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ONI Nanoimager

About this microscope

Here you’ll find the hardware and capabilities currently recorded for this microscope. If you are planning an experiment, AIC Turku staff can help with access, training, and configuration choices.

Current status: 🟢 Online

Operational

QC and maintenance history Open Virtual Microscope

ONI Nanoimager microscope
Manufacturer
ONI
Model
Nanoimager
Stand
Other
Eyepieces
Camera-only
Location
Turku Bioscience, BioCity, 5th floor, room 5149
Purchased
2022
Funding
—
Capabilities
These are the capabilities recorded for this microscope. Open the optical-route details below to see how the hardware is connected.
TIRFSMLMFRETSingle-particle trackingLive cell imagingSingle-molecule imagingSuper-resolution
Methods and the light path that records them
Each method below is explicitly associated in the instrument record with the physical light path that implements it. An imaging mode or contrast method that appears above but not here has no recorded method-to-path mapping yet.
  • Widefield fluorescence: TIRF, SMLM
Modules
Environmental Enclosure Piezo Z-Stage Hardware Autofocus


Software

  • NimOS microscope control software
    Acquisition
    Role: Acquisition
    Version: Not documented

  • CODI analysis software
    Processing
    Role: Processing
    Version: Not documented

Hardware

Environmental control

  • Environmental Control
    controlled temperature
    Temperature control: Yes
    Notes: Resistive heating without active cooling. Suitable for live cell imaging at 37°C.

Light sources

  • 405 nm Laser ONI 405 nm violet
    ONI
    Type: Laser
    Technology: diode
    Wavelength: 405 nm
    Power: 70 mW
    Notes: Power measured at the objective.

  • 488 nm Laser ONI 488 nm blue
    ONI
    Type: Laser
    Technology: diode
    Wavelength: 488 nm
    Power: 250 mW
    Notes: Power measured at the objective.

  • 561 nm Laser ONI 561 nm green
    ONI
    Type: Laser
    Technology: dpss
    Wavelength: 561 nm
    Power: 250 mW
    Notes: Power measured at the objective. DPSS technology inherently takes ~30 sec to stabilize.

  • 640 nm Laser ONI 640 nm red
    ONI
    Type: Laser
    Technology: diode
    Wavelength: 640 nm
    Power: 220 mW
    Notes: Power measured at the objective.

Detectors

  • Hamamatsu ORCA-Flash4.0 V3
    Scientific CMOS
    Type: Scientific CMOS
    Pixel pitch: 6.5 µm
    QE peak: 82%
    Notes: Digital sCMOS camera, 100 fps full frame. Typically operated with 2x2 binning.

Objectives

View this microscope's objectives in the catalogue

  • UPLXAPO100XO 100x/1.45 OIL
    Olympus
    Model: UPLXAPO100XO
    Magnification / NA: 100x/1.45
    Immersion: Oil
    Correction: Apochromat
    Working distance: 0.13 mm
    AFC compatible: Yes
    Installed: Yes

Magnification changers

  • Custom tube lens
    ONI
    Manufacturer: ONI
    Model: Custom tube lens
    Magnification: 1.1x
    Notes: Custom tube lens provides 110x total magnification with the 100x objective. Combined with 2x2 binning, this yields a pixel size of ~117 nm.

Stages and focus drives

  • Z Piezo Stage — ONI Closed-loop Piezo XYZ Stage
    ONI
    Type: Z Piezo Stage

Autofocus and triggering

  • Infrared Reflection Autofocus
    Installed: Yes
    Type: Infrared Reflection Autofocus

Latest QC measurements

Laser performance

No recent laser-performance measurements are recorded.

Optical resolution (PSF and alignment)

No recent PSF or chromatic-shift measurements are recorded for these objectives.

Objectives without recent measurements
  • UPLXAPO100XO 100x/1.45 OIL (100x_oil)

Information still to confirm

Some details in the facility record are incomplete. If any of these matter for your experiment, please check them with AIC Turku staff.

  • Details needed for this configuration:

  • Software version (software[].version)