Zeiss TIRF

About this microscope

Here you’ll find the hardware and capabilities currently recorded for this microscope. If you are planning an experiment, AIC Turku staff can help with access, training, and configuration choices.

Current status: 🟡 Warning

Limited to widefield imaging only

QC and maintenance history Open Virtual Microscope

Manufacturer

Zeiss

Model

TIRF

Stand

Inverted

Eyepieces

Trinocular

Location

BioCity, 2nd floor, room 2209

Purchased

—

Funding

—

Capabilities

These are the capabilities recorded for this microscope. Open the optical-route details below to see how the hardware is connected.

Widefield fluorescenceTIRFTransmitted brightfieldLive cell imaging

Methods and the light path that records them

Each method below is explicitly associated in the instrument record with the physical light path that implements it. An imaging mode or contrast method that appears above but not here has no recorded method-to-path mapping yet.

• Widefield fluorescence: Widefield fluorescence, TIRF
• Transmitted light: Transmitted brightfield

Modules

Incubation Hardware Autofocus Motorized Stage

Software

• Unknown
  Acquisition
  Role: Acquisition
  Version: Not documented

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Hardware

Environmental control

• Environmental Control
  controlled temperature
  Temperature control: Yes
  Notes: Heated incubator and sample holder

Light sources

• 488 nm Laser Unknown
  Unknown
  Type: Laser
  Wavelength: 488 nm

• 561 nm Laser Unknown
  Unknown
  Type: Laser
  Wavelength: 561 nm

• 639 nm Laser Unknown
  Unknown
  Type: Laser
  Wavelength: 639 nm

• Halogen Lamp Zeiss Transmitted Halogen Lamp
  Zeiss
  Type: Halogen Lamp
  Role: Transmitted Illumination
  Notes: Transmitted source for brightfield.

Detectors

• Hamamatsu C9100-13
  Electron-Multiplying CCD
  Type: Electron-Multiplying CCD
  Pixel pitch: 16 µm
  Sensor format: 512 x 512

• Hamamatsu ORCA-Flash4.0 CMOS
  Scientific CMOS
  Type: Scientific CMOS
  Pixel pitch: 6.5 µm
  Sensor format: 2048 x 2048

Objectives

View this microscope's objectives in the catalogue

• EC Epiplan-Neofluar 2.5x/0.06 AIR
  Zeiss
  Model: EC Epiplan-Neofluar
  Magnification / NA: 2.5x/0.06
  Immersion: Air
  Correction: Plan Fluorite
  Working distance: 15.1 mm
  AFC compatible: No
  Installed: Yes
  Notes: Pre-existing ledger record, kept as installed. The current facility OpenIRIS objective list (10x/20x/40x/63x/100x) does not include a 2.5x objective; whether it is still on the microscope is an open staff question, not a confirmed removal.

• EC Plan-Neofluar 10x/0.3 AIR
  Zeiss
  Model: EC Plan-Neofluar 10x/0.3
  Magnification / NA: 10x/0.3
  Immersion: Air
  Correction: Plan Fluorite
  Working distance: 5.2 mm
  Product code: 420340-9901
  Installed: Yes

• Plan-Apochromat 20x/0.8 AIR
  Zeiss
  Model: Plan-Apochromat 20x/0.8
  Magnification / NA: 20x/0.8
  Immersion: Air
  Correction: Plan Apochromat
  Working distance: 0.55 mm
  Product code: 420650-9901
  Installed: Yes

• LD Achroplan 40x/0.60 Korr Ph2 AIR
  Zeiss
  Model: LD Achroplan 40x/0.60 Korr Ph2
  Magnification / NA: 40x/0.6
  Immersion: Air
  Correction: Plan Achromat
  Working distance: 1.8 mm at cover glass 1.0
  Product code: 440865
  Installed: Yes
  Specialties: phase, correction_collar
  Notes: Objective listed for this microscope in the facility OpenIRIS record; working distance cross-checked against ZEISS Axiovert 200 documentation.

• alpha Plan-Apochromat 63x/1.46 Oil Corr TIRF OIL
  Zeiss
  Model: alpha Plan-Apochromat 63x/1.46 Oil Corr TIRF
  Magnification / NA: 63x/1.46
  Immersion: Oil
  Correction: Plan Apochromat
  Working distance: 0.10 mm
  Product code: 420780-9970
  Installed: Yes
  Specialties: tirf

• alpha Plan-Apochromat 100x/1.46 Oil DIC (UV) VIS-IR OIL
  Zeiss
  Model: alpha Plan-Apochromat 100x/1.46 Oil DIC (UV) VIS-IR
  Magnification / NA: 100x/1.46
  Immersion: Oil
  Correction: Plan Apochromat
  Working distance: 0.11 mm
  Product code: 420792-9800
  Installed: Yes
  Specialties: tirf
  Notes: Local OpenIRIS lists 420792-9800 as 100x/1.45 Oil DIC (UV) VIS-IR. The matching ZEISS 420792-9800-000 UV-VIS-IR variant specifies NA 1.46 and a structured Free Working Distance of 0.11 mm. The same ZEISS page describes FWD as 0.10 mm in prose; the explicit specification-table value is used here.

Stages and focus drives

• Motorized XY Stage — Zeiss Motorized XY Stage
  Zeiss
  Type: Motorized XY Stage

Autofocus and triggering

• Infrared Reflection Autofocus
  Installed: Yes
  Type: Infrared Reflection Autofocus

Optical-path elements

• Filter Turret
  Cube
  Element type: Turret
  Pos 1: Filter set 38 HE (GFP) (38 HE) — emission 525/50 nm — Excitation: 470/40 nm.
  Pos 2: Filter set 43 HE (DsRed) (43 HE) — emission 605/70 nm — Excitation: 550/25 nm.
  Pos 3: Filter set 76 HE (CFP/GFP/DsRed) (76 HE) — emission 459/25 nm, 525/30 nm, 608/30 nm — Excitation: TBP 406+489+561 nm. Emission: TBP 459+525+608 nm.
  Pos 4: Filter set 77 HE (GFP/mRFP/Alexa 633) (77 HE) — emission 526/25 nm, 601/30 nm, 688/40 nm — Excitation: TBP 483+564+642 nm. Emission: TBP 526+601+688 nm.

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Latest QC measurements

Laser performance

No recent laser-performance measurements are recorded.

Optical resolution (PSF and alignment)

No recent PSF or chromatic-shift measurements are recorded for these objectives.

Objectives without recent measurements

• EC Epiplan-Neofluar 2.5x/0.06 AIR (2.5x_air)

• EC Plan-Neofluar 10x/0.3 AIR (10x_air)

• Plan-Apochromat 20x/0.8 AIR (20x_air)

• LD Achroplan 40x/0.60 Korr Ph2 AIR (40x_air)

• alpha Plan-Apochromat 63x/1.46 Oil Corr TIRF OIL (63x_oil)

• alpha Plan-Apochromat 100x/1.46 Oil DIC (UV) VIS-IR OIL (100x_oil)

Information still to confirm

Some details in the facility record are incomplete. If any of these matter for your experiment, please check them with AIC Turku staff.

• Details needed for this configuration:

• Software version (software[].version)