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MSquared Aurora Airy Beam

About this microscope

Here you’ll find the hardware and capabilities currently recorded for this microscope. If you are planning an experiment, AIC Turku staff can help with access, training, and configuration choices.

Current status: 🟢 Online

Operational

QC and maintenance history Open Virtual Microscope

MSquared Aurora Airy Beam microscope
Manufacturer
M Squared
Model
Aurora Airy Beam
Stand
Other
Eyepieces
Camera-only
Location
BioCity, 5th floor, room 5149
Purchased
2019
Funding
—
Capabilities
These are the capabilities recorded for this microscope. Open the optical-route details below to see how the hardware is connected.
Light sheetLive cell imaging
Methods and the light path that records them
Each method below is explicitly associated in the instrument record with the physical light path that implements it. An imaging mode or contrast method that appears above but not here has no recorded method-to-path mapping yet.
  • Light sheet: Light sheet
Modules
Environmental Enclosure


Software

  • M Squared Cubes Acquisition
    Acquisition
    Role: Acquisition
    Version: Not documented

  • M Squared Cubes Deconvolution
    Processing
    Role: Processing
    Version: Not documented

Hardware

Environmental control

  • Environmental Control
    controlled temperature
    Temperature control: Yes

Light sources

  • 488 nm Laser Coherent OBIS 488 nm laser
    Coherent OBIS
    Type: Laser
    Wavelength: 488 nm
    Power: 100 mW

  • 561 nm Laser Coherent OBIS 561 nm laser
    Coherent OBIS
    Type: Laser
    Wavelength: 561 nm
    Power: 80 mW

  • 640 nm Laser Coherent OBIS 640 nm laser (LX)
    Coherent OBIS
    Type: Laser
    Wavelength: 640 nm
    Power: 75 mW
    Notes: FIBER PIGTAIL:UFC: Galaxy

Scanner

  • Tandem Scanner (Galvo/Resonant)
    Type: Tandem Scanner (Galvo/Resonant)
    Light-sheet type: airy_beam
    Line rate: 8000 Hz
    Notes: Galvanometric scanner and remote focusing lenses on illumination/detection; integrated Resonant scanner for beam scanning.

Detectors

  • Hamamatsu ORCA-Flash4.0 V3 (C11440-22CU)
    Scientific CMOS
    Type: Scientific CMOS
    Pixel pitch: 6.5 µm
    Sensor format: 2048 x 2048
    QE peak: 82%
    Notes: Supplied with water chiller for camera chip cooling

Objectives

View this microscope's objectives in the catalogue

  • 54-10-12 Airy beam dipping objective 17x/0.4 MULTI-IMMERSION
    Special Optics
    Model: 54-10-12 Airy beam dipping objective
    Magnification / NA: 17x/0.4
    Immersion: Multi-Immersion
    Correction: Fluorite
    Working distance: 12 mm
    Product code: 54-10-12
    AFC compatible: No
    Installed: Yes
    Specialties: cleared_tissue_imaging
    Notes: Excitation and detection objectives designed for dipping media with refractive index range 1.33-1.56 (NA 0.37-0.43), magnification range 15.3x-17.9x across RI. FOV 1.2 mm diameter.

Optical-path elements

  • Emission Wheel
    Emission
    Element type: Filter Wheel
    Pos 1: GFP filter — emission 520/40 nm
    Pos 2: GFP longpass
    Pos 3: RFP longpass

Latest QC measurements

Laser performance

No recent laser-performance measurements are recorded.

Optical resolution (PSF and alignment)

No recent PSF or chromatic-shift measurements are recorded for these objectives.

Objectives without recent measurements
  • 54-10-12 Airy beam dipping objective 17x/0.4 MULTI-IMMERSION (17x_multi)

Information still to confirm

Some details in the facility record are incomplete. If any of these matter for your experiment, please check them with AIC Turku staff.

  • Details needed for this configuration:

  • Software version (software[].version)