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EVOS fl

Work in progress

This dashboard is a work in progress. Please independently verify critical information, as not all features are working yet.

Current Status: 🟢 Online

Operational

📊 View QC & Maintenance History 🔬 Open Virtual Microscope

EVOS fl
Manufacturer
Thermo Fisher / AMG
Model
FL
Stand
Inverted
Eyepieces
Camera-only
Location
Local Cell Culture Lab, Biocity 5th Floor
Purchased
2011
Funding
Turun Yliopisto / Biotekniikan keskus
Modalities
Widefield Fluorescence Transmitted Brightfield Phase Contrast
Modules
Universal Vessel Holder


Software Environment

Hardware Configuration

💡 Light Sources

  • LED Thermo Fisher / AMG Transmitted Light LED
    Thermo Fisher / AMG
    Type: LED
    Notes: Broad spectrum overhead LED used for brightfield and phase contrast.

  • 357 nm LED Thermo Fisher / AMG EVOS DAPI Light Cube LED
    Thermo Fisher / AMG
    Type: LED
    Wavelength: 357 nm
    Notes: Integrated LED inside the DAPI Light Cube

  • 445 nm LED Thermo Fisher / AMG EVOS CFP Light Cube LED
    Thermo Fisher / AMG
    Type: LED
    Wavelength: 445 nm
    Notes: Integrated LED inside the CFP Light Cube

  • 470 nm LED Thermo Fisher / AMG EVOS GFP Light Cube LED
    Thermo Fisher / AMG
    Type: LED
    Wavelength: 470 nm
    Notes: Integrated LED inside the GFP Light Cube

  • 531 nm LED Thermo Fisher / AMG EVOS RFP Light Cube LED
    Thermo Fisher / AMG
    Type: LED
    Wavelength: 531 nm
    Notes: Integrated LED inside the RFP Light Cube

  • 628 nm LED Thermo Fisher / AMG EVOS Cy5 Light Cube LED
    Thermo Fisher / AMG
    Type: LED
    Wavelength: 628 nm
    Notes: Integrated LED inside the Cy5 Light Cube

📷 Detectors

  • AMG (Thermo Fisher) Sony ICX285AQ Color CCD
    Charge-Coupled Device
    Type: Charge-Coupled Device
    Pixel pitch: 6.45 µm
    Notes: Integrated color camera for brightfield (1.4 Megapixels).

  • AMG (Thermo Fisher) AMF-4302 (Sony ICX285AL Monochrome CCD)
    Charge-Coupled Device
    Type: Charge-Coupled Device
    Pixel pitch: 6.45 µm
    Notes: Integrated monochrome camera for fluorescence (1.4 Megapixels).

🔬 Objectives

  • U Plan FL N 4x/0.13 PhP 4x/0.13 AIR
    Olympus
    Model: U Plan FL N 4x/0.13 PhP
    Magnification / NA: 4x/0.13
    Immersion: Air
    Correction: Fluorite
    Working distance: 16.4 mm
    AFC compatible: No
    Installed: Yes
    Specialties: phase
    Notes: PhP

  • Plan Fluor 10x/0.3 10x/0.3 AIR
    AMG (Thermo Fisher)
    Model: Plan Fluor 10x/0.3
    Magnification / NA: 10x/0.3
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 8.3 mm
    Product code: AMG-AMEP 4623
    AFC compatible: No
    Installed: Yes
    Notes: Fluorite (FL)

  • Plan Fluor 20x/0.45 20x/0.45 AIR
    AMG (Thermo Fisher)
    Model: Plan Fluor 20x/0.45
    Magnification / NA: 20x/0.45
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 7.1 mm
    Product code: AMG-AMEP 4624
    AFC compatible: No
    Installed: Yes
    Notes: Fluorite (FL)

  • Plan Fluor 40x/0.65 40x/0.65 AIR
    AMG (Thermo Fisher)
    Model: Plan Fluor 40x/0.65
    Magnification / NA: 40x/0.65
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 2.8 mm
    Product code: AMG-AMEP 4625
    AFC compatible: No
    Installed: Yes
    Notes: Fluorite (FL)

🎛️ Optical Path Elements

  • Light Cube Turret
    Cube
    Element type: Turret
    Details: Filter sets must be changed manually. Only AIC personnel are allowed to change filter cubes.
    Pos 1: DAPI (ZP-EPI-9001) — emission 447/60 nm — Included with base unit. Excitation: 357/44 nm. Dichroic Cutoff: 400 nm.
    Pos 2: CFP (AMEP-4653) — emission 510/42 nm — Excitation: 445/45 nm. Dichroic Cutoff: 480 nm.
    Pos 3: GFP/Alexa 488 (ZP-EPI-9002) — emission 510/42 nm — Included with base unit. Excitation: 470/22 nm. Dichroic Cutoff: 495 nm.
    Pos 4: RFP/Alexa 555 (ZP-EPI-9003) — emission 593/40 nm — Included with base unit. Excitation: 531/40 nm. Dichroic Cutoff: 560 nm.
    Pos 5: Cy5/Alexa 647 (AMEP-4656) — emission 692/40 nm — Excitation: 628/40 nm. Dichroic Cutoff: 660 nm.

QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data

  • U Plan FL N 4x/0.13 PhP 4x/0.13 AIR (4x_air)

  • Plan Fluor 10x/0.3 10x/0.3 AIR (10x_air)

  • Plan Fluor 20x/0.45 20x/0.45 AIR (20x_air)

  • Plan Fluor 40x/0.65 40x/0.65 AIR (40x_air)

Metadata Completeness

  • Missing conditional fields (currently applicable):

  • software[].version — Software version

  • Legacy aliases in use (fallback accepted):

  • modules[].name → modules[].type

  • hardware.detectors[].pixel_size_um → hardware.detectors[].pixel_pitch_um

  • hardware.detectors[].min_nm → hardware.detectors[].collection_min_nm

  • hardware.detectors[].max_nm → hardware.detectors[].collection_max_nm

  • hardware.detectors[].channel_center_nm → hardware.detectors[].collection_center_nm

  • hardware.detectors[].bandwidth_nm → hardware.detectors[].collection_width_nm