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3i CSU-W1 Spinning Disk

Work in progress

This dashboard is a work in progress. Please independently verify critical information, as not all features are working yet.

Current Status: 🟡 Warning

Objective issue fixed, but laser startup delay, camera errors, and Y-montage bug remain

📊 View QC & Maintenance History 🔬 Open Virtual Microscope

3i CSU-W1 Spinning Disk
Manufacturer
3i / Zeiss
Model
Marianas CSU-W1 Spinning Disk Confocal
Stand
Inverted
Eyepieces
Trinocular
Location
BioCity, 5th floor, room 5149
Purchased
2015
Funding
—
Modalities
Spinning-Disk Confocal Widefield Fluorescence Fluorescence Recovery After Photobleaching Photoactivation Transmitted Brightfield
Modules
FRAP Module Piezo Z-Stage Hardware Autofocus Incubation


Software Environment

  • 3i SlideBook (v6)
    Acquisition
    Role: Acquisition
    Version: 6

Hardware Configuration

🌡️ Environmental Control

  • Environmental Control
    controlled temperature, controlled CO2
    Temperature control: Yes
    CO2 control: Yes
    O2 control: No
    Notes: Okolab Full Enclosure Incubator with temperature and digital CO2 control

💡 Light Sources

  • 405 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 405 nm
    Power: 100 mW

  • 488 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 488 nm
    Power: 150 mW

  • 561 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 561 nm
    Power: 100 mW

  • 640 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 640 nm
    Power: 100 mW

  • 730 nm Laser 3i SingleLine 730nm Laser Launch
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 730 nm
    Power: 30 mW
    Notes: Near-IR excitation line delivered via the CSU-W1 near-IR illumination port. Requires the installed IR/VIS combining dichroic, disk dichroic, and IR emitter to be documented explicitly in the light path.

  • LED Excelitas X-Cite XLED1
    Excelitas
    Type: LED
    Notes: 4-channel LED light source for DAPI, FITC, TRITC/Texas Red, and Cy5-class widefield fluorescence

🧭 Scanner

  • Spinning Disk Scanner
    Type: Spinning Disk Scanner
    Pinhole: 50 µm
    Notes: Yokogawa CSU-W1 with 50 µm pinhole disk. Appropriate for standard live-cell spinning-disk confocal imaging; less optimal than a 25 µm disk for low-magnification confocal sectioning.

📷 Detectors

  • Hamamatsu ORCA-Flash4.0
    Scientific CMOS
    Type: Scientific CMOS
    Pixel pitch: 6.5 µm
    Sensor format: 2048 x 2048
    QE peak: 82%

  • Photometrics Evolve
    Electron-Multiplying CCD
    Type: Electron-Multiplying CCD
    Pixel pitch: 16 µm
    Sensor format: 512 x 512
    QE peak: 95%
    Notes: 10 MHz back-illuminated EMCCD

🔬 Objectives

  • Plan-Apochromat 10x/0.45 Ph1 M27 10x/0.45 AIR
    Zeiss
    Model: Plan-Apochromat 10x/0.45 Ph1 M27
    Magnification / NA: 10x/0.45
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 2.0 mm
    Product code: 420641-9910
    AFC compatible: No
    Installed: Yes
    Specialties: phase

  • Plan-Apochromat 20x/0.8 20x/0.8 AIR
    Zeiss
    Model: Plan-Apochromat 20x/0.8
    Magnification / NA: 20x/0.8
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 0.55 mm
    Product code: 440640-9903-000
    AFC compatible: No
    Installed: Yes

  • LD C-APOCHROMAT 40x/1.1 W M27 40x/1.1 WATER
    Zeiss
    Model: LD C-APOCHROMAT 40x/1.1 W M27
    Magnification / NA: 40x/1.1
    Immersion: Water
    Correction: Apochromat
    Working distance: 0.62 mm
    Product code: 421867-9970
    AFC compatible: No
    Installed: Yes
    Specialties: coverslip_correction, ir_transmission

  • Plan-Apochromat 63x/1.4 Oil DIC M27 63x/1.4 OIL
    Zeiss
    Model: Plan-Apochromat 63x/1.4 Oil DIC M27
    Magnification / NA: 63x/1.4
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.19 mm
    Product code: 420782-9900
    AFC compatible: No
    Installed: Yes
    Specialties: dic

  • Plan-Apochromat 100x/1.4 Oil DIC M27 100x/1.4 OIL
    Zeiss
    Model: Plan-Apochromat 100x/1.4 Oil DIC M27
    Magnification / NA: 100x/1.4
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.17 mm
    Product code: 420792-9901
    AFC compatible: No
    Installed: Yes
    Specialties: dic

  • C-APOCHROMAT 63x/1.2 W Korr UV-VIS-IR 63x/1.2 WATERAsk Staff to Install
    Zeiss
    Model: C-APOCHROMAT 63x/1.2 W Korr UV-VIS-IR
    Magnification / NA: 63x/1.2
    Immersion: Water
    Correction: Apochromat
    Working distance: 0.28 mm at cover glass 0.17
    Product code: 421787-9970
    AFC compatible: No
    Installed: No
    Specialties: ir_transmission, correction_collar
    Notes: Optional objective. Water-immersion correction-collar objective.

🧭 Stages & Focus Drives

  • Z Piezo Stage — ASI PZ-2000
    ASI
    Type: Z Piezo Stage

⚙️ Advanced Hardware

  • Infrared Reflection Autofocus
    Installed: Yes
    Type: Infrared Reflection Autofocus

🎛️ Optical Path Elements

  • XLED Excitation Filters
    Excitation
    Element type: Filter Wheel
    Pos 1: Bandpass (FF01-387/11-25) — emission 387/11 nm
    Pos 2: Bandpass (FF02-485/20-25) — emission 485/20 nm
    Pos 3: Bandpass (FF01-560/25-25) — emission 560/25 nm
    Pos 4: Bandpass (FF01-650/13-25) — emission 650/13 nm

  • CSU-W1 Dichroic Slider
    Dichroic
    Element type: Slider
    Pos 1: Quad-band Dichroic (Di01-T405/488/568/647) — emission 440/25 nm, 521/25 nm, 607/25 nm, 700/25 nm — Standard multiband spinning-disk dichroic for 405/488/561/640-class excitation.
    Pos 2: NIR dichroic position — Placeholder for the installed NIR dichroic used with the 730 nm excitation / 809 nm emission route.

  • Widefield Dichroic
    Dichroic
    Element type: Fixed
    Pos 1: Multiband Dichroic (FF410/504/582/669-Di01-25x36) — emission 440/25 nm, 521/25 nm, 607/25 nm, 700/25 nm

  • CSU-W1 Emission Wheel
    Emission
    Element type: Filter Wheel
    Pos 1: Quad-band emitter (FF01-440/521/607/700-25) — emission 440/25 nm, 521/25 nm, 607/25 nm, 700/25 nm — Quad-band emitter for multicolor spinning-disk imaging
    Pos 2: DAPI (FF01-445/45-25) — emission 445/45 nm
    Pos 3: GFP — emission 525/50 nm — Previously 525/30 (FF01-525/30-25 in the old PDF) before 2021-08-18.
    Pos 4: Cy3 / Alexa 568 (FF01-617/73-25) — emission 617/73 nm
    Pos 5: Cy5 / Alexa 647 (FF01-692/40-25) — emission 692/40 nm
    Pos 6: Alexa 750 (FF02-809/81-25) — emission 809/81 nm

  • Widefield Emission
    Emission
    Element type: Fixed
    Pos 1: Multiband Bandpass (FF01-440/521/607/700-25) — emission 440/25 nm, 521/25 nm, 607/25 nm, 700/25 nm

QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data

  • Plan-Apochromat 10x/0.45 Ph1 M27 10x/0.45 AIR (10x_air)

  • Plan-Apochromat 20x/0.8 20x/0.8 AIR (20x_air)

  • LD C-APOCHROMAT 40x/1.1 W M27 40x/1.1 WATER (40x_water)

  • Plan-Apochromat 63x/1.4 Oil DIC M27 63x/1.4 OIL (63x_oil)

  • Plan-Apochromat 100x/1.4 Oil DIC M27 100x/1.4 OIL (100x_oil)

  • LD LCI Plan-APOCHROMAT 25x/0.8 Imm Korr DIC (UV)VIS-IR 25x/0.8 MULTI-IMMERSION (25x_multi)

  • C-APOCHROMAT 63x/1.2 W Korr UV-VIS-IR 63x/1.2 WATER (63x_water_c_apo)

Metadata Completeness

  • Legacy aliases in use (fallback accepted):

  • modules[].name → modules[].type

  • hardware.detectors[].pixel_size_um → hardware.detectors[].pixel_pitch_um

  • hardware.detectors[].min_nm → hardware.detectors[].collection_min_nm

  • hardware.detectors[].max_nm → hardware.detectors[].collection_max_nm

  • hardware.detectors[].channel_center_nm → hardware.detectors[].collection_center_nm

  • hardware.detectors[].bandwidth_nm → hardware.detectors[].collection_width_nm