Type: Laser
  Wavelength: 545 nm
  Power: 433 mW
  Notes: Measured Disk OUT (Dec 2025). Integral filter: 545/12

• 636 nm Laser Lumencor Celesta 7ch
  Lumencor
  Type: Laser
  Wavelength: 636 nm
  Power: 539 mW
  Notes: Measured Disk OUT (Dec 2025). Integral filter: 637/12

• 746 nm Laser Lumencor Celesta 7ch
  Lumencor
  Type: Laser
  Wavelength: 746 nm
  Power: 522 mW
  Notes: Measured Disk OUT (Dec 2025). Integral filter: 748/12

• LED Nikon D-LEDI Fluorescence LED Illumination System
  Nikon
  Type: LED
  Notes: For widefield epifluorescence. Broadband illumination.

• LED Nikon T12-D-LHLED LED Lamp House
  Nikon
  Type: LED
  Role: Transmitted Illumination
  Notes: For DIA transmitted illumination. White light source.

Scanner

• Spinning Disk Scanner
  Type: Spinning Disk Scanner
  Pinhole: 50 µm
  Notes: CrestOptics X-Light V3 HTDS (Laser-SP disk-Throughput-V2&V3 50:250um)

Detectors

• Photometrics Kinetix
  Scientific CMOS
  Type: Scientific CMOS
  Pixel pitch: 6.5 µm
  Sensor format: 3200 x 3200
  QE peak: 95%
  Notes: Master Camera (1st F-cam) - GFP/Cy5 in DualCam mode

• Photometrics Kinetix
  Scientific CMOS
  Type: Scientific CMOS
  Pixel pitch: 6.5 µm
  Sensor format: 3200 x 3200
  QE peak: 95%
  Notes: Slave Camera (2nd F-cam) - DAPI/Cy3 in DualCam mode

Objectives

View this microscope's objectives in the catalogue

• CFI Plan Apochromat Lambda D 4X 4x/0.2 AIR
  Nikon
  Model: CFI Plan Apochromat Lambda D 4X
  Magnification / NA: 4x/0.2
  Immersion: Air
  Correction: Plan Apochromat
  Working distance: 20.0 mm
  Product code: MRD70040
  AFC compatible: No
  Installed: Yes
  Notes: Optical family: Lambda D. FOV 25.

• CFI Plan Apochromat Lambda D 20X DIC N2 20x/0.8 AIR
  Nikon
  Model: CFI Plan Apochromat Lambda D 20X DIC N2
  Magnification / NA: 20x/0.8
  Immersion: Air
  Correction: Plan Apochromat
  Working distance: 0.8 mm
  Product code: MRD70270
  AFC compatible: Yes
  Installed: Yes
  Specialties: dic
  Notes: Optical family: Lambda D. FOV 25. Requires DIC-prism N2.

• CFI Plan Apochromat Lambda S 25XC Sil DIC N2 25x/1.05 SILICONE
  Nikon
  Model: CFI Plan Apochromat Lambda S 25XC Sil DIC N2
  Magnification / NA: 25x/1.05
  Immersion: Silicone
  Correction: Plan Apochromat
  Working distance: 0.55 mm
  Product code: MRD73250
  AFC compatible: Yes
  Installed: Yes
  Specialties: dic
  Notes: Optical family: Lambda S. Requires DIC-prism N2.

• CFI Apochromat LWD Lambda S 40XC WI DIC N2 40x/1.15 WATER
  Nikon
  Model: CFI Apochromat LWD Lambda S 40XC WI DIC N2
  Magnification / NA: 40x/1.15
  Immersion: Water
  Correction: Apochromat
  Working distance: 0.60 mm
  Product code: MRD77410
  AFC compatible: Yes
  Installed: Yes
  Specialties: long_working_distance, dic
  Notes: Optical family: Lambda S. Chromatic correction: 405-950nm. Requires DIC-prism N2.

• CFI Plan Apochromat Lambda S 60XC Sil DIC N2 60x/1.3 SILICONE
  Nikon
  Model: CFI Plan Apochromat Lambda S 60XC Sil DIC N2
  Magnification / NA: 60x/1.3
  Immersion: Silicone
  Correction: Plan Apochromat
  Working distance: 0.30 mm
  Product code: MRD73600
  AFC compatible: Yes
  Installed: Yes
  Specialties: dic
  Notes: Optical family: Lambda S. Requires DIC-prism N2.

• CFI Plan Apochromat Lambda D 100x Oil 100x/1.45 OIL
  Nikon
  Model: CFI Plan Apochromat Lambda D 100x Oil
  Magnification / NA: 100x/1.45
  Immersion: Oil
  Correction: Plan Apochromat
  Working distance: 0.13 mm
  Product code: MRD71970
  AFC compatible: Yes
  Installed: Yes
  Notes: Optical family: Lambda D. FOV 25.

• Apo TIRF 100x/1.49 Oil DIC N2 OILNot installed
  Nikon
  Model: Apo TIRF 100x/1.49 Oil DIC N2
  Magnification / NA: 100x/1.49
  Immersion: Oil
  Correction: Apochromat
  Working distance: 0.13 mm
  Product code: MRD01991
  AFC compatible: Yes
  Installed: No
  Specialties: tirf, dic
  Notes: Optional objective. Requires DIC-prism N2.

