Abberior STED

About this microscope

Here you’ll find the hardware and capabilities currently recorded for this microscope. If you are planning an experiment, AIC Turku staff can help with access, training, and configuration choices.

Current status: 🟢 Online

Operational

QC and maintenance history Open Virtual Microscope

Manufacturer

Abberior

Model

STED/RESOLFT

Stand

Inverted

Eyepieces

Trinocular

Location

BioCity, 5th floor, BioPhysics room 5086

Purchased

—

Funding

—

Capabilities

These are the capabilities recorded for this microscope. Open the optical-route details below to see how the hardware is connected.

STEDRESOLFTConfocal point scanningSuper-resolutionLive cell imaging

Methods and the light path that records them

Each method below is explicitly associated in the instrument record with the physical light path that implements it. An imaging mode or contrast method that appears above but not here has no recorded method-to-path mapping yet.

• Point-scanning confocal: Confocal point scanning, STED, RESOLFT

Modules

Easy3D STED Adaptive Optics RESCue STED

Software

• Abberior Imspector
  Acquisition
  Role: Acquisition
  Version: Not documented

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Hardware

Light sources

• 485 nm Laser PicoQuant LDH-D-C-485
  PicoQuant
  Type: Laser
  Role: Excitation
  Timing mode: Pulsed
  Pulse width: 80 ps
  Repetition rate: 40 MHz
  Wavelength: 485 nm
  Notes: Pulsed excitation source for the 488 nm channel.

• 561 nm Laser PicoQuant PDL-T 561
  PicoQuant
  Type: Laser
  Role: Excitation
  Timing mode: Pulsed
  Pulse width: 80 ps
  Repetition rate: 40 MHz
  Wavelength: 561 nm
  Notes: Pulsed excitation source for the 561 nm channel.

• 640 nm Laser PicoQuant LDH-D-C-640
  PicoQuant
  Type: Laser
  Role: Excitation
  Timing mode: Pulsed
  Pulse width: 80 ps
  Repetition rate: 40 MHz
  Wavelength: 640 nm
  Notes: Pulsed excitation source for the 640 nm channel.

• 580 nm Laser MPB Communications VFL-P-1000-580
  MPB Communications
  Type: Laser
  Role: Depletion
  Timing mode: Continuous Wave
  Depletion targets: 488 nm
  Wavelength: 580 nm
  Notes: Continuous-wave STED depletion source for the 488 nm excitation channel.

• 775 nm Laser OneFive / NKT Photonics Katana HP 775
  OneFive / NKT Photonics
  Type: Laser
  Role: Depletion
  Timing mode: Pulsed
  Pulse width: 600 ps
  Repetition rate: 40 MHz
  Depletion targets: 561 nm, 640 nm
  Wavelength: 775 nm
  Notes: Pulsed STED depletion source for the 561 nm and 640 nm excitation channels.

Scanner

• Galvanometric Scanner
  Type: Galvanometric Scanner
  Notes: STED point scanning configuration.

Detectors

• Hamamatsu Photonics Photomultiplier Tube
  Photomultiplier Tube
  Type: Photomultiplier Tube
  Notes: General confocal detection channel.

• Excelitas Technologies SPCM-AQRH-14-TR
  Avalanche Photodiode
  Type: Avalanche Photodiode
  Supports time gating: Yes
  Default gating delay: 1 ns
  Default gate width: 7 ns
  Notes: Two APD detectors; timing resolution optimized for time-gated CW-STED.

• Generic Monochrome CCD camera
  Charge-Coupled Device
  Type: Charge-Coupled Device
  Pixel pitch: 4.65 µm
  Sensor format: 1280 x 960
  Notes: 1/2-inch format camera.

Objectives

View this microscope's objectives in the catalogue

• LMPlanFL N 20x/0.40 AIR
  Olympus
  Model: LMPlanFL N 20x/0.40
  Magnification / NA: 20x/0.4
  Immersion: Air
  Correction: Plan Fluorite
  Working distance: 12.0 mm
  Product code: 1-U2N345
  Installed: Yes

• UPlanSApo 60x/1.2 W WATER
  Olympus
  Model: UPlanSApo 60x/1.2 W
  Magnification / NA: 60x/1.2
  Immersion: Water
  Correction: Plan Apochromat
  Working distance: 0.28 mm
  Product code: N6432600
  Installed: Yes

• UPlanSApo 100x/1.40 Oil OIL
  Olympus
  Model: UPlanSApo 100x/1.40 Oil
  Magnification / NA: 100x/1.4
  Immersion: Oil
  Correction: Plan Apochromat
  Working distance: 0.13 mm
  Product code: 1-U2B836
  Installed: Yes

Optical modulators

• Spatial Light Modulator
  Abberior
  Type: Spatial Light Modulator
  Supported phase masks: Vortex, Bottle, 3D-STED
  Notes: Used for Easy3D STED phase-pattern generation and adaptive optics workflows.

Illumination control

• RESCue STED
  Adaptive illumination
  Method: RESCue STED
  Default enabled: No
  Notes: Real-time adaptive STED decision logic used to reduce photobleaching and light dose during live-cell super-resolution imaging.

Optical-path elements

• Emission Filter Wheel
  Emission
  Element type: Filter Wheel
  Pos 1: Bandpass (FF01-525/25) — emission 525/25 nm
  Pos 2: Bandpass — emission 615/10 nm
  Pos 3: Bandpass (ET685/35) — emission 685/35 nm

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Latest QC measurements

Laser performance

No recent laser-performance measurements are recorded.

Optical resolution (PSF and alignment)

No recent PSF or chromatic-shift measurements are recorded for these objectives.

Objectives without recent measurements

• LMPlanFL N 20x/0.40 AIR (20x_air)

• UPlanSApo 60x/1.2 W WATER (60x_water)

• UPlanSApo 100x/1.40 Oil OIL (100x_oil)

Information still to confirm

Some details in the facility record are incomplete. If any of these matter for your experiment, please check them with AIC Turku staff.

• Details needed for this configuration:

• Software version (software[].version)

• Detector peak quantum efficiency (%) (hardware.detectors[].qe_peak_pct)