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Leica STELLARIS 8 FALCON FLIM

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Current Status: 🟢 Online

Operational

📊 View QC & Maintenance History 🔬 Open Virtual Microscope

Leica STELLARIS 8 FALCON FLIM
Manufacturer
Leica Microsystems
Model
STELLARIS 8 FALCON
Stand
Inverted
Eyepieces
Trinocular
Location
BioCity, 5th floor, room 5149
Purchased
2023
Funding
unknown
Capabilities
Use these chips to quickly decide if this microscope supports your planned imaging mode, contrast method, or readout.
Confocal point scanningWidefield fluorescenceTransmitted brightfieldSpectral ImagingFLIMFCSFRETLive cell imaging
Modules
FLIM Module FCS Module Acousto-Optical Beam Splitter (AOBS) Hardware Autofocus Motorized Stage Incubation High-Performance Workstation


Software Environment

  • LAS X STELLARIS Control Software
    Acquisition
    Role: Acquisition
    Version: unknown
    Notes: Product code 158203200. The exact installed LAS X version must be read from The acquisition workstation.

  • LAS X Dye Finder
    Acquisition
    Role: Acquisition
    Version: unknown
    Notes: Product code 158203206.

  • LAS X Assay Editor
    Acquisition
    Role: Acquisition
    Version: unknown
    Notes: Product code 158203211.

  • LAS X 3D Visualisation
    Analysis
    Role: Analysis
    Version: unknown
    Notes: Product code 158203202.

  • LAS X Live Data Mode
    Acquisition
    Role: Acquisition
    Version: unknown
    Notes: Product code 158203201.

  • LAS X AiviaMotion
    Analysis
    Role: Analysis
    Version: unknown
    Notes: Product code 158203220.

  • LAS X MicroLab
    Acquisition
    Role: Acquisition
    Version: unknown
    Notes: Product code 158203207.

  • LAS X Lightning Expert
    Processing
    Role: Processing
    Version: unknown
    Notes: Product code 158203204.

  • LAS X Environmental Control
    Hardware Control
    Role: Hardware Control
    Version: unknown
    Notes: Product code 158203214.

  • LAS X water pump
    Hardware Control
    Role: Hardware Control
    Version: unknown
    Notes: Product code 158203218.

Hardware Configuration

🌡️ Environmental Control

  • Environmental Control
    controlled temperature, controlled CO2
    Temperature control: Yes
    CO2 control: Yes
    Humidity control: No
    Notes: Okolab Black Box Incubator DMi8, Okolab Sample Chamber Inv Super Z, and Okolab CO2, Humidity Passive are listed in the vendor quotation. Controlled via LAS X Environmental Control. Exact temperature, CO2, and humidity operating ranges are not documented in the provided records.

💡 Light Sources

  • White Light Laser Leica Microsystems STELLARIS White Light Laser
    Leica Microsystems
    Type: White Light Laser
    Technology: supercontinuum
    Power: unknown
    Notes: Local quotation states visible excitation range 440-790 nm. Service report SA-223726 records WLL replacement and that all WLL laser lines were in specification after integration. Measured powers are not provided.

  • 405 nm Laser Leica Microsystems Laser 405 DMOD
    Leica Microsystems
    Type: Laser
    Technology: diode
    Wavelength: 405 nm
    Power: unknown
    Product code: 158202140
    Notes: Local quotation also lists Laser Port 405/UV, product code 158301200.

  • LED Leica Microsystems Leica LED 3
    Leica Microsystems
    Type: LED
    Technology: led_array
    Power: unknown
    Product code: 158000677
    Notes: Used with installed observation filter cubes and transmitted/stand observation workflows. Exact LED channels/powers are not documented in the provided records.

🧭 Scanner

  • Tandem Scanner (Galvo/Resonant)
    Type: Tandem Scanner (Galvo/Resonant)
    Line rate: 8000 Hz
    Notes: Product code 158301131: 8 kHz Tandem Scanner STELLARIS 8. Local quotation describes a tandem scanning system switchable between resonant and non-resonant/galvanometric mode.

