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3i Marianas CSU-W1 Spinning Disk Med C

About this microscope

Here you’ll find the hardware and capabilities currently recorded for this microscope. If you are planning an experiment, AIC Turku staff can help with access, training, and configuration choices.

Current status: 🟢 Online

Operational

QC and maintenance history Open Virtual Microscope

3i Marianas CSU-W1 Spinning Disk Med C microscope
Manufacturer
3i / Zeiss
Model
Marianas CSU-W1 Spinning Disk Confocal
Stand
Inverted
Eyepieces
Trinocular
Location
Medisiina C, 2nd floor, room C242
Purchased
2019
Funding
—
Capabilities
These are the capabilities recorded for this microscope. Open the optical-route details below to see how the hardware is connected.
Confocal spinning diskWidefield fluorescenceTransmitted brightfieldPhase contrastDICLive cell imaging
Methods and the light path that records them
Each method below is explicitly associated in the instrument record with the physical light path that implements it. An imaging mode or contrast method that appears above but not here has no recorded method-to-path mapping yet.
  • Spinning-disk confocal: Confocal spinning disk
  • Widefield fluorescence: Widefield fluorescence
  • Transmitted light: Transmitted brightfield, Phase contrast, DIC
Modules
Incubation Hardware Autofocus Motorized Stage Piezo Z-Stage


Software

  • SlideBook (v6)
    Acquisition
    Role: Acquisition
    Developer: 3i
    Version: 6
    Notes: SlideBook 6 Full Online License with hardware control and TTL Synchronization.

Hardware

Environmental control

  • Environmental Control
    controlled temperature, controlled CO2, controlled O2
    Temperature control: Yes
    CO2 control: Yes
    O2 control: Yes
    Notes: Okolab full enclosure with Bold Line temperature controller, heater, and digital CO2/O2 gas mixer.

Light sources

  • 405 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 405 nm
    Power: 100 mW
    Notes: Solid state laser. Measured fiber output: 46 mW; Back Aperture Output: 3.7 mW. SlideBook confocal configurations select Alternate Source position 1 (Laser = 8) for 405 nm.

  • 488 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 488 nm
    Power: 150 mW
    Notes: Solid state laser. Measured fiber output: 102 mW; Back Aperture Output: 9.6 mW. SlideBook confocal configurations select Alternate Source position 3 (Laser = 32) for 488 nm.

  • 561 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 561 nm
    Power: 150 mW
    Notes: Solid state laser. Measured fiber output: 105.5 mW; Back Aperture Output: 10.5 mW. SlideBook confocal configurations select Alternate Source position 5 (Laser = 128) for 561 nm.

  • 640 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 640 nm
    Power: 100 mW
    Notes: Solid state laser. Measured fiber output: 50.4 mW; Back Aperture Output: 6.3 mW. SlideBook confocal configurations select Alternate Source position 6 (Laser = 2) for 640 nm.

  • LED CoolLED pE-300
    CoolLED
    Type: LED
    Role: Excitation
    Notes: CoolLED pE-300 white-light LED source with 3 mm liquid light guide. Installed for widefield fluorescence excitation. The transmitted-light source used for brightfield/phase/DIC is not identified by the available records.

Scanner

  • Spinning Disk Scanner
    Yokogawa CSU-W1 T1
    Type: Spinning Disk Scanner
    Manufacturer: Yokogawa
    Model: CSU-W1 T1
    Pinhole: 50 µm
    Notes: Yokogawa CSU-W1 T1 Spinning Disk Confocal with 50µm disk.

Detectors

  • Photometrics Prime BSI
    Scientific CMOS
    Type: Scientific CMOS
    Pixel pitch: 6.5 µm
    Sensor format: 2048 x 2048
    QE peak: 95%
    Notes: 4 Megapixel 95% QE backside illuminated sensor, 1.3e- read noise. Hardware specifications sourced from the 2019 system test report. SlideBook filter-configuration screenshots identify this as Camera 1 (PMUSBCam00). Can also be used for widefield fluorescence and transmitted-light imaging.

