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EVOS fl

About this microscope

Here you’ll find the hardware and capabilities currently recorded for this microscope. If you are planning an experiment, AIC Turku staff can help with access, training, and configuration choices.

Current status: 🟢 Online

Operational

QC and maintenance history Open Virtual Microscope

EVOS fl microscope
Manufacturer
Thermo Fisher / AMG
Model
FL
Stand
Inverted
Eyepieces
Camera-only
Location
Local Cell Culture Lab, Biocity 5th Floor
Purchased
2011
Funding
Turun Yliopisto / Biotekniikan keskus
Capabilities
These are the capabilities recorded for this microscope. Open the optical-route details below to see how the hardware is connected.
Widefield fluorescenceTransmitted brightfieldPhase contrast
Methods and the light path that records them
Each method below is explicitly associated in the instrument record with the physical light path that implements it. An imaging mode or contrast method that appears above but not here has no recorded method-to-path mapping yet.
  • Widefield fluorescence: Widefield fluorescence
  • Transmitted light: Transmitted brightfield, Phase contrast
Modules
Universal Vessel Holder


Software

  • On-board EVOS interface
    Acquisition
    Role: Acquisition
    Version: Not documented
    Notes: no external software, USB storage

Hardware

Light sources

  • LED Thermo Fisher / AMG Transmitted Light LED
    Thermo Fisher / AMG
    Type: LED
    Notes: Broad spectrum overhead LED used for brightfield and phase contrast.

  • 357 nm LED Thermo Fisher / AMG EVOS DAPI Light Cube LED
    Thermo Fisher / AMG
    Type: LED
    Wavelength: 357 nm
    Notes: Integrated LED inside the DAPI Light Cube

  • 445 nm LED Thermo Fisher / AMG EVOS CFP Light Cube LED
    Thermo Fisher / AMG
    Type: LED
    Wavelength: 445 nm
    Notes: Integrated LED inside the CFP Light Cube

  • 470 nm LED Thermo Fisher / AMG EVOS GFP Light Cube LED
    Thermo Fisher / AMG
    Type: LED
    Wavelength: 470 nm
    Notes: Integrated LED inside the GFP Light Cube

  • 531 nm LED Thermo Fisher / AMG EVOS RFP Light Cube LED
    Thermo Fisher / AMG
    Type: LED
    Wavelength: 531 nm
    Notes: Integrated LED inside the RFP Light Cube

  • 628 nm LED Thermo Fisher / AMG EVOS Cy5 Light Cube LED
    Thermo Fisher / AMG
    Type: LED
    Wavelength: 628 nm
    Notes: Integrated LED inside the Cy5 Light Cube

Detectors

  • AMG (Thermo Fisher) Sony ICX285AQ Color CCD
    Charge-Coupled Device
    Type: Charge-Coupled Device
    Pixel pitch: 6.45 µm
    Notes: Integrated color camera for brightfield (1.4 Megapixels).

  • AMG (Thermo Fisher) AMF-4302 (Sony ICX285AL Monochrome CCD)
    Charge-Coupled Device
    Type: Charge-Coupled Device
    Pixel pitch: 6.45 µm
    Notes: Integrated monochrome camera for fluorescence (1.4 Megapixels).

Objectives

View this microscope's objectives in the catalogue

  • U Plan FL N 4x/0.13 PhP AIR
    Olympus
    Model: U Plan FL N 4x/0.13 PhP
    Magnification / NA: 4x/0.13
    Immersion: Air
    Correction: Fluorite
    Working distance: 16.4 mm
    AFC compatible: No
    Installed: Yes
    Specialties: phase
    Notes: PhP

  • Plan Fluor 10x/0.3 AIR
    AMG (Thermo Fisher)
    Model: Plan Fluor 10x/0.3
    Magnification / NA: 10x/0.3
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 8.3 mm
    Product code: AMG-AMEP 4623
    AFC compatible: No
    Installed: Yes
    Notes: Fluorite (FL)

  • Plan Fluor 20x/0.45 AIR
    AMG (Thermo Fisher)
    Model: Plan Fluor 20x/0.45
    Magnification / NA: 20x/0.45
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 7.1 mm
    Product code: AMG-AMEP 4624
    AFC compatible: No
    Installed: Yes
    Notes: Fluorite (FL)

  • Plan Fluor 40x/0.65 AIR
    AMG (Thermo Fisher)
    Model: Plan Fluor 40x/0.65
    Magnification / NA: 40x/0.65
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 2.8 mm
    Product code: AMG-AMEP 4625
    AFC compatible: No
    Installed: Yes
    Notes: Fluorite (FL)

Optical-path elements

  • Light Cube Turret
    Cube
    Element type: Turret
    Details: Filter sets must be changed manually. Only AIC personnel are allowed to change filter cubes.
    Pos 1: DAPI (ZP-EPI-9001) — emission 447/60 nm — Included with base unit. Excitation: 357/44 nm. Dichroic Cutoff: 400 nm.
    Pos 2: CFP (AMEP-4653) — emission 510/42 nm — Excitation: 445/45 nm. Dichroic Cutoff: 480 nm.
    Pos 3: GFP/Alexa 488 (ZP-EPI-9002) — emission 510/42 nm — Included with base unit. Excitation: 470/22 nm. Dichroic Cutoff: 495 nm.
    Pos 4: RFP/Alexa 555 (ZP-EPI-9003) — emission 593/40 nm — Included with base unit. Excitation: 531/40 nm. Dichroic Cutoff: 560 nm.
    Pos 5: Cy5/Alexa 647 (AMEP-4656) — emission 692/40 nm — Excitation: 628/40 nm. Dichroic Cutoff: 660 nm.

Latest QC measurements

Laser performance

No recent laser-performance measurements are recorded.

Optical resolution (PSF and alignment)

No recent PSF or chromatic-shift measurements are recorded for these objectives.

Objectives without recent measurements

  • U Plan FL N 4x/0.13 PhP AIR (4x_air)

  • Plan Fluor 10x/0.3 AIR (10x_air)

  • Plan Fluor 20x/0.45 AIR (20x_air)

  • Plan Fluor 40x/0.65 AIR (40x_air)

Information still to confirm

Some details in the facility record are incomplete. If any of these matter for your experiment, please check them with AIC Turku staff.

  • Details needed for this configuration:

  • Software version (software[].version)