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3i Marianas CSU-W1 Spinning Disk Med C

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Current Status: 🟢 Online

Operational

📊 View QC & Maintenance History 🔬 Open Virtual Microscope

3i Marianas CSU-W1 Spinning Disk Med C
Manufacturer
3i / Zeiss
Model
Marianas CSU-W1 Spinning Disk Confocal
Stand
Inverted
Eyepieces
Trinocular
Location
Medisiina C, 2nd floor, room C242
Purchased
2019
Funding
—
Capabilities
Use these chips to quickly decide if this microscope supports your planned imaging mode, contrast method, or readout.
Confocal spinning diskWidefield fluorescenceTransmitted brightfieldPhase contrastDICLive cell imaging
Modules
Incubation Hardware Autofocus Motorized Stage Piezo Z-Stage


Software Environment

  • SlideBook (v6)
    Acquisition
    Role: Acquisition
    Developer: 3i
    Version: 6
    Notes: SlideBook 6 Full Online License with hardware control and TTL Synchronization.

Hardware Configuration

🌡️ Environmental Control

  • Environmental Control
    controlled temperature, controlled CO2, controlled O2
    Temperature control: Yes
    CO2 control: Yes
    O2 control: Yes
    Notes: Okolab full enclosure with Bold Line temperature controller, heater, and digital CO2/O2 gas mixer.

💡 Light Sources

  • 405 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 405 nm
    Power: 100 mW
    Notes: Solid state laser. Measured fiber output: 46 mW; Back Aperture Output: 3.7 mW.

  • 488 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 488 nm
    Power: 150 mW
    Notes: Solid state laser. Measured fiber output: 102 mW; Back Aperture Output: 9.6 mW.

  • 561 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 561 nm
    Power: 150 mW
    Notes: Solid state laser. Measured fiber output: 105.5 mW; Back Aperture Output: 10.5 mW.

  • 640 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 640 nm
    Power: 100 mW
    Notes: Solid state laser. Measured fiber output: 50.4 mW; Back Aperture Output: 6.3 mW.

  • LED CoolLED pE-300
    CoolLED
    Type: LED
    Notes: CoolLED PE-300 White Light Source with 3mm Liquid Light Guide. Broadband illumination.

🧭 Scanner

  • Spinning Disk Scanner
    Type: Spinning Disk Scanner
    Pinhole: 50 µm
    Notes: Yokogawa CSU-W1 T1 Spinning Disk Confocal with 50µm disk.

📷 Detectors

  • Photometrics Prime BSI
    Scientific CMOS
    Type: Scientific CMOS
    Pixel pitch: 6.5 µm
    Sensor format: 2048 x 2048
    QE peak: 95%
    Notes: 4 Megapixel 95% QE backside illuminated sensor, 1.3e- read noise. Hardware specifications sourced from test report. Can also be used for widefield fluorescence imaging.

🔬 Objectives

  • EC Plan-Neofluar 5x/0.16 NA Ph1 5x/0.16 AIR
    Zeiss
    Model: EC Plan-Neofluar 5x/0.16 NA Ph1
    Magnification / NA: 5x/0.16
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 18.5 mm
    Product code: 420331-9911-000
    AFC compatible: No
    Installed: Yes
    Specialties: phase
    Notes: Ph1. Without immersion.

  • Plan-Apochromat 10x/0.45 NA Ph1 10x/0.45 AIR
    Zeiss
    Model: Plan-Apochromat 10x/0.45 NA Ph1
    Magnification / NA: 10x/0.45
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 2.0 mm
    Product code: 420641-9910-000
    AFC compatible: No
    Installed: Yes
    Specialties: phase
    Notes: Ph1. Without immersion.

  • Plan-Apochromat 20x/0.8 NA 20x/0.8 AIR
    Zeiss
    Model: Plan-Apochromat 20x/0.8 NA
    Magnification / NA: 20x/0.8
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 0.55 mm
    Product code: 420650-9902-000
    AFC compatible: Yes
    Installed: Yes
    Notes: Without immersion.

  • LD Plan-Neofluar 20x/0.4 NA Corr 20x/0.4 AIR
    Zeiss
    Model: LD Plan-Neofluar 20x/0.4 NA Corr
    Magnification / NA: 20x/0.4
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 7.9 mm
    Product code: 421350-9971-000
    AFC compatible: Yes
    Installed: Yes
    Specialties: correction_collar
    Notes: Correction collar for 0-1.5 mm glass. Without immersion.