Objectives

View this microscope's objectives in the catalogue

  • EC Plan-Neofluar 5x/0.16 NA Ph1 AIR
    Zeiss
    Model: EC Plan-Neofluar 5x/0.16 NA Ph1
    Magnification / NA: 5x/0.16
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 18.5 mm
    Product code: 420331-9911-000
    AFC compatible: No
    Installed: Yes
    Specialties: phase
    Notes: Ph1. Without immersion.

  • Plan-Apochromat 10x/0.45 NA Ph1 AIR
    Zeiss
    Model: Plan-Apochromat 10x/0.45 NA Ph1
    Magnification / NA: 10x/0.45
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 2.0 mm
    Product code: 420641-9910-000
    AFC compatible: No
    Installed: Yes
    Specialties: phase
    Notes: Ph1. Without immersion.

  • Plan-Apochromat 20x/0.8 NA AIR
    Zeiss
    Model: Plan-Apochromat 20x/0.8 NA
    Magnification / NA: 20x/0.8
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 0.55 mm
    Product code: 420650-9902-000
    AFC compatible: Yes
    Installed: Yes
    Notes: Without immersion.

  • LD Plan-Neofluar 20x/0.4 NA Corr AIR
    Zeiss
    Model: LD Plan-Neofluar 20x/0.4 NA Corr
    Magnification / NA: 20x/0.4
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 7.9 mm
    Product code: 421350-9971-000
    AFC compatible: Yes
    Installed: Yes
    Specialties: correction_collar
    Notes: Correction collar for 0-1.5 mm glass. Without immersion.

  • LD Plan-Neofluar 40x/0.6 NA Corr AIR
    Zeiss
    Model: LD Plan-Neofluar 40x/0.6 NA Corr
    Magnification / NA: 40x/0.6
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 2.9 mm
    Product code: 421360-9971-000
    AFC compatible: Yes
    Installed: Yes
    Specialties: correction_collar
    Notes: Correction collar for 0-1.5 mm glass. Without immersion.

  • C-Apochromat 40x/1.2 W Corr WATER
    Zeiss
    Model: C-Apochromat 40x/1.2 W Corr
    Magnification / NA: 40x/1.2
    Immersion: Water
    Correction: Apochromat
    Working distance: 0.28 mm
    Product code: 421767-9970-000
    AFC compatible: Yes
    Installed: Yes
    Specialties: correction_collar
    Notes: Correction ring for 0.17 mm cover glass. Immersion: Water.

  • Plan-Apochromat 63x/1.4 NA Oil OIL
    Zeiss
    Model: Plan-Apochromat 63x/1.4 NA Oil
    Magnification / NA: 63x/1.4
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.19 mm
    Product code: 420780-9900-000
    AFC compatible: Yes
    Installed: Yes
    Notes: Immersion: Oil.

  • alpha Plan-Apochromat 100x/1.46 NA Oil DIC OIL
    Zeiss
    Model: alpha Plan-Apochromat 100x/1.46 NA Oil DIC
    Magnification / NA: 100x/1.46
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.11 mm
    Product code: 420792-9800-000
    AFC compatible: Yes
    Installed: Yes
    Specialties: dic
    Notes: Immersion: Oil.

Stages and focus drives

  • Motorized XY Stage — ASI
    ASI
    Type: Motorized XY Stage

  • Z Piezo Stage — ASI
    ASI
    Type: Z Piezo Stage

Autofocus and triggering

  • Infrared Reflection Autofocus
    Installed: Yes
    Type: Infrared Reflection Autofocus

