Zeiss TIRF
Work in progress
This dashboard is a work in progress. Please independently verify critical information, as not all features are working yet.
Current Status: 🟡 Warning
Limited to widefield imaging only
Software Environment
- Unknown
Acquisition
Role: Acquisition
Version: Not documented
Hardware Configuration
🌡️ Environmental Control
- Environmental Control
controlled temperature
Temperature control: Yes
Notes: Heated incubator and sample holder
💡 Light Sources
-
488 nm Laser Unknown
Unknown
Type: Laser
Wavelength:488 nm -
561 nm Laser Unknown
Unknown
Type: Laser
Wavelength:561 nm -
639 nm Laser Unknown
Unknown
Type: Laser
Wavelength:639 nm -
Halogen Lamp Zeiss Transmitted Halogen Lamp
Zeiss
Type: Halogen Lamp
Role: Transmitted Illumination
Notes: Transmitted source for brightfield.
📷 Detectors
-
Hamamatsu C9100-13
Electron-Multiplying CCD
Type: Electron-Multiplying CCD
Pixel pitch:16 µm
Sensor format:512 x 512 -
Hamamatsu ORCA-Flash4.0 CMOS
Scientific CMOS
Type: Scientific CMOS
Pixel pitch:6.5 µm
Sensor format:2048 x 2048
🔬 Objectives
-
EC Epiplan-Neofluar 2.5x/0.06 AIR
Zeiss
Model: EC Epiplan-Neofluar
Magnification / NA:2.5x/0.06
Immersion: Air
Correction: Plan Fluorite
Working distance:15.1 mm
AFC compatible: No
Installed: Yes -
EC Epiplan-Neofluar 10x/0.3 AIR
Zeiss
Model: EC Epiplan-Neofluar
Magnification / NA:10x/0.3
Immersion: Air
Correction: Plan Fluorite
Working distance:5.2 mm
AFC compatible: No
Installed: Yes -
Plan Apochromat 20x/0.45 AIR
Zeiss
Model: Plan Apochromat
Magnification / NA:20x/0.45
Immersion: Air
Correction: Plan Apochromat
Working distance:8.2-6.9 mm
AFC compatible: Yes
Installed: Yes -
Alpha Plan Apochromat 63x/1.46 OIL
Zeiss
Model: Alpha Plan Apochromat
Magnification / NA:63x/1.46
Immersion: Oil
Correction: Plan Apochromat
Working distance:0.55 mm
AFC compatible: Yes
Installed: Yes
Specialties: tirf -
Alpha Plan Apochromat 100x/1.46 OIL
Zeiss
Model: Alpha Plan Apochromat
Magnification / NA:100x/1.46
Immersion: Oil
Correction: Plan Apochromat
Working distance:0.11 mm
AFC compatible: Yes
Installed: Yes
Specialties: tirf
🧭 Stages & Focus Drives
- Motorized XY Stage — Zeiss Motorized XY Stage
Zeiss
Type: Motorized XY Stage
⚙️ Advanced Hardware
- Infrared Reflection Autofocus
Installed: Yes
Type: Infrared Reflection Autofocus
🎛️ Optical Path Elements
- Filter Turret
Cube
Element type: Turret
Pos 1: Filter set 38 HE (GFP) (38 HE) — emission 525/50 nm — Excitation: 470/40 nm.
Pos 2: Filter set 43 HE (DsRed) (43 HE) — emission 605/70 nm — Excitation: 550/25 nm.
Pos 3: Filter set 76 HE (CFP/GFP/DsRed) (76 HE) — emission 459/25 nm, 525/30 nm, 608/30 nm — Excitation: TBP 406+489+561 nm. Emission: TBP 459+525+608 nm.
Pos 4: Filter set 77 HE (GFP/mRFP/Alexa 633) (77 HE) — emission 526/25 nm, 601/30 nm, 688/40 nm — Excitation: TBP 483+564+642 nm. Emission: TBP 526+601+688 nm.
QC Metrics: Latest Measurements
🔦 Laser Performance
No laser performance metrics recorded recently.
🔬 Optical Resolution (PSF & Alignment)
No recent PSF or chromatic shift metrics available for any objectives.
Objectives without recent data
-
EC Epiplan-Neofluar 2.5x/0.06 AIR (
2.5x_air) -
EC Epiplan-Neofluar 10x/0.3 AIR (
10x_air) -
Plan Apochromat 20x/0.45 AIR (
20x_air) -
Alpha Plan Apochromat 63x/1.46 OIL (
63x_oil) -
Alpha Plan Apochromat 100x/1.46 OIL (
100x_oil)
Metadata Completeness
-
Missing conditional fields (currently applicable):
-
software[].version— Software version