Stages and focus drives

• Z Piezo Stage — Prior QG/Prior SP600 piezo kit
  Prior
  Type: Z Piezo Stage

Autofocus and triggering

• Infrared Reflection Autofocus
  Installed: Yes
  Type: Infrared Reflection Autofocus

Optical-path elements

• Epi Turret
  Cube
  Element type: Turret
  Pos 1: DAPI Ti2 32mm Cube SB LFOV (MXR00714) — emission 447/60 nm — For widefield imaging with D-LEDI. Excitation: 377/50 nm. Dichroic Cutoff: 409 nm.
  Pos 2: FITC Ti2 32mm Cube SB LFOV (MXR00716) — emission 525/50 nm — Excitation: 470/40 nm. Dichroic Cutoff: 495 nm.
  Pos 3: mCherry Ti2 32mm Cube SB LFOV (MXR00719) — emission 630/75 nm — Excitation: 560/40 nm. Dichroic Cutoff: 585 nm.
  Pos 4: Cy5 Ti2 32mm Cube SB LFOV (MXR00720) — emission 700/75 nm — Excitation: 620/60 nm. Dichroic Cutoff: 660 nm.

• Crest Excitation Wheel
  Excitation
  Element type: Filter Wheel
  Pos 1: Celesta-DA/FI/TR/Cy5/Cy7-A (MXR00543) — emission 391/54.4 nm, 477/20.4 nm, 549/25.7 nm, 638.5/27.6 nm, 741/45.1 nm
  Pos 2: Celesta CFP-YFP-A (MXR00544) — emission 449/26.7 nm, 520/27.9 nm
  Pos 3: Neutral Density — ND 1.3-5%T-25mm. 5% transmission to be used with widefield.

• Crest Dichroic Wheel
  Dichroic
  Element type: Filter Wheel
  Pos 1: Celesta-DA/FI/TR/Cy5/Cy7-A (MXR00543) — emission 441/36.1 nm, 511/34.3 nm, 592.5/45.2 nm, 684/44.4 nm, 817/78.8 nm — Explicit transmission windows are mapped. Reflection cutoffs: 421, 491, 567, 659, 776 nm.
  Pos 2: Celesta CFP-YFP-A (MXR00544) — emission 484/25.9 nm, 561/38.9 nm — Explicit transmission windows are mapped. Reflection cutoffs: 471, 539 nm.

• Crest Emission Wheel
  Emission
  Element type: Filter Wheel
  Pos 1: Celesta-DA/FI/TR/Cy5/Cy7-A (MXR00541 / MXR00543) — emission 441/36.1 nm, 511/34.3 nm, 592.5/45.2 nm, 684/44.4 nm, 817/78.8 nm
  Pos 2: Celesta CFP-YFP-A (MXR00542 / MXR00544) — emission 484/25.9 nm, 561/38.9 nm
  Pos 3: Bandpass — emission 438/24 nm
  Pos 4: Bandpass — emission 485/20 nm
  Pos 5: Bandpass — emission 511/20 nm
  Pos 6: Bandpass — emission 560/25 nm
  Pos 7: Bandpass — emission 595/31 nm
  Pos 8: Bandpass — emission 685/40 nm

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Latest QC measurements

Laser performance

No recent laser-performance measurements are recorded.

Optical resolution (PSF and alignment)

CFI Plan Apochromat Lambda S 60XC Sil DIC N2 60x/1.3 SILICONE (60x_silicone)

• PSF · 60x Silicone · 525 · FWHM XY Max nm

  ---

  288.3 nm
  psf.60x_silicone.525.fwhm_xy_max_nm

• PSF · 60x Silicone · 525 · FWHM XY Min nm

  ---

  267.1 nm
  psf.60x_silicone.525.fwhm_xy_min_nm

• PSF · 60x Silicone · 525 · FWHM Z nm

  ---

  853.2 nm
  psf.60x_silicone.525.fwhm_z_nm

• PSF · 60x Silicone · 525 · Asymmetry

  ---

  0.93
  psf.60x_silicone.525.asymmetry

• Chromatic Shift · 60x Silicone · 425 To 525 · Z nm

  ---

  127.5 nm
  chromatic_shift.60x_silicone.425_to_525.z_nm

CFI Plan Apochromat Lambda D 100x Oil 100x/1.45 OIL (100x_oil)

• PSF · 100x Oil · 525 · FWHM XY Max nm

  ---

  210 nm
  psf.100x_oil.525.fwhm_xy_max_nm

• PSF · 100x Oil · 525 · FWHM XY Min nm

  ---

  203.1 nm
  psf.100x_oil.525.fwhm_xy_min_nm

• PSF · 100x Oil · 525 · FWHM Z nm

  ---

  585.5 nm
  psf.100x_oil.525.fwhm_z_nm

• PSF · 100x Oil · 525 · Asymmetry

  ---

  0.97
  psf.100x_oil.525.asymmetry

• Chromatic Shift · 100x Oil · 425 To 525 · Z nm

  ---

  -25.4 nm
  chromatic_shift.100x_oil.425_to_525.z_nm

Objectives without recent measurements

• CFI Plan Apochromat Lambda D 4X 4x/0.2 AIR (4x_air)

• CFI Plan Apochromat Lambda D 20X DIC N2 20x/0.8 AIR (20x_air)

• CFI Plan Apochromat Lambda S 25XC Sil DIC N2 25x/1.05 SILICONE (25x_silicone)

• CFI Apochromat LWD Lambda S 40XC WI DIC N2 40x/1.15 WATER (40x_water)

• Apo TIRF 100x/1.49 Oil DIC N2 OIL (100x_oil_tirf)

Environment and stage performance

• Environment · Room Temperature C

  ---

  20 celsius
  environment.room_temperature_c

Information still to confirm

Some details in the facility record are incomplete. If any of these matter for your experiment, please check them with AIC Turku staff.

• Details needed for this configuration:

• Software version (software[].version)