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3i CSU-W1 Spinning Disk

Work in progress

This dashboard is a work in progress. Please independently verify critical information, as not all features are working yet.

Current Status: 🟡 Warning

Objective issue fixed, but laser startup delay, camera errors, and Y-montage bug remain

📊 View QC & Maintenance History 🔬 Open Virtual Microscope

3i CSU-W1 Spinning Disk
Manufacturer
3i / Zeiss
Model
Marianas CSU-W1 Spinning Disk Confocal
Stand
Inverted
Eyepieces
Trinocular
Location
BioCity, 5th floor, room 5149
Purchased
2015
Funding
—
Capabilities
Use these chips to quickly decide if this microscope supports your planned imaging mode, contrast method, or readout.
Confocal spinning diskWidefield fluorescenceTransmitted brightfieldPhase contrastDICLive cell imagingFRAPPhotoactivation
Modules
FRAP Module Piezo Z-Stage Hardware Autofocus Incubation


Software Environment

  • 3i SlideBook (v6)
    Acquisition
    Role: Acquisition
    Version: 6

Hardware Configuration

🌡️ Environmental Control

  • Environmental Control
    controlled temperature, controlled CO2
    Temperature control: Yes
    CO2 control: Yes
    O2 control: No
    Notes: Okolab Full Enclosure Incubator with temperature and digital CO2 control

💡 Light Sources

  • 405 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 405 nm
    Power: 100 mW

  • 488 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 488 nm
    Power: 150 mW

  • 561 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 561 nm
    Power: 100 mW

  • 640 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 640 nm
    Power: 100 mW

  • 730 nm Laser 3i SingleLine 730nm Laser Launch
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 730 nm
    Power: 30 mW
    Notes: Near-IR excitation line delivered via the CSU-W1 near-IR illumination port. Requires the installed IR/VIS combining dichroic, disk dichroic, and IR emitter to be documented explicitly in the light path.

  • LED Excelitas X-Cite XLED1
    Excelitas
    Type: LED
    Notes: 4-channel LED light source for DAPI, FITC, TRITC/Texas Red, and Cy5-class widefield fluorescence

🧭 Scanner

  • Spinning Disk Scanner
    Type: Spinning Disk Scanner
    Pinhole: 50 µm
    Notes: Yokogawa CSU-W1 with 50 µm pinhole disk. Appropriate for standard live-cell spinning-disk confocal imaging; less optimal than a 25 µm disk for low-magnification confocal sectioning.

📷 Detectors

  • Hamamatsu ORCA-Flash4.0
    Scientific CMOS
    Type: Scientific CMOS
    Pixel pitch: 6.5 µm
    Sensor format: 2048 x 2048
    QE peak: 82%

  • Photometrics Evolve
    Electron-Multiplying CCD
    Type: Electron-Multiplying CCD
    Pixel pitch: 16 µm
    Sensor format: 512 x 512
    QE peak: 95%
    Notes: 10 MHz back-illuminated EMCCD

🔬 Objectives

  • Plan-Apochromat 10x/0.45 Ph1 M27 10x/0.45 AIR
    Zeiss
    Model: Plan-Apochromat 10x/0.45 Ph1 M27
    Magnification / NA: 10x/0.45
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 2.0 mm
    Product code: 420641-9910
    AFC compatible: No
    Installed: Yes
    Specialties: phase

  • Plan-Apochromat 20x/0.8 20x/0.8 AIR
    Zeiss
    Model: Plan-Apochromat 20x/0.8
    Magnification / NA: 20x/0.8
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 0.55 mm
    Product code: 440640-9903-000
    AFC compatible: No
    Installed: Yes

  • LD C-APOCHROMAT 40x/1.1 W M27 40x/1.1 WATER
    Zeiss
    Model: LD C-APOCHROMAT 40x/1.1 W M27
    Magnification / NA: 40x/1.1
    Immersion: Water
    Correction: Apochromat
    Working distance: 0.62 mm
    Product code: 421867-9970
    AFC compatible: No
    Installed: Yes
    Specialties: coverslip_correction, ir_transmission

  • Plan-Apochromat 63x/1.4 Oil DIC M27 63x/1.4 OIL
    Zeiss
    Model: Plan-Apochromat 63x/1.4 Oil DIC M27
    Magnification / NA: 63x/1.4
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.19 mm
    Product code: 420782-9900
    AFC compatible: No
    Installed: Yes
    Specialties: dic

