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3i CSU-W1 Spinning Disk

About this microscope

Here you’ll find the hardware and capabilities currently recorded for this microscope. If you are planning an experiment, AIC Turku staff can help with access, training, and configuration choices.

Current status: 🟢 Online

Operational

QC and maintenance history Open Virtual Microscope

3i CSU-W1 Spinning Disk microscope
Manufacturer
3i / Zeiss
Model
Marianas CSU-W1 Spinning Disk Confocal
Stand
Inverted
Eyepieces
Trinocular
Location
BioCity, 5th floor, room 5149
Purchased
2015
Funding
—
Capabilities
These are the capabilities recorded for this microscope. Open the optical-route details below to see how the hardware is connected.
Confocal spinning diskWidefield fluorescenceTransmitted brightfieldPhase contrastDICLive cell imagingFRAPPhotoactivation
Methods and the light path that records them
Each method below is explicitly associated in the instrument record with the physical light path that implements it. An imaging mode or contrast method that appears above but not here has no recorded method-to-path mapping yet.
  • Spinning-disk confocal: Confocal spinning disk
  • Widefield fluorescence: Widefield fluorescence
  • Transmitted light: Transmitted brightfield, Phase contrast, DIC
Modules
FRAP Module Piezo Z-Stage Hardware Autofocus Incubation


Software

  • 3i SlideBook (v2026.1 (49301))
    Acquisition
    Role: Acquisition
    Version: 2026.1 (49301)

Hardware

Environmental control

  • Environmental Control
    controlled temperature, controlled CO2
    Temperature control: Yes
    CO2 control: Yes
    O2 control: No
    Notes: Okolab Full Enclosure Incubator with temperature and digital CO2 control

Light sources

  • 405 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 405 nm
    Power: 100 mW
    Notes: Visible LaserStack line routed through the CSU-W VIS input and dichroic (filter-turret position 1).

  • 488 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 488 nm
    Power: 150 mW
    Notes: Visible LaserStack line routed through the CSU-W VIS input (filter-turret position 1).

  • 561 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 561 nm
    Power: 100 mW
    Notes: Visible LaserStack line routed through the CSU-W VIS input (filter-turret position 1).

  • 640 nm Laser 3i LaserStack v4
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 640 nm
    Power: 100 mW
    Notes: Visible LaserStack line routed through the CSU-W VIS input (filter-turret position 1).

  • 730 nm Laser 3i SingleLine 730nm Laser Launch
    3i
    Type: Laser
    Role: Excitation
    Wavelength: 730 nm
    Power: 30 mW
    Notes: Near-IR excitation delivered through the dedicated CSU-W1 NIR input, via the installed Yokogawa DM A660LP beam combiner, before joining the common CSU-W path (filter-turret position 2). NIR Shift is position 2 for ORCA and position 1 for Evolve.

  • LED Excelitas X-Cite XLED1
    Excelitas
    Type: LED
    Role: Excitation
    Notes: 4-channel LED light source for DAPI, FITC, TRITC/Texas Red, and Cy5-class widefield fluorescence

Scanner

  • Spinning Disk Scanner
    Yokogawa CSU-W1
    Type: Spinning Disk Scanner
    Manufacturer: Yokogawa
    Model: CSU-W1
    Pinhole: 50 µm
    Notes: 50 µm pinhole disk. Appropriate for standard live-cell spinning-disk confocal imaging; less optimal than a 25 µm disk for low-magnification confocal sectioning.

Detectors

  • Hamamatsu ORCA-Flash4.0
    Scientific CMOS
    Type: Scientific CMOS
    Pixel pitch: 6.5 µm
    Sensor format: 2048 x 2048
    QE peak: 82%
    Notes: SlideBook Camera 1. In the 730 nm configuration, the Yokogawa CSU-W Camera Switcher is at position 1 and NIR Shift is at position 2.

  • Photometrics Evolve
    Electron-Multiplying CCD
    Type: Electron-Multiplying CCD
    Pixel pitch: 16 µm
    Sensor format: 512 x 512
    QE peak: 95%
    Notes: 10 MHz back-illuminated EMCCD. SlideBook Camera 2. In the 730 nm configuration, the Yokogawa CSU-W Camera Switcher is at position 2 and NIR Shift is at position 1.