📷 Detectors

  • Leica Microsystems Power HyD S SP pos 1
    Hybrid Detector
    Type: Hybrid Detector
    Supports time gating: No
    QE peak: 56%
    Product code: 158301311
    Notes: Installed the spectral detector at position 1. Leica describes Power HyD S as an all-round detector with high performance throughout the spectrum and enhanced blue-green photon detection efficiency. PDE/QE metadata is catalogue/family-level, not a local measurement.

  • Leica Microsystems Power HyD X SP pos 2
    Hybrid Detector
    Type: Hybrid Detector
    Supports time gating: Yes
    QE peak: 46%
    Product code: 158301322
    Notes: Installed the spectral detector at position 2. FALCON/FCS-capable detector for visible to far-red workflows. QE/range are catalogue/facility-spec values, not local measurements. Do not treat time-gating delay/width as Fixed defaults; they are experiment/runtime settings.

  • Leica Microsystems Power HyD S SP Core Unit pos 3
    Hybrid Detector
    Type: Hybrid Detector
    Supports time gating: No
    QE peak: 56%
    Product code: 158301313
    Notes: Installed the spectral detector position 3 / SP Core Unit. PDE/QE metadata It is a catalogue/family-level measurement, not a local one.

  • Leica Microsystems Power HyD X SP pos 4
    Hybrid Detector
    Type: Hybrid Detector
    Supports time gating: Yes
    QE peak: 46%
    Product code: 158301324
    Notes: Installed the spectral detector at position 4. FALCON/FCS-capable detector for visible to far-red workflows. QE/range are catalogue/facility-spec values, not local measurements. Do not treat time-gating delay/width as Fixed defaults; they are experiment/runtime settings.

  • Leica Microsystems Power HyD R SP pos 5
    Hybrid Detector
    Type: Hybrid Detector
    Supports time gating: Yes
    QE peak: 26%
    Product code: 158301335
    Notes: Installed the spectral detector at position 5. Near-infrared FALCON/FCS detector. QE/range are catalogue/facility-spec values, not local measurements. Do not treat time-gating delay/width as fixed defaults; They are experiment/runtime settings.

  • Leica Microsystems BF detector for DMI
    Photomultiplier Tube
    Type: Photomultiplier Tube
    QE peak: 30%
    Product code: 158004201
    Notes: Transmitted-light / brightfield detector listed in local quotation. QE is a generic placeholder needed by the current confocal detector-completeness policy, not a measured local value.

  • Leica Microsystems K5 Microscope Camera
    Scientific CMOS
    Type: Scientific CMOS
    Pixel pitch: 6.5 µm
    Sensor format: 4.2 MP
    QE peak: 80%
    Product code: 11547112
    Notes: External/stand camera via C-mount adapter 1x HC f. 1, product code 11541510. Camera specs are online catalogue values; this is not the default detector for confocal spectral scanning.

🔬 Objectives

  • HC PL APO 10x/0.40 CS2 10x/0.4 AIR
    Leica Microsystems
    Model: HC PL APO 10x/0.40 CS2
    Magnification / NA: 10x/0.4
    Immersion: Air
    Correction: Apochromat
    Working distance: unknown
    Product code: 15506424
    AFC compatible: Yes
    Installed: Yes
    Notes: Installed objective listed in local quotation.

  • HC PL APO 20x/0.75 CS2 20x/0.75 AIR
    Leica Microsystems
    Model: HC PL APO 20x/0.75 CS2
    Magnification / NA: 20x/0.75
    Immersion: Air
    Correction: Apochromat
    Working distance: unknown
    Product code: 15506517
    AFC compatible: Yes
    Installed: Yes
    Notes: Installed objective listed in local quotation.