  • Plan-Apochromat 100x/1.4 Oil DIC M27 100x/1.4 OIL
    Zeiss
    Model: Plan-Apochromat 100x/1.4 Oil DIC M27
    Magnification / NA: 100x/1.4
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.17 mm
    Product code: 420792-9901
    AFC compatible: No
    Installed: Yes
    Specialties: dic

  • C-APOCHROMAT 63x/1.2 W Korr UV-VIS-IR 63x/1.2 WATERAsk Staff to Install
    Zeiss
    Model: C-APOCHROMAT 63x/1.2 W Korr UV-VIS-IR
    Magnification / NA: 63x/1.2
    Immersion: Water
    Correction: Apochromat
    Working distance: 0.28 mm at cover glass 0.17
    Product code: 421787-9970
    AFC compatible: No
    Installed: No
    Specialties: ir_transmission, correction_collar
    Notes: Optional objective. Water-immersion correction-collar objective.

🧭 Stages & Focus Drives

  • Z Piezo Stage — ASI PZ-2000
    ASI
    Type: Z Piezo Stage

⚙️ Advanced Hardware

  • Infrared Reflection Autofocus
    Installed: Yes
    Type: Infrared Reflection Autofocus

🎛️ Optical Path Elements

  • XLED Excitation Filters
    Excitation
    Element type: Filter Wheel
    Pos 1: Bandpass (FF01-387/11-25) — emission 387/11 nm
    Pos 2: Bandpass (FF02-485/20-25) — emission 485/20 nm
    Pos 3: Bandpass (FF01-560/25-25) — emission 560/25 nm
    Pos 4: Bandpass (FF01-650/13-25) — emission 650/13 nm

  • CSU-W1 Dichroic Slider
    Dichroic
    Element type: Slider
    Pos 1: Quad-band Dichroic (Di01-T405/488/568/647) — emission 440/25 nm, 521/25 nm, 607/25 nm, 700/25 nm — Standard multiband spinning-disk dichroic for 405/488/561/640-class excitation.
    Pos 2: NIR dichroic position — Placeholder for the installed NIR dichroic used with the 730 nm excitation / 809 nm emission route.

  • Widefield Dichroic
    Dichroic
    Element type: Fixed
    Pos 1: Multiband Dichroic (FF410/504/582/669-Di01-25x36) — emission 440/25 nm, 521/25 nm, 607/25 nm, 700/25 nm

  • CSU-W1 Emission Wheel
    Emission
    Element type: Filter Wheel
    Pos 1: Quad-band emitter (FF01-440/521/607/700-25) — emission 440/25 nm, 521/25 nm, 607/25 nm, 700/25 nm — Quad-band emitter for multicolor spinning-disk imaging
    Pos 2: DAPI (FF01-445/45-25) — emission 445/45 nm
    Pos 3: GFP — emission 525/50 nm — Previously 525/30 (FF01-525/30-25 in the old PDF) before 2021-08-18.
    Pos 4: Cy3 / Alexa 568 (FF01-617/73-25) — emission 617/73 nm
    Pos 5: Cy5 / Alexa 647 (FF01-692/40-25) — emission 692/40 nm
    Pos 6: Alexa 750 (FF02-809/81-25) — emission 809/81 nm

  • Widefield Emission
    Emission
    Element type: Fixed
    Pos 1: Multiband Bandpass (FF01-440/521/607/700-25) — emission 440/25 nm, 521/25 nm, 607/25 nm, 700/25 nm

QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data

  • Plan-Apochromat 10x/0.45 Ph1 M27 10x/0.45 AIR (10x_air)

  • Plan-Apochromat 20x/0.8 20x/0.8 AIR (20x_air)

  • LD C-APOCHROMAT 40x/1.1 W M27 40x/1.1 WATER (40x_water)

  • Plan-Apochromat 63x/1.4 Oil DIC M27 63x/1.4 OIL (63x_oil)

  • Plan-Apochromat 100x/1.4 Oil DIC M27 100x/1.4 OIL (100x_oil)

  • LD LCI Plan-APOCHROMAT 25x/0.8 Imm Korr DIC (UV)VIS-IR 25x/0.8 MULTI-IMMERSION (25x_multi)

  • C-APOCHROMAT 63x/1.2 W Korr UV-VIS-IR 63x/1.2 WATER (63x_water_c_apo)