Objectives

View this microscope's objectives in the catalogue

  • Plan-Apochromat 10x/0.45 Ph1 M27 AIR
    Zeiss
    Model: Plan-Apochromat 10x/0.45 Ph1 M27
    Magnification / NA: 10x/0.45
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 2.0 mm
    Product code: 420641-9910
    AFC compatible: No
    Installed: Yes
    Specialties: phase

  • Plan-Apochromat 20x/0.8 AIR
    Zeiss
    Model: Plan-Apochromat 20x/0.8
    Magnification / NA: 20x/0.8
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 0.55 mm
    Product code: 440640-9903-000
    AFC compatible: No
    Installed: Yes

  • LD C-APOCHROMAT 40x/1.1 W M27 WATER
    Zeiss
    Model: LD C-APOCHROMAT 40x/1.1 W M27
    Magnification / NA: 40x/1.1
    Immersion: Water
    Correction: Apochromat
    Working distance: 0.62 mm
    Product code: 421867-9970
    AFC compatible: No
    Installed: Yes
    Specialties: coverslip_correction, ir_transmission

  • Plan-Apochromat 63x/1.4 Oil DIC M27 OIL
    Zeiss
    Model: Plan-Apochromat 63x/1.4 Oil DIC M27
    Magnification / NA: 63x/1.4
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.19 mm
    Product code: 420782-9900
    AFC compatible: No
    Installed: Yes
    Specialties: dic

  • Plan-Apochromat 100x/1.4 Oil DIC M27 OIL
    Zeiss
    Model: Plan-Apochromat 100x/1.4 Oil DIC M27
    Magnification / NA: 100x/1.4
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.17 mm
    Product code: 420792-9901
    AFC compatible: No
    Installed: Yes
    Specialties: dic

  • LD LCI Plan-APOCHROMAT 25x/0.8 Imm Korr DIC (UV)VIS-IR MULTI-IMMERSIONNot installed
    Zeiss
    Model: LD LCI Plan-APOCHROMAT 25x/0.8 Imm Korr DIC (UV)VIS-IR
    Magnification / NA: 25x/0.8
    Immersion: Multi-Immersion
    Correction: Plan Apochromat
    Working distance: 0.57 mm at cover glass 0.17
    Product code: 420852-9871
    AFC compatible: No
    Installed: No
    Specialties: dic, ir_transmission, correction_collar
    Notes: Optional objective. Multi-immersion correction-collar objective.
  • C-APOCHROMAT 63x/1.2 W Korr UV-VIS-IR WATERNot installed
    Zeiss
    Model: C-APOCHROMAT 63x/1.2 W Korr UV-VIS-IR
    Magnification / NA: 63x/1.2
    Immersion: Water
    Correction: Apochromat
    Working distance: 0.28 mm at cover glass 0.17
    Product code: 421787-9970
    AFC compatible: No
    Installed: No
    Specialties: ir_transmission, correction_collar
    Notes: Optional objective. Water-immersion correction-collar objective.

Stages and focus drives

  • Z Piezo Stage — ASI PZ-2000
    ASI
    Type: Z Piezo Stage

Autofocus and triggering

  • Infrared Reflection Autofocus
    Installed: Yes
    Type: Infrared Reflection Autofocus

Optical-path elements

  • XLED Excitation Filters
    Excitation
    Element type: Filter Wheel
    Pos 1: Bandpass (FF01-387/11-25) — emission 387/11 nm
    Pos 2: Bandpass (FF02-485/20-25) — emission 485/20 nm
    Pos 3: Bandpass (FF01-560/25-25) — emission 560/25 nm
    Pos 4: Bandpass (FF01-650/13-25) — emission 650/13 nm

  • Yokogawa CSU-W Filter Turret
    Dichroic
    Element type: Turret
    Pos 1: VIS quad-band confocal dichroic (Di01-T405/488/568/647) — Laser-optimized confocal dichroic for the 405/488/561/640 LaserStack route (filter-turret position 1). Semrock specifies transmission at the laser lines (405, 488, 561-568, 638-647 nm) and reflection at 422-473, 503-545, 586-620, 665-750 nm; not recorded as generic pass bands here, since that would misrepresent this dichroic's reflection-side behavior in the simulator.
    Pos 2: NIR short-pass confocal dichroic — Filter-turret position 2, used by both the 730 ORCA and 730 Evolve configurations. Identified locally as Chroma T750SPXR-XT-UF1; no verified transmission/reflection curve on file for this ultra-flat optic.