  • LD Plan-Neofluar 40x/0.6 NA Corr 40x/0.6 AIR
    Zeiss
    Model: LD Plan-Neofluar 40x/0.6 NA Corr
    Magnification / NA: 40x/0.6
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 2.9 mm
    Product code: 421360-9971-000
    AFC compatible: Yes
    Installed: Yes
    Specialties: correction_collar
    Notes: Correction collar for 0-1.5 mm glass. Without immersion.

  • C-Apochromat 40x/1.2 W Corr 40x/1.2 WATER
    Zeiss
    Model: C-Apochromat 40x/1.2 W Corr
    Magnification / NA: 40x/1.2
    Immersion: Water
    Correction: Apochromat
    Working distance: 0.28 mm
    Product code: 421767-9970-000
    AFC compatible: Yes
    Installed: Yes
    Specialties: correction_collar
    Notes: Correction ring for 0.17 mm cover glass. Immersion: Water.

  • Plan-Apochromat 63x/1.4 NA Oil 63x/1.4 OIL
    Zeiss
    Model: Plan-Apochromat 63x/1.4 NA Oil
    Magnification / NA: 63x/1.4
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.19 mm
    Product code: 420780-9900-000
    AFC compatible: Yes
    Installed: Yes
    Notes: Immersion: Oil.

  • alpha Plan-Apochromat 100x/1.46 NA Oil DIC 100x/1.46 OIL
    Zeiss
    Model: alpha Plan-Apochromat 100x/1.46 NA Oil DIC
    Magnification / NA: 100x/1.46
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.11 mm
    Product code: 420792-9800-000
    AFC compatible: Yes
    Installed: Yes
    Specialties: dic
    Notes: Immersion: Oil.

🧭 Stages & Focus Drives

  • Motorized XY Stage — ASI
    ASI
    Type: Motorized XY Stage

  • Z Piezo Stage — ASI
    ASI
    Type: Z Piezo Stage

⚙️ Advanced Hardware

  • Infrared Reflection Autofocus
    Installed: Yes
    Type: Infrared Reflection Autofocus

🎛️ Optical Path Elements

  • Zeiss Widefield Fluorescence Positions
    Cube
    Element type: Turret
    Pos 1: LED-DAPI-A-ZHE-Zero — emission 445/45 nm — DAPI Filter Cube. Nominal DAPI band 445/45nm.
    Pos 2: LED-FITC-A-ZHE-Zero — emission 525/30 nm — FITC Filter Cube. Nominal GFP band 525/30nm.
    Pos 3: LED-TRITC-A-ZHE-Zero — emission 617/73 nm — TRITC Filter Cube. Nominal Cy3/Alexa 568 band 617/73nm.

  • CSU-W1 Dichroic Slider
    Dichroic
    Element type: Slider
    Pos 1: Multiband Dichroic (Di01-T405/488/568/647-13x15x0.5) — emission 440/25 nm, 521/25 nm, 607/25 nm, 700/25 nm — Quad band Dichroic for CSU-W1 405/488/568/647 Excitation.

  • CSU-W1 Emission Wheel
    Emission
    Element type: Filter Wheel
    Pos 1: Multiband Bandpass (FF01-440/521/607/700-25) — emission 440/25 nm, 521/21 nm, 607/34 nm, 700/45 nm — Quad Band Emitter.
    Pos 2: Bandpass (FF01-445/45-25) — emission 445/45 nm — Individual Emitter.
    Pos 3: Bandpass (FF01-525/30-25) — emission 525/30 nm — Individual Emitter.
    Pos 4: Bandpass (FF02-617/73-25) — emission 617/73 nm — Individual Emitter.
    Pos 5: Bandpass (FF01-692/40-25) — emission 692/40 nm — Individual Emitter.

QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data

  • EC Plan-Neofluar 5x/0.16 NA Ph1 5x/0.16 AIR (5x_air)

  • Plan-Apochromat 10x/0.45 NA Ph1 10x/0.45 AIR (10x_air)

  • Plan-Apochromat 20x/0.8 NA 20x/0.8 AIR (20x_air)

  • LD Plan-Neofluar 20x/0.4 NA Corr 20x/0.4 AIR (20x_air_2)

  • LD Plan-Neofluar 40x/0.6 NA Corr 40x/0.6 AIR (40x_air)

  • C-Apochromat 40x/1.2 W Corr 40x/1.2 WATER (40x_water)

  • Plan-Apochromat 63x/1.4 NA Oil 63x/1.4 OIL (63x_oil)

  • alpha Plan-Apochromat 100x/1.46 NA Oil DIC 100x/1.46 OIL (100x_oil)

Metadata Completeness

  • Missing conditional fields (currently applicable):

  • modules[].model — Module model