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ONI Nanoimager

Work in progress

This dashboard is a work in progress. Please independently verify critical information, as not all features are working yet.

Current Status: 🟢 Online

Operational

📊 View QC & Maintenance History 🔬 Open Virtual Microscope

ONI Nanoimager
Manufacturer
ONI
Model
Nanoimager
Stand
Other
Eyepieces
Camera-only
Location
—
Purchased
2022
Funding
—
Modalities
Total Internal Reflection Fluorescence Single-Molecule Localization Microscopy Single-Particle Tracking Förster Resonance Energy Transfer
Modules
Environmental Enclosure Piezo Z-Stage Hardware Autofocus


Software Environment

  • NimOS microscope control software
    Acquisition
    Role: Acquisition

  • CODI analysis software
    Processing
    Role: Processing

Hardware Configuration

🌡️ Environmental Control

  • Environmental Control
    controlled temperature
    Temperature control: Yes
    Notes: Resistive heating without active cooling. Suitable for live cell imaging at 37°C.

💡 Light Sources

  • 405 nm Laser ONI 405 nm violet
    ONI
    Type: Laser
    Technology: diode
    Wavelength: 405 nm
    Power: 70 mW
    Notes: Power measured at the objective.

  • 488 nm Laser ONI 488 nm blue
    ONI
    Type: Laser
    Technology: diode
    Wavelength: 488 nm
    Power: 250 mW
    Notes: Power measured at the objective.

  • 561 nm Laser ONI 561 nm green
    ONI
    Type: Laser
    Technology: dpss
    Wavelength: 561 nm
    Power: 250 mW
    Notes: Power measured at the objective. DPSS technology inherently takes ~30 sec to stabilize.

  • 640 nm Laser ONI 640 nm red
    ONI
    Type: Laser
    Technology: diode
    Wavelength: 640 nm
    Power: 220 mW
    Notes: Power measured at the objective.

📷 Detectors

  • Hamamatsu ORCA-Flash4.0 V3
    Scientific CMOS
    Type: Scientific CMOS
    Pixel pitch: 6.5 µm
    QE peak: 82%
    Notes: Digital sCMOS camera, 100 fps full frame. Typically operated with 2x2 binning.

🔬 Objectives

  • UPLXAPO100XO 100x/1.45 OIL
    Olympus
    Model: UPLXAPO100XO
    Magnification / NA: 100x/1.45
    Immersion: Oil
    Correction: Apochromat
    Working distance: 0.13 mm
    AFC compatible: Yes
    Installed: Yes

🔍 Magnification Changers

  • Custom tube lens
    ONI
    Manufacturer: ONI
    Model: Custom tube lens
    Magnification: 1.1x
    Notes: Custom tube lens provides 110x total magnification with the 100x objective. Combined with 2x2 binning, this yields a pixel size of ~117 nm.

🧭 Stages & Focus Drives

  • Z Piezo Stage — ONI Closed-loop Piezo XYZ Stage
    ONI
    Type: Z Piezo Stage

⚙️ Advanced Hardware

  • Infrared Reflection Autofocus
    Installed: Yes
    Type: Infrared Reflection Autofocus

QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data
  • UPLXAPO100XO 100x/1.45 OIL (100x_oil)

Metadata Completeness

  • Missing required fields:

  • instrument.location — Location

  • Missing conditional fields (currently applicable):

  • software[].version — Software version

  • Legacy aliases in use (fallback accepted):

  • modules[].name → modules[].type

  • hardware.detectors[].pixel_size_um → hardware.detectors[].pixel_pitch_um

  • hardware.detectors[].min_nm → hardware.detectors[].collection_min_nm

  • hardware.detectors[].max_nm → hardware.detectors[].collection_max_nm

  • hardware.detectors[].channel_center_nm → hardware.detectors[].collection_center_nm

  • hardware.detectors[].bandwidth_nm → hardware.detectors[].collection_width_nm