Zeiss LSM 880 with AiryScan

About this microscope

Here you’ll find the hardware and capabilities currently recorded for this microscope. If you are planning an experiment, AIC Turku staff can help with access, training, and configuration choices.

Current status: 🟡 Warning

Limited service: images remain usable, especially with unidirectional scanning, but the scan-field test failed and some bidirectional scan speeds show the issue. A VM800 startup communication error also remains open. The 40x multi-immersion objective's correction collar was repaired and the objective returned to normal operation.

QC and maintenance history Open Virtual Microscope

Manufacturer

Zeiss

Model

LSM 880 with AiryScan

Stand

Inverted

Eyepieces

Trinocular

Location

BioCity, 5th floor, room 5149

Purchased

—

Funding

—

Capabilities

These are the capabilities recorded for this microscope. Open the optical-route details below to see how the hardware is connected.

Confocal point scanningISM (AiryScan)Transmitted brightfieldDICSpectral ImagingLive cell imagingSuper-resolution

Methods and the light path that records them

Each method below is explicitly associated in the instrument record with the physical light path that implements it. An imaging mode or contrast method that appears above but not here has no recorded method-to-path mapping yet.

• Point-scanning confocal: Confocal point scanning, ISM (AiryScan)
• Transmitted light: Transmitted brightfield, DIC

Modules

AiryScan Detector/Module Incubation Hardware Autofocus Piezo Z-Stage High-Performance Workstation

Software

• Zeiss ZEN 2.3 SP1 (black edition) (vFP3 HF 30 FP3 (build 14.0.30.201))
  Acquisition
  Role: Acquisition
  Version: FP3 HF 30 FP3 (build 14.0.30.201)
  Notes: Version recorded during vendor preventive maintenance on 2026-05-13.

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Hardware

Environmental control

• Environmental Control
  controlled temperature
  Temperature control: Yes
  Notes: Environmental chamber with temperature control and heated stage inserts

Light sources

• 405 nm Laser Unknown 405 nm (Diode)
  Unknown
  Type: Laser
  Role: Excitation
  Wavelength: 405 nm

• 458 nm Laser Unknown 458 nm (Argon)
  Unknown
  Type: Laser
  Role: Excitation
  Wavelength: 458 nm

• 488 nm Laser Unknown 488 nm (Argon)
  Unknown
  Type: Laser
  Role: Excitation
  Wavelength: 488 nm

• 514 nm Laser Unknown 514 nm (Argon)
  Unknown
  Type: Laser
  Role: Excitation
  Wavelength: 514 nm

• 543 nm Laser Unknown 543 nm (HeNe)
  Unknown
  Type: Laser
  Role: Excitation
  Wavelength: 543 nm

• 633 nm Laser Unknown 633 nm (HeNe)
  Unknown
  Type: Laser
  Role: Excitation
  Wavelength: 633 nm

• Halogen Lamp Zeiss Transmitted light illumination (HAL 100 / LED)
  Zeiss
  Type: Halogen Lamp
  Role: Transmitted Illumination
  Notes: Transmitted brightfield illumination source for the inverted stand.

Scanner

• Galvanometric Scanner
  Zeiss LSM 880 galvanometric scan unit
  Type: Galvanometric Scanner
  Manufacturer: Zeiss
  Model: LSM 880 galvanometric scan unit

Detectors

• Zeiss Side PMT Ch1
  Photomultiplier Tube
  Type: Photomultiplier Tube
  QE peak: 30%

• Zeiss Temperature-controlled side PMT Ch2
  Photomultiplier Tube
  Type: Photomultiplier Tube
  QE peak: 30%

• Zeiss T-PMT transmitted-light detector
  Photomultiplier Tube
  Type: Photomultiplier Tube
  QE peak: 30%

• Zeiss Quasar 32-channel GaAsP spectral detector
  GaAsP Photomultiplier Tube
  Type: GaAsP Photomultiplier Tube
  QE peak: 45%

