Zeiss LSM 880 with AiryScan
Work in progress
This dashboard is a work in progress. Please independently verify critical information, as not all features are working yet.
Current Status: 🟡 Warning
Limited service: images remain usable, especially with unidirectional scanning, but the scan-field test failed and some bidirectional scan speeds show the issue. A VM800 startup communication error also remains open. The 40x multi-immersion objective's correction collar was repaired and the objective returned to normal operation.
Software Environment
- Zeiss ZEN 2.3 SP1 (black edition) (vFP3 HF 30 FP3 (build 14.0.30.201))
Acquisition
Role: Acquisition
Version:FP3 HF 30 FP3 (build 14.0.30.201)
Notes: Version recorded during vendor preventive maintenance on 2026-05-13.
Hardware Configuration
🌡️ Environmental Control
- Environmental Control
controlled temperature
Temperature control: Yes
Notes: Environmental chamber with temperature control and heated stage inserts
💡 Light Sources
-
405 nm Laser Unknown 405 nm (Diode)
Unknown
Type: Laser
Wavelength:405 nm -
458 nm Laser Unknown 458 nm (Argon)
Unknown
Type: Laser
Wavelength:458 nm -
488 nm Laser Unknown 488 nm (Argon)
Unknown
Type: Laser
Wavelength:488 nm -
514 nm Laser Unknown 514 nm (Argon)
Unknown
Type: Laser
Wavelength:514 nm -
543 nm Laser Unknown 543 nm (HeNe)
Unknown
Type: Laser
Wavelength:543 nm -
633 nm Laser Unknown 633 nm (HeNe)
Unknown
Type: Laser
Wavelength:633 nm -
Halogen Lamp Zeiss Transmitted light illumination (HAL 100 / LED)
Zeiss
Type: Halogen Lamp
Role: Transmitted Illumination
Notes: Transmitted brightfield illumination source for the inverted stand.
🧭 Scanner
- Resonant Scanner
Type: Resonant Scanner
Line rate:8000 Hz
📷 Detectors
-
Unknown PMT (Ch1)
Photomultiplier Tube
Type: Photomultiplier Tube
QE peak:30% -
Unknown Cooled PMT (Ch2)
Photomultiplier Tube
Type: Photomultiplier Tube
QE peak:30% -
Unknown Transmitted light PMT
Photomultiplier Tube
Type: Photomultiplier Tube
QE peak:30% -
Unknown 32-channel GaAsP spectral array detector
Photomultiplier Tube
Type: Photomultiplier Tube
QE peak:45% -
Unknown 32-channel AiryScan detector (GaAsP)
Photomultiplier Tube
Type: Photomultiplier Tube
QE peak:45%
🔬 Objectives
-
Plan-APOCHROMAT 10x/0.3 10x/0.3 AIR
Zeiss
Model: Plan-APOCHROMAT 10x/0.3
Magnification / NA:10x/0.3
Immersion: Air
Correction: Plan Apochromat
Working distance:2.0 mm
Product code:420640-9800
AFC compatible: No
Installed: Yes -
Plan-APOCHROMAT 20x/0.8 20x/0.8 AIR
Zeiss
Model: Plan-APOCHROMAT 20x/0.8
Magnification / NA:20x/0.8
Immersion: Air
Correction: Plan Apochromat
Working distance:0.55 mm
Product code:420650-9901
AFC compatible: No
Installed: Yes -
LD LCI Plan APOCHROMAT 40x/1.2 Imm Korr DIC (UV)VIS-IR 40x/1.2 MULTI-IMMERSION
Zeiss
Model: LD LCI Plan APOCHROMAT 40x/1.2 Imm Korr DIC (UV)VIS-IR
Magnification / NA:40x/1.2
Immersion: Multi-Immersion
Correction: Plan Apochromat
Working distance:0.41 mm
Product code:420862-9970
AFC compatible: No
Installed: Yes
Specialties: dic, ir_transmission
Notes: Imm Korr (multi-immersion, correction collar). Correction collar repaired during the 2026-05-13 to 2026-05-14 vendor preventive-maintenance visit (maintenance_id: maint_scope-zeiss-lsm-880-with-airyscan_20260513_preventive_maintenance); objective operational. -
C-Plan-APOCHROMAT 63x/1.4 Oil DIC UV-VIS-IR 63x/1.4 OIL
Zeiss
Model: C-Plan-APOCHROMAT 63x/1.4 Oil DIC UV-VIS-IR
Magnification / NA:63x/1.4
Immersion: Oil
Correction: Plan Apochromat
Working distance:0.14 mm
Product code:421782-9900
AFC compatible: No
Installed: Yes
Specialties: dic, ir_transmission
- LD LCI Plan-APOCHROMAT 25x/0.8 Imm Korr DIC (UV)VIS-IR 25x/0.8 MULTI-IMMERSIONAsk Staff to Install
Zeiss
Model: LD LCI Plan-APOCHROMAT 25x/0.8 Imm Korr DIC (UV)VIS-IR
Magnification / NA:25x/0.8
