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Abberior STED

Work in progress

This dashboard is a work in progress. Please independently verify critical information, as not all features are working yet.

Current Status: 🟢 Online

Operational

📊 View QC & Maintenance History 🔬 Open Virtual Microscope

Abberior STED
Manufacturer
Abberior
Model
STED/RESOLFT
Stand
Inverted
Eyepieces
Trinocular
Location
BioCity, 5th floor, BioPhysics room 5086
Purchased
—
Funding
—
Modalities
Stimulated Emission Depletion RESOLFT Point-Scanning Confocal
Modules
Easy3D STED Adaptive Optics RESCue STED


Software Environment

  • Abberior Imspector
    Acquisition
    Role: Acquisition

Hardware Configuration

💡 Light Sources

  • 485 nm Laser PicoQuant LDH-D-C-485
    PicoQuant
    Type: Laser
    Role: Excitation
    Timing mode: Pulsed
    Pulse width: 80 ps
    Repetition rate: 40 MHz
    Wavelength: 485 nm
    Notes: Pulsed excitation source for the 488 nm channel.

  • 561 nm Laser PicoQuant PDL-T 561
    PicoQuant
    Type: Laser
    Role: Excitation
    Timing mode: Pulsed
    Pulse width: 80 ps
    Repetition rate: 40 MHz
    Wavelength: 561 nm
    Notes: Pulsed excitation source for the 561 nm channel.

  • 640 nm Laser PicoQuant LDH-D-C-640
    PicoQuant
    Type: Laser
    Role: Excitation
    Timing mode: Pulsed
    Pulse width: 80 ps
    Repetition rate: 40 MHz
    Wavelength: 640 nm
    Notes: Pulsed excitation source for the 640 nm channel.

  • 580 nm Laser MPB Communications VFL-P-1000-580
    MPB Communications
    Type: Laser
    Role: Depletion
    Timing mode: Continuous Wave
    Depletion targets: 488 nm
    Wavelength: 580 nm
    Notes: Continuous-wave STED depletion source for the 488 nm excitation channel.

  • 775 nm Laser OneFive / NKT Photonics Katana HP 775
    OneFive / NKT Photonics
    Type: Laser
    Role: Depletion
    Timing mode: Pulsed
    Pulse width: 600 ps
    Repetition rate: 40 MHz
    Depletion targets: 561 nm, 640 nm
    Wavelength: 775 nm
    Notes: Pulsed STED depletion source for the 561 nm and 640 nm excitation channels.

🧭 Scanner

  • Galvanometric Scanner
    Type: Galvanometric Scanner
    Notes: STED point scanning configuration.

📷 Detectors

  • Hamamatsu Photonics Photomultiplier Tube
    Photomultiplier Tube
    Type: Photomultiplier Tube
    Notes: General confocal detection channel.

  • Excelitas Technologies SPCM-AQRH-14-TR
    Avalanche Photodiode
    Type: Avalanche Photodiode
    Supports time gating: Yes
    Default gating delay: 1 ns
    Default gate width: 7 ns
    Notes: Two APD detectors; timing resolution optimized for time-gated CW-STED.

  • Generic Monochrome CCD camera
    Charge-Coupled Device
    Type: Charge-Coupled Device
    Pixel pitch: 4.65 µm
    Sensor format: 1280 x 960
    Notes: 1/2-inch format camera.

🔬 Objectives

  • LMPlanFL N 20x/0.40 20x/0.4 AIR
    Olympus
    Model: LMPlanFL N 20x/0.40
    Magnification / NA: 20x/0.4
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 12.0 mm
    Product code: 1-U2N345
    Installed: Yes

  • UPlanSApo 60x/1.2 W 60x/1.2 WATER
    Olympus
    Model: UPlanSApo 60x/1.2 W
    Magnification / NA: 60x/1.2
    Immersion: Water
    Correction: Plan Apochromat
    Working distance: 0.28 mm
    Product code: N6432600
    Installed: Yes

  • UPlanSApo 100x/1.40 Oil 100x/1.4 OIL
    Olympus
    Model: UPlanSApo 100x/1.40 Oil
    Magnification / NA: 100x/1.4
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.13 mm
    Product code: 1-U2B836
    Installed: Yes

🌀 Optical Modulators

  • Spatial Light Modulator
    Abberior
    Type: Spatial Light Modulator
    Supported phase masks: Vortex, Bottle, 3D-STED
    Notes: Used for Easy3D STED phase-pattern generation and adaptive optics workflows.

🧠 Adaptive Illumination Logic

  • RESCue STED
    Adaptive illumination
    Method: RESCue STED
    Default enabled: No
    Notes: Real-time adaptive STED decision logic used to reduce photobleaching and light dose during live-cell super-resolution imaging.

🎛️ Optical Path Elements

  • Emission Filter Wheel
    Emission
    Element type: Filter Wheel
    Pos 1: Bandpass (FF01-525/25) — emission 525/25 nm
    Pos 2: Bandpass — emission 615/10 nm
    Pos 3: Bandpass (ET685/35) — emission 685/35 nm

QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data

  • LMPlanFL N 20x/0.40 20x/0.4 AIR (20x_air)

  • UPlanSApo 60x/1.2 W 60x/1.2 WATER (60x_water)

  • UPlanSApo 100x/1.40 Oil 100x/1.4 OIL (100x_oil)

Metadata Completeness

  • Missing conditional fields (currently applicable):

  • software[].version — Software version

  • hardware.detectors[].qe_peak_pct — Detector peak quantum efficiency (%)

  • Legacy aliases in use (fallback accepted):

  • modules[].name → modules[].type

  • hardware.detectors[].pixel_size_um → hardware.detectors[].pixel_pitch_um

  • hardware.detectors[].min_nm → hardware.detectors[].collection_min_nm

  • hardware.detectors[].max_nm → hardware.detectors[].collection_max_nm

  • hardware.detectors[].channel_center_nm → hardware.detectors[].collection_center_nm

  • hardware.detectors[].bandwidth_nm → hardware.detectors[].collection_width_nm