  • HC PL APO 40x/1.25 GLYC CORR CS2 40x/1.25 GLYCEROL
    Leica Microsystems
    Model: HC PL APO 40x/1.25 GLYC CORR CS2
    Magnification / NA: 40x/1.25
    Immersion: Glycerol
    Correction: Apochromat
    Working distance: unknown
    Product code: 15506422
    AFC compatible: Yes
    Installed: Yes
    Specialties: correction_collar
    Notes: Installed objective listed in local quotation. Uses Type G immersion liquid, product code 15513910.

  • HC PL APO 63x/1.40 OIL CS2 63x/1.4 OIL
    Leica Microsystems
    Model: HC PL APO 63x/1.40 OIL CS2
    Magnification / NA: 63x/1.4
    Immersion: Oil
    Correction: Apochromat
    Working distance: unknown
    Product code: 15506350
    AFC compatible: Yes
    Installed: Yes
    Notes: Installed objective listed in local quotation. Uses Type F immersion liquid, ISO 8036, product code 15513859.

  • HC PL APO 86x/1.20 W motCORR STED W 86x/1.2 WATER
    Leica Microsystems
    Model: HC PL APO 86x/1.20 W motCORR STED W
    Magnification / NA: 86x/1.2
    Immersion: Water
    Correction: Apochromat
    Working distance: unknown
    Product code: 15506333
    AFC compatible: Yes
    Installed: Yes
    Specialties: correction_collar, sted
    Notes: Installed water objective listed in local quotation. Associated motCORR board product code 15525212 and water immersion micro dispenser product code 15640019. Included because it is installed, even though this instrument record does not currently declare a STED modality.

🧭 Stages & Focus Drives

  • Motorized XY Stage — Leica Microsystems Scanning stage inv. universal
    Leica Microsystems
    Type: Motorized XY Stage

  • Z Piezo Stage — Leica Microsystems SuperZ Galvo Stage
    Leica Microsystems
    Type: Z Piezo Stage

⚙️ Advanced Hardware

  • Infrared Reflection Autofocus
    Installed: Yes
    Type: Infrared Reflection Autofocus

🎛️ Optical Path Elements

  • Acousto-Optical Beam Splitter
    Dichroic
    Element type: Splitter
    Details: Installed AOBS for software-controlled wavelength selection/routing.

  • Prism-based spectral detection unit
    Emission
    Element type: Spectral Slider
    Details: Computer-controlled adjustable emission bandwidth. Local quotation states detection range up to 850 nm and up to five simultaneous fluorescence channels.

  • Reflected-light filter cube turret
    Cube
    Element type: Turret
    Cy5 Narrow Cube: Filter Cube (11536078) — Catalogue-derived Cy5 narrow bands from product listing; verify against installed cube label if exact bands matter.
    Gfp Cube: Filter Cube (15525314) — Catalogue-derived GFP cube bands; verify against installed cube label if exact bands matter.
    Icr Reflector Cube: Analyzer (11525375) — Reflector cube with 45° dichroic mirror and a permanently installed and fixed crossed polariser and analyser. This is not an authoritative fluorescence filter cube and should not be used for spectral fluorophore simulation.
    Led 405 Cube: Filter Cube (15525338) — Spectral bands are catalogue-derived from matching Leica-style filter-cube references; verify against installed cube label before treating as measured local truth.
    Txr Cube: Filter Cube (15525310) — Catalogue-derived TXR cube bands; verify against installed cube label if exact bands matter.

  • Transmitted-light / BF path
    Excitation
    Element type: Fixed
    Details: Includes BF detector for DMI and transmitted-light observation path.

QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data

  • HC PL APO 10x/0.40 CS2 10x/0.4 AIR (obj_10x_air_0_40)

  • HC PL APO 20x/0.75 CS2 20x/0.75 AIR (obj_20x_air_0_75)

  • HC PL APO 40x/1.25 GLYC CORR CS2 40x/1.25 GLYCEROL (obj_40x_glycerol_1_25_corr)

  • HC PL APO 63x/1.40 OIL CS2 63x/1.4 OIL (obj_63x_oil_1_40)

  • HC PL APO 86x/1.20 W motCORR STED W 86x/1.2 WATER (obj_86x_water_1_20_motcorr_sted)