Immersion: Multi-Immersion
Correction: Plan Apochromat
Working distance:0.57 mm at cover glass 0.17
Product code:420852-9871
AFC compatible: No
Installed: No
Specialties: dic, ir_transmission
Notes: Optional objective. Imm Korr (Multi-immersion, correction collar)
- C-APOCHROMAT 63x/1.2 W Korr UV-VIS-IR 63x/1.2 WATERAsk Staff to Install
Zeiss
Model: C-APOCHROMAT 63x/1.2 W Korr UV-VIS-IR
Magnification / NA:63x/1.2
Immersion: Water
Correction: Apochromat
Working distance:0.28 mm at cover glass 0.17
Product code:421787-9970
AFC compatible: No
Installed: No
Specialties: ir_transmission
Notes: Optional objective. W Korr (Water immersion, correction collar)
🧭 Stages & Focus Drives
- Z Piezo Stage — Zeiss Piezo Z-stage
Zeiss
Type: Z Piezo Stage
⚙️ Advanced Hardware
- Infrared Reflection Autofocus
Installed: Yes
Type: Infrared Reflection Autofocus
🎛️ Optical Path Elements
- AiryScan Emission Wheel
Emission
Element type: Filter Wheel
Pos 1: AiryScan BP 420-488 + BP 495-550 — emission 454/68 nm, 522.5/55 nm
Pos 2: AiryScan BP 465-505 + LP 525 — emission 485/40 nm
Pos 3: AiryScan BP 420-488 + LP 555 — emission 454/68 nm
Pos 4: AiryScan BP 495-550 + LP 570 — emission 522.5/55 nm
Pos 5: AiryScan BP 555-620 + LP 645 — emission 587.5/65 nm
Pos 6: AiryScan BP 570-620 + LP 645 — emission 595/50 nm
QC Metrics: Latest Measurements
🔦 Laser Performance
-
405 nm laser power at objective (100%)
3.83 mW
laser.at_obj.405.100pct.power_mw -
458 nm laser power at objective (100%)
0.19 mW
laser.at_obj.458.100pct.power_mw -
488 nm laser power at objective (100%)
1.33 mW
laser.at_obj.488.100pct.power_mw -
543 nm laser power at objective (100%)
0.27 mW
laser.at_obj.543.100pct.power_mw -
633 nm laser power at objective (100%)
1.09 mW
laser.at_obj.633.100pct.power_mw -
Laser · Fiber · Argon · Max Power mW
12.54 mW
laser.fiber.argon.max_power_mw -
Laser · Fiber · Argon · Max Tube Current A
8.27 A
laser.fiber.argon.max_tube_current_a -
Laser · Fiber · Argon · Optimal Power mW
11.42 mW
laser.fiber.argon.optimal_power_mw -
Laser · Fiber · Argon · Optimal Tube Current A
6 A
laser.fiber.argon.optimal_tube_current_a -
Laser · Fiber · 405 · Power mW
13.55 mW
laser.fiber.405.power_mw -
Laser · Fiber · 543 · Power mW
0.95 mW
laser.fiber.543.power_mw -
Laser · Fiber · 633 · Power mW
5.12 mW
laser.fiber.633.power_mw -
Laser · Argon · Total Operating Hours
17550 h
laser.argon.total_operating_hours -
Laser · Argon · Hours Since Exchange
1903 h
laser.argon.hours_since_exchange
🔬 Optical Resolution (PSF & Alignment)
No recent PSF or chromatic shift metrics available for any objectives.
Objectives without recent data
-
Plan-APOCHROMAT 10x/0.3 10x/0.3 AIR (
10x_air) -
Plan-APOCHROMAT 20x/0.8 20x/0.8 AIR (
20x_air) -
LD LCI Plan APOCHROMAT 40x/1.2 Imm Korr DIC (UV)VIS-IR 40x/1.2 MULTI-IMMERSION (
40x_multi) -
C-Plan-APOCHROMAT 63x/1.4 Oil DIC UV-VIS-IR 63x/1.4 OIL (
63x_oil) -
LD LCI Plan-APOCHROMAT 25x/0.8 Imm Korr DIC (UV)VIS-IR 25x/0.8 MULTI-IMMERSION (
25x_multi) -
C-APOCHROMAT 63x/1.2 W Korr UV-VIS-IR 63x/1.2 WATER (
63x_water)
⚙️ Environment & Stage Performance
-
Stage · Tile Scan Error X px
2 px
stage.tile_scan_error_x_px -
Stage · Tile Scan Error Y px
0 px
stage.tile_scan_error_y_px -
Stage · Tile Scan Error Total px
4 px
stage.tile_scan_error_total_px -
Signal To Noise · Detector SNR Test Status
deferred
signal_to_noise.detector_snr_test_status -
Signal To Noise · Channel 1 Test Status
pass
signal_to_noise.channel_1_test_status -
Signal To Noise · Channel 2 Test Status
pass
signal_to_noise.channel_2_test_status -
Signal To Noise · Channel 3 Test Status
pass
signal_to_noise.channel_3_test_status -
Signal To Noise · 32 Channel Array Test Status
pass
signal_to_noise.32_channel_array_test_status -
Signal To Noise · Airyscan Test Status
pass
signal_to_noise.airyscan_test_status