• Zeiss Airyscan first-generation 32-element GaAsP detector
  GaAsP Photomultiplier Tube
  Type: GaAsP Photomultiplier Tube
  QE peak: 45%

Objectives

View this microscope's objectives in the catalogue

• Plan-APOCHROMAT 10x/0.3 AIR
  Zeiss
  Model: Plan-APOCHROMAT 10x/0.3
  Magnification / NA: 10x/0.3
  Immersion: Air
  Correction: Plan Apochromat
  Working distance: 2.0 mm
  Product code: 420640-9800
  AFC compatible: No
  Installed: Yes

• Plan-APOCHROMAT 20x/0.8 AIR
  Zeiss
  Model: Plan-APOCHROMAT 20x/0.8
  Magnification / NA: 20x/0.8
  Immersion: Air
  Correction: Plan Apochromat
  Working distance: 0.55 mm
  Product code: 420650-9901
  AFC compatible: No
  Installed: Yes

• LD LCI Plan APOCHROMAT 40x/1.2 Imm Korr DIC (UV)VIS-IR MULTI-IMMERSION
  Zeiss
  Model: LD LCI Plan APOCHROMAT 40x/1.2 Imm Korr DIC (UV)VIS-IR
  Magnification / NA: 40x/1.2
  Immersion: Multi-Immersion
  Correction: Plan Apochromat
  Working distance: 0.41 mm
  Product code: 420862-9970
  AFC compatible: No
  Installed: Yes
  Specialties: dic, ir_transmission
  Notes: Imm Korr (multi-immersion, correction collar). Correction collar repaired during the 2026-05-13 to 2026-05-14 vendor preventive-maintenance visit (maintenance_id: maint_scope-zeiss-lsm-880-with-airyscan_20260513_preventive_maintenance); objective operational.

• C-Plan-APOCHROMAT 63x/1.4 Oil DIC UV-VIS-IR OIL
  Zeiss
  Model: C-Plan-APOCHROMAT 63x/1.4 Oil DIC UV-VIS-IR
  Magnification / NA: 63x/1.4
  Immersion: Oil
  Correction: Plan Apochromat
  Working distance: 0.14 mm
  Product code: 421782-9900
  AFC compatible: No
  Installed: Yes
  Specialties: dic, ir_transmission

• LD LCI Plan-APOCHROMAT 25x/0.8 Imm Korr DIC (UV)VIS-IR MULTI-IMMERSIONNot installed
  Zeiss
  Model: LD LCI Plan-APOCHROMAT 25x/0.8 Imm Korr DIC (UV)VIS-IR
  Magnification / NA: 25x/0.8
  Immersion: Multi-Immersion
  Correction: Plan Apochromat
  Working distance: 0.57 mm at cover glass 0.17
  Product code: 420852-9871
  AFC compatible: No
  Installed: No
  Specialties: dic, ir_transmission
  Notes: Optional objective. Imm Korr (Multi-immersion, correction collar)

• C-APOCHROMAT 63x/1.2 W Korr UV-VIS-IR WATERNot installed
  Zeiss
  Model: C-APOCHROMAT 63x/1.2 W Korr UV-VIS-IR
  Magnification / NA: 63x/1.2
  Immersion: Water
  Correction: Apochromat
  Working distance: 0.28 mm at cover glass 0.17
  Product code: 421787-9970
  AFC compatible: No
  Installed: No
  Specialties: ir_transmission
  Notes: Optional objective. W Korr (Water immersion, correction collar)

Stages and focus drives

• Z Piezo Stage — Zeiss Piezo Z-stage
  Zeiss
  Type: Z Piezo Stage

Autofocus and triggering

• Infrared Reflection Autofocus
  Installed: Yes
  Type: Infrared Reflection Autofocus

