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3i Marianas CSU-W1 Spinning Disk Med C

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Current Status: 🟢 Online

Operational

📊 View QC & Maintenance History 🔬 Open Virtual Microscope

3i Marianas CSU-W1 Spinning Disk Med C
Manufacturer
3i / Zeiss
Model
Marianas CSU-W1 Spinning Disk Confocal
Stand
Inverted
Eyepieces
Trinocular
Location
Medisiina C, 2nd floor, room C242
Purchased
2019
Funding
—
Modalities
Spinning-Disk Confocal Widefield Fluorescence Transmitted Brightfield
Modules
Incubation Hardware Autofocus Motorized Stage Piezo Z-Stage


Software Environment

  • SlideBook (v6)
    Acquisition
    Role: Acquisition
    Developer: 3i
    Version: 6
    Notes: SlideBook 6 Full Online License with hardware control and TTL Synchronization.

Hardware Configuration

🌡️ Environmental Control

  • Environmental Control
    controlled temperature, controlled CO2, controlled O2
    Temperature control: Yes
    CO2 control: Yes
    O2 control: Yes
    Notes: Okolab full enclosure with Bold Line temperature controller, heater, and digital CO2/O2 gas mixer.

💡 Light Sources

  • 405 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 405 nm
    Power: 100 mW
    Notes: Solid state laser. Measured fiber output: 46 mW; Back Aperture Output: 3.7 mW.

  • 488 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 488 nm
    Power: 150 mW
    Notes: Solid state laser. Measured fiber output: 102 mW; Back Aperture Output: 9.6 mW.

  • 561 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 561 nm
    Power: 150 mW
    Notes: Solid state laser. Measured fiber output: 105.5 mW; Back Aperture Output: 10.5 mW.

  • 640 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 640 nm
    Power: 100 mW
    Notes: Solid state laser. Measured fiber output: 50.4 mW; Back Aperture Output: 6.3 mW.

  • LED CoolLED pE-300
    CoolLED
    Type: LED
    Notes: CoolLED PE-300 White Light Source with 3mm Liquid Light Guide. Broadband illumination.

🧭 Scanner

  • Spinning Disk Scanner
    Type: Spinning Disk Scanner
    Pinhole: 50 µm
    Notes: Yokogawa CSU-W1 T1 Spinning Disk Confocal with 50µm disk.

📷 Detectors

  • Photometrics Prime BSI
    Scientific CMOS
    Type: Scientific CMOS
    Pixel pitch: 6.5 µm
    Sensor format: 2048 x 2048
    QE peak: 95%
    Notes: 4 Megapixel 95% QE backside illuminated sensor, 1.3e- read noise. Hardware specifications sourced from test report. Can also be used for widefield fluorescence imaging.

🔬 Objectives

  • EC Plan-Neofluar 5x/0.16 NA Ph1 5x/0.16 AIR
    Zeiss
    Model: EC Plan-Neofluar 5x/0.16 NA Ph1
    Magnification / NA: 5x/0.16
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 18.5 mm
    Product code: 420331-9911-000
    AFC compatible: No
    Installed: Yes
    Specialties: phase
    Notes: Ph1. Without immersion.

  • Plan-Apochromat 10x/0.45 NA Ph1 10x/0.45 AIR
    Zeiss
    Model: Plan-Apochromat 10x/0.45 NA Ph1
    Magnification / NA: 10x/0.45
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 2.0 mm
    Product code: 420641-9910-000
    AFC compatible: No
    Installed: Yes
    Specialties: phase
    Notes: Ph1. Without immersion.

  • Plan-Apochromat 20x/0.8 NA 20x/0.8 AIR
    Zeiss
    Model: Plan-Apochromat 20x/0.8 NA
    Magnification / NA: 20x/0.8
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 0.55 mm
    Product code: 420650-9902-000
    AFC compatible: Yes
    Installed: Yes
    Notes: Without immersion.

  • LD Plan-Neofluar 20x/0.4 NA Corr 20x/0.4 AIR
    Zeiss
    Model: LD Plan-Neofluar 20x/0.4 NA Corr
    Magnification / NA: 20x/0.4
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 7.9 mm
    Product code: 421350-9971-000
    AFC compatible: Yes
    Installed: Yes
    Specialties: correction_collar
    Notes: Correction collar for 0-1.5 mm glass. Without immersion.

  • LD Plan-Neofluar 40x/0.6 NA Corr 40x/0.6 AIR
    Zeiss
    Model: LD Plan-Neofluar 40x/0.6 NA Corr
    Magnification / NA: 40x/0.6
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 2.9 mm
    Product code: 421360-9971-000
    AFC compatible: Yes
    Installed: Yes
    Specialties: correction_collar
    Notes: Correction collar for 0-1.5 mm glass. Without immersion.