Optical-path elements

  • Zeiss Widefield Fluorescence Positions
    Cube
    Element type: Turret
    Pos 1: Filter Cube (LED-DAPI-A-ZHE-Zero) — LED-optimized DAPI filter set in a Zeiss cube. Spectral prescription (392/23 excitation, 409 nm dichroic edge, 447/60 emission) from the Semrock BrightLine LED-DAPI-A set, IDEX Health & Science LED filter-set datasheet (2016): https://www.idex-hs.com/docs/default-source/data-sheets/idx-smk-leddatasheet-web.pdf. The ZHE-ZERO suffix specifies the Zeiss cube/zero-pixel-shift implementation. Nominal catalogue specification, not a measured transmission curve.
    Pos 2: Filter Cube (LED-FITC-A-ZHE-Zero) — LED-optimized FITC filter set in a Zeiss cube. Spectral prescription (474/27 excitation, 495 nm dichroic edge, 525/45 emission) from the Semrock BrightLine LED-FITC-A set, IDEX Health & Science LED filter-set datasheet (2016): https://www.idex-hs.com/docs/default-source/data-sheets/idx-smk-leddatasheet-web.pdf. The ZHE-ZERO suffix specifies the Zeiss cube/zero-pixel-shift implementation. Nominal catalogue specification, not a measured transmission curve.
    Pos 3: Filter Cube (LED-TRITC-A-ZHE-Zero) — LED-optimized TRITC filter set in a Zeiss cube. Spectral prescription (554/23 excitation, 573 nm dichroic edge, 609/54 emission) from the Semrock BrightLine LED-TRITC-A set, IDEX Health & Science LED filter-set datasheet (2016): https://www.idex-hs.com/docs/default-source/data-sheets/idx-smk-leddatasheet-web.pdf. The ZHE-ZERO suffix specifies the Zeiss cube/zero-pixel-shift implementation. Nominal catalogue specification, not a measured transmission curve.

  • Yokogawa CSU-W Filter Turret
    Dichroic
    Element type: Turret
    Pos 1: Multiband Dichroic (Di01-T405/488/568/647-13x15x0.5) — Quad-band confocal dichroic documented in the 2019 system test report. SlideBook confocal configurations select CSU-W Filter Turret position 1. No generic pass-band array is authored because the dichroic alternates transmission and reflection bands.
    Pos 2: Empty — Documented as empty in the 2019 system test report.
    Pos 3: Empty — Documented as empty in the 2019 system test report.

  • Yokogawa CSU-W Emission Filter Wheel 1
    Emission
    Element type: Filter Wheel
    Details: The 2019 system test report documents positions 1-6; current SlideBook configurations additionally demonstrate position 8. Position 7 remains undocumented rather than being treated as empty.
    Pos 1: Quad-band emitter (FF01-440/521/607/700-25) — emission 440/40 nm, 521/21 nm, 607/34 nm, 700/45 nm — Quad-band emitter. SlideBook c405q, c488q, c561q and c640q configurations all select filter-wheel position 1.
    Pos 2: DAPI (FF01-445/45-25) — emission 445/45 nm — Individual emitter. SlideBook c405 selects filter-wheel position 2.
    Pos 3: GFP (FF01-525/30-25) — emission 525/30 nm — Individual emitter. SlideBook c488 selects filter-wheel position 3.
    Pos 4: Cy3 / Alexa 568 (FF02-617/73-25) — emission 617/73 nm — Individual emitter. SlideBook c561 selects filter-wheel position 4.
    Pos 5: Cy5 / Alexa 647 (FF01-692/40-25) — emission 692/40 nm — Individual emitter documented in the 2019 system test report; the corresponding c640 single-band SlideBook configuration was not included in the supplied screenshots.
    Pos 6: Empty — Documented as empty in the 2019 system test report.
    Pos 8: 600/37 — emission 600/37 nm — SlideBook configuration named 600/37 selects the 561 nm source and CSU-W Filter Wheel 1 position 8. Manufacturer and product code are not identified by the available evidence.

Latest QC measurements

Laser performance

No recent laser-performance measurements are recorded.

Optical resolution (PSF and alignment)

No recent PSF or chromatic-shift measurements are recorded for these objectives.

Objectives without recent measurements

  • EC Plan-Neofluar 5x/0.16 NA Ph1 AIR (5x_air)

  • Plan-Apochromat 10x/0.45 NA Ph1 AIR (10x_air)

  • Plan-Apochromat 20x/0.8 NA AIR (20x_air)

  • LD Plan-Neofluar 20x/0.4 NA Corr AIR (20x_air_2)

  • LD Plan-Neofluar 40x/0.6 NA Corr AIR (40x_air)

  • C-Apochromat 40x/1.2 W Corr WATER (40x_water)

  • Plan-Apochromat 63x/1.4 NA Oil OIL (63x_oil)

  • alpha Plan-Apochromat 100x/1.46 NA Oil DIC OIL (100x_oil)

Information still to confirm

Some details in the facility record are incomplete. If any of these matter for your experiment, please check them with AIC Turku staff.

  • Details needed for this configuration:

  • Module model (modules[].model)