Deltavision OMX
Work in progress
This dashboard is a work in progress. Please independently verify critical information, as not all features are working yet.
Current Status: ๐ก Warning
Limited to TIRF imaging only
๐ View QC & Maintenance History ๐ฌ Open Virtual Microscope
Software Environment
- OMX Acquisition (v3.70)
Acquisition
Role: Acquisition
Version:3.70
Notes: Master Control on Windows. Image processing on CentOS with softWoRx 6.0 or newer for deconvolution/reconstruction.
Hardware Configuration
๐ก๏ธ Environmental Control
- Environmental Control
controlled temperature, controlled CO2, controlled humidity
Temperature control: Yes
CO2 control: Yes
Humidity control: Yes
Notes: Onstage incubator/heating assembly. Includes insulated sample holder, heating block, objective lens heater, and gas