  • C-Apochromat 40x/1.2 W Corr 40x/1.2 WATER
    Zeiss
    Model: C-Apochromat 40x/1.2 W Corr
    Magnification / NA: 40x/1.2
    Immersion: Water
    Correction: Apochromat
    Working distance: 0.28 mm
    Product code: 421767-9970-000
    AFC compatible: Yes
    Installed: Yes
    Specialties: correction_collar
    Notes: Correction ring for 0.17 mm cover glass. Immersion: Water.

  • Plan-Apochromat 63x/1.4 NA Oil 63x/1.4 OIL
    Zeiss
    Model: Plan-Apochromat 63x/1.4 NA Oil
    Magnification / NA: 63x/1.4
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.19 mm
    Product code: 420780-9900-000
    AFC compatible: Yes
    Installed: Yes
    Notes: Immersion: Oil.

  • alpha Plan-Apochromat 100x/1.46 NA Oil DIC 100x/1.46 OIL
    Zeiss
    Model: alpha Plan-Apochromat 100x/1.46 NA Oil DIC
    Magnification / NA: 100x/1.46
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.11 mm
    Product code: 420792-9800-000
    AFC compatible: Yes
    Installed: Yes
    Specialties: dic
    Notes: Immersion: Oil.

🧭 Stages & Focus Drives

  • Motorized XY Stage — ASI
    ASI
    Type: Motorized XY Stage

  • Z Piezo Stage — ASI
    ASI
    Type: Z Piezo Stage

⚙️ Advanced Hardware

  • Infrared Reflection Autofocus
    Installed: Yes
    Type: Infrared Reflection Autofocus

🎛️ Optical Path Elements

  • Zeiss Widefield Fluorescence Positions
    Cube
    Element type: Turret
    Pos 1: LED-DAPI-A-ZHE-Zero — emission 445/45 nm — DAPI Filter Cube. Nominal DAPI band 445/45nm.
    Pos 2: LED-FITC-A-ZHE-Zero — emission 525/30 nm — FITC Filter Cube. Nominal GFP band 525/30nm.
    Pos 3: LED-TRITC-A-ZHE-Zero — emission 617/73 nm — TRITC Filter Cube. Nominal Cy3/Alexa 568 band 617/73nm.

  • CSU-W1 Dichroic Slider
    Dichroic
    Element type: Slider
    Pos 1: Multiband Dichroic (Di01-T405/488/568/647-13x15x0.5) — emission 440/25 nm, 521/25 nm, 607/25 nm, 700/25 nm — Quad band Dichroic for CSU-W1 405/488/568/647 Excitation.

  • CSU-W1 Emission Wheel
    Emission
    Element type: Filter Wheel
    Pos 1: Multiband Bandpass (FF01-440/521/607/700-25) — emission 440/25 nm, 521/21 nm, 607/34 nm, 700/45 nm — Quad Band Emitter.
    Pos 2: Bandpass (FF01-445/45-25) — emission 445/45 nm — Individual Emitter.
    Pos 3: Bandpass (FF01-525/30-25) — emission 525/30 nm — Individual Emitter.
    Pos 4: Bandpass (FF02-617/73-25) — emission 617/73 nm — Individual Emitter.
    Pos 5: Bandpass (FF01-692/40-25) — emission 692/40 nm — Individual Emitter.

QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data

  • EC Plan-Neofluar 5x/0.16 NA Ph1 5x/0.16 AIR (5x_air)

  • Plan-Apochromat 10x/0.45 NA Ph1 10x/0.45 AIR (10x_air)

  • Plan-Apochromat 20x/0.8 NA 20x/0.8 AIR (20x_air)

  • LD Plan-Neofluar 20x/0.4 NA Corr 20x/0.4 AIR (20x_air_2)

  • LD Plan-Neofluar 40x/0.6 NA Corr 40x/0.6 AIR (40x_air)

  • C-Apochromat 40x/1.2 W Corr 40x/1.2 WATER (40x_water)

  • Plan-Apochromat 63x/1.4 NA Oil 63x/1.4 OIL (63x_oil)

  • alpha Plan-Apochromat 100x/1.46 NA Oil DIC 100x/1.46 OIL (100x_oil)

Metadata Completeness

  • Missing conditional fields (currently applicable):

  • modules[].model — Module model

  • Legacy aliases in use (fallback accepted):

  • modules[].name → modules[].type

  • hardware.detectors[].pixel_size_um → hardware.detectors[].pixel_pitch_um

  • hardware.detectors[].min_nm → hardware.detectors[].collection_min_nm

  • hardware.detectors[].max_nm → hardware.detectors[].collection_max_nm

  • hardware.detectors[].channel_center_nm → hardware.detectors[].collection_center_nm

  • hardware.detectors[].bandwidth_nm → hardware.detectors[].collection_width_nm