Optical-path elements

• AiryScan Emission Wheel
  Emission
  Element type: Filter Wheel
  Pos 1: Airyscan BP 420-480 + BP 495-550 — emission 450/60 nm, 522.5/55 nm
  Pos 2: Airyscan BP 420-480 + BP 495-620 — emission 450/60 nm, 557.5/125 nm
  Pos 3: Airyscan BP 420-480 + LP 605 — emission 450/60 nm
  Pos 4: Airyscan BP 465-505 + LP 525 — emission 485/40 nm
  Pos 5: Airyscan BP 495-550 + LP 570 — emission 522.5/55 nm
  Pos 6: Airyscan BP 570-620 + LP 645 — emission 595/50 nm

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Latest QC measurements

Laser performance

• 405 nm laser power at objective (100%)

  ---

  3.83 mW
  laser.at_obj.405.100pct.power_mw

• 458 nm laser power at objective (100%)

  ---

  0.19 mW
  laser.at_obj.458.100pct.power_mw

• 488 nm laser power at objective (100%)

  ---

  1.33 mW
  laser.at_obj.488.100pct.power_mw

• 543 nm laser power at objective (100%)

  ---

  0.27 mW
  laser.at_obj.543.100pct.power_mw

• 633 nm laser power at objective (100%)

  ---

  1.09 mW
  laser.at_obj.633.100pct.power_mw

• Laser · Fiber · Argon · Max Power mW

  ---

  12.54 mW
  laser.fiber.argon.max_power_mw

• Laser · Fiber · Argon · Max Tube Current A

  ---

  8.27 A
  laser.fiber.argon.max_tube_current_a

• Laser · Fiber · Argon · Optimal Power mW

  ---

  11.42 mW
  laser.fiber.argon.optimal_power_mw

• Laser · Fiber · Argon · Optimal Tube Current A

  ---

  6 A
  laser.fiber.argon.optimal_tube_current_a

• Laser · Fiber · 405 · Power mW

  ---

  13.55 mW
  laser.fiber.405.power_mw

• Laser · Fiber · 543 · Power mW

  ---

  0.95 mW
  laser.fiber.543.power_mw

• Laser · Fiber · 633 · Power mW

  ---

  5.12 mW
  laser.fiber.633.power_mw

• Laser · Argon · Total Operating Hours

  ---

  17550 h
  laser.argon.total_operating_hours

• Laser · Argon · Hours Since Exchange

  ---

  1903 h
  laser.argon.hours_since_exchange

Optical resolution (PSF and alignment)

No recent PSF or chromatic-shift measurements are recorded for these objectives.

Objectives without recent measurements

• Plan-APOCHROMAT 10x/0.3 AIR (10x_air)

• Plan-APOCHROMAT 20x/0.8 AIR (20x_air)

• LD LCI Plan APOCHROMAT 40x/1.2 Imm Korr DIC (UV)VIS-IR MULTI-IMMERSION (40x_multi)

• C-Plan-APOCHROMAT 63x/1.4 Oil DIC UV-VIS-IR OIL (63x_oil)

• LD LCI Plan-APOCHROMAT 25x/0.8 Imm Korr DIC (UV)VIS-IR MULTI-IMMERSION (25x_multi)

• C-APOCHROMAT 63x/1.2 W Korr UV-VIS-IR WATER (63x_water)

Environment and stage performance

• Stage · Tile Scan Error X px

  ---

  2 px
  stage.tile_scan_error_x_px

• Stage · Tile Scan Error Y px

  ---

  0 px
  stage.tile_scan_error_y_px

• Stage · Tile Scan Error Total px

  ---

  4 px
  stage.tile_scan_error_total_px

• Signal To Noise · Detector SNR Test Status

  ---

  deferred
  signal_to_noise.detector_snr_test_status

• Signal To Noise · Channel 1 Test Status

  ---

  pass
  signal_to_noise.channel_1_test_status

• Signal To Noise · Channel 2 Test Status

  ---

  pass
  signal_to_noise.channel_2_test_status

• Signal To Noise · Channel 3 Test Status

  ---

  pass
  signal_to_noise.channel_3_test_status

• Signal To Noise · 32 Channel Array Test Status

  ---

  pass
  signal_to_noise.32_channel_array_test_status

• Signal To Noise · Airyscan Test Status

  ---

  pass
  signal_to_noise.airyscan_test_status