Andor BC43 Benchtop Confocal
Work in progress
This dashboard is a work in progress. Please independently verify critical information, as not all features are working yet.
Software Environment
-
Fusion BC43 (v2.7.0)
Acquisition
Role: Acquisition
Developer: Andor
Version:2.7.0 -
Imaris Quant
Analysis
Role: Analysis
Developer: Oxford Instruments / Andor
Notes: Offline license for high-quality snapshots, movie creation, isosurface reconstructions, and traditional intensity-based/AI metric characterization.
Hardware Configuration
🌡️ Environmental Control
- Environmental Control
controlled temperature, controlled CO2, controlled humidity
Temperature control: Yes
CO2 control: Yes
Humidity control: Yes
Notes: TOPSTAGE-ANDOR-BL3 Incubator Bundle (T/H/CO2)
💡 Light Sources
-
405 nm Laser Andor Borealis Illumination
Andor
Type: Laser
Wavelength:405 nm
Notes: Part of BC43 excitation lines -
488 nm Laser Andor Borealis Illumination
Andor
Type: Laser
Wavelength:488 nm
Notes: Part of BC43 excitation lines -
561 nm Laser Andor Borealis Illumination
Andor
Type: Laser
Wavelength:561 nm
Notes: Part of BC43 excitation lines -
638 nm Laser Andor Borealis Illumination
Andor
Type: Laser
Wavelength:638 nm
Notes: Part of BC43 excitation lines -
broadband LED Andor Transmitted Light Illuminator
Andor
Type: LED
Role: Transmitted Illumination
Wavelength:broadband
Notes: Used for Brightfield and Differential Phase Contrast (DPC).
🧭 Scanner
- Spinning Disk Scanner
Type: Spinning Disk Scanner
Pinhole:50 µm
Notes: Microlens-spinning disk multipoint confocal. Features 50 µm pinholes with optimized spacing for deep tissue imaging.
📷 Detectors
- Andor 4.1 MP sCMOS
Scientific CMOS
Type: Scientific CMOS
Pixel pitch:6.5 µm
QE peak:82%
Notes: 16-bit. 18.4 mm field of view.
🔬 Objectives
-
10X Plan Apo LD Air 10x/0.45 AIR
Nikon
Model: 10X Plan Apo LD Air
Magnification / NA:10x/0.45
Immersion: Air
Correction: Plan Apochromat
Working distance:4.0 mm
Product code:INS-OBJ-10D-045
AFC compatible: Yes
Installed: Yes
Notes: Included in base BC43 CF. Optical family: Lambda D. -
20X Plan Apo LD Air 20x/0.8 AIR
Nikon
Model: 20X Plan Apo LD Air
Magnification / NA:20x/0.8
Immersion: Air
Correction: Plan Apochromat
Working distance:0.8 mm
Product code:INS-OBJ-20D-080
AFC compatible: Yes
Installed: Yes
Notes: Optical family: Lambda D. -
40X Plan Apo LD Air 40x/0.95 AIR
Nikon
Model: 40X Plan Apo LD Air
Magnification / NA:40x/0.95
Immersion: Air
Correction: Plan Apochromat
Working distance:0.21 mm
Product code:INS-OBJ-40D-095
AFC compatible: Yes
Installed: Yes
Notes: Optical family: Lambda D. -
40X Plan Apo LS Sil Oil 40x/1.25 SILICONE
Nikon
Model: 40X Plan Apo LS Sil Oil
Magnification / NA:40x/1.25
Immersion: Silicone
Correction: Plan Apochromat
Working distance:0.3 mm
Product code:INS-OBJ-40S-125-SIL
AFC compatible: Yes
Installed: Yes
Notes: Optical family: Lambda S. -
60X Plan Apo LD Oil 60x/1.42 OIL
Nikon
Model: 60X Plan Apo LD Oil
Magnification / NA:60x/1.42
Immersion: Oil
Correction: Plan Apochromat
Working distance:0.15 mm
Product code:INS-OBJ-60D-142-0
AFC compatible: Yes
Installed: Yes
Notes: Optical family: Lambda D. -
100x Plan Apo LD Oil 100x/1.45 OIL
Nikon
Model: 100x Plan Apo LD Oil
Magnification / NA:100x/1.45
Immersion: Oil
Correction: Plan Apochromat
Working distance:0.13 mm
Product code:INS-OBJ-100D-145-0
AFC compatible: Yes
Installed: Yes
Notes: Optical family: Lambda D.
⚙️ Advanced Hardware
- Infrared Reflection Autofocus
Installed: Yes
Type: Infrared Reflection Autofocus
🎛️ Optical Path Elements
-
BC43 Internal Dichroic
Dichroic
Element type: Fixed
Pos 1: Multiband Dichroic — emission 450/50 nm, 525/50 nm, 600/50 nm, 685/50 nm — Built-in multiband dichroic for standard 4-line excitation. Explicit transmission windows are aligned to the installed emission channels. -
BC43 Internal Emission Filters
Emission
Element type: Filter Wheel
Pos 1: DAPI — emission 450/50 nm
Pos 2: GFP — emission 525/50 nm
Pos 3: mCherry — emission 600/50 nm
Pos 4: CY5 — emission 685/50 nm
QC Metrics: Latest Measurements
🔦 Laser Performance
-
laser.at_obj.405.100pct.power_mw
18.0
laser.at_obj.405.100pct.power_mw -
laser.at_obj.488.100pct.power_mw
12.0
laser.at_obj.488.100pct.power_mw -
laser.at_obj.561.100pct.power_mw
13.0
laser.at_obj.561.100pct.power_mw -
laser.at_obj.638.100pct.power_mw
16.0
laser.at_obj.638.100pct.power_mw
🔬 Optical Resolution (PSF & Alignment)
No recent PSF or chromatic shift metrics available for any objectives.
Objectives without recent data
-
10X Plan Apo LD Air 10x/0.45 AIR (
10x_air) -
20X Plan Apo LD Air 20x/0.8 AIR (
20x_air) -
40X Plan Apo LD Air 40x/0.95 AIR (
40x_air) -
40X Plan Apo LS Sil Oil 40x/1.25 SILICONE (
40x_silicone) -
60X Plan Apo LD Oil 60x/1.42 OIL (
60x_oil) -
100x Plan Apo LD Oil 100x/1.45 OIL (
100x_oil)
Metadata Completeness
-
Missing conditional fields (currently applicable):
-
software[].version— Software version -
Legacy aliases in use (fallback accepted):
-
modules[].name→modules[].type -
hardware.detectors[].pixel_size_um→hardware.detectors[].pixel_pitch_um -
hardware.detectors[].min_nm→hardware.detectors[].collection_min_nm -
hardware.detectors[].max_nm→hardware.detectors[].collection_max_nm -
hardware.detectors[].channel_center_nm→hardware.detectors[].collection_center_nm -
hardware.detectors[].bandwidth_nm→hardware.detectors[].collection_width_nm