  • CSU-W NIR Beam Combiner
    Dichroic
    Element type: Fixed
    Details: Source-specific optic on the dedicated 730 nm NIR input before it joins the common CSU-W path; the 405/488/561/640 LaserStack lines enter through the VIS input and do not traverse this optic.
    Pos 1: NIR beam combiner — Identity documented locally; no verified spectral curve or catalogue number on file.

  • Widefield Dichroic
    Dichroic
    Element type: Fixed
    Pos 1: Multiband Dichroic (FF410/504/582/669-Di01-25x36) — emission 440/40 nm, 520.5/21 nm, 606.5/34 nm, 699.5/45 nm — Semrock transmission windows: 420-460, 510-531, 589.5-623.5, 677-722 nm.

  • Yokogawa CSU-W Emission Filter Wheel 1
    Emission
    Element type: Filter Wheel
    Details: First camera-port emission wheel (10-position, per Yokogawa spec for the two-camera CSU-W1). SlideBook records both wheels as one two-digit code, e.g. 488 ORCA = 31 and 730 ORCA = 61 (wheel-1 positions 3 and 6).
    Pos 1: Quad-band emitter (FF01-440/521/607/700-25) — emission 440/40 nm, 521/21 nm, 607/34 nm, 700/45 nm — Quad-band emitter for multicolor spinning-disk imaging.
    Pos 2: DAPI (FF01-445/45-25) — emission 445/45 nm
    Pos 3: GFP — emission 525/50 nm — SlideBook 488 ORCA configuration selects wheel-1 position 3. Previously 525/30 (FF01-525/30-25) before 2021-08-18.
    Pos 4: Cy3 / Alexa 568 (FF01-617/73-25) — emission 617/73 nm
    Pos 5: Cy5 / Alexa 647 (FF01-692/40-25) — emission 692/40 nm
    Pos 6: Alexa 750 (FF02-809/81-25) — emission 809/81 nm — SlideBook 730 ORCA configuration selects wheel-1 position 6.

  • Yokogawa CSU-W Emission Filter Wheel 2
    Emission
    Element type: Filter Wheel
    Details: Second camera-port emission wheel (6-position, per Yokogawa spec for the two-camera CSU-W1). SlideBook's 730 Evolve code is 16 (wheel-1 position 1, wheel-2 position 6). Other wheel-2 positions are unverified and not copied from wheel 1.
    Pos 6: Alexa 750 (FF02-809/81-25) — emission 809/81 nm

  • Widefield Emission
    Emission
    Element type: Fixed
    Pos 1: Multiband Bandpass (FF01-440/521/607/700-25) — emission 440/40 nm, 521/21 nm, 607/34 nm, 700/45 nm

Latest QC measurements

Laser performance

  • 405 nm laser power at fiber output sdc

    20 mW
    laser.fiber_output_sdc.405.power_mw

  • 405 nm laser power at fiber output vector

    60 mW
    laser.fiber_output_vector.405.power_mw

  • 488 nm laser power at fiber output sdc

    37 mW
    laser.fiber_output_sdc.488.power_mw

  • 488 nm laser power at fiber output vector

    78 mW
    laser.fiber_output_vector.488.power_mw

  • 561 nm laser power at fiber output sdc

    37 mW
    laser.fiber_output_sdc.561.power_mw

  • 561 nm laser power at fiber output vector

    24 mW
    laser.fiber_output_vector.561.power_mw

  • 640 nm laser power at fiber output sdc

    19 mW
    laser.fiber_output_sdc.640.power_mw

  • 640 nm laser power at fiber output vector

    5 mW
    laser.fiber_output_vector.640.power_mw

  • 730 nm laser power at fiber output sdc

    21 mW
    laser.fiber_output_sdc.730.power_mw

Optical resolution (PSF and alignment)

No recent PSF or chromatic-shift measurements are recorded for these objectives.

Objectives without recent measurements

  • Plan-Apochromat 10x/0.45 Ph1 M27 AIR (10x_air)

  • Plan-Apochromat 20x/0.8 AIR (20x_air)

  • LD C-APOCHROMAT 40x/1.1 W M27 WATER (40x_water)

  • Plan-Apochromat 63x/1.4 Oil DIC M27 OIL (63x_oil)

  • Plan-Apochromat 100x/1.4 Oil DIC M27 OIL (100x_oil)

  • LD LCI Plan-APOCHROMAT 25x/0.8 Imm Korr DIC (UV)VIS-IR MULTI-IMMERSION (25x_multi)

  • C-APOCHROMAT 63x/1.2 W Korr UV-VIS-IR WATER (63x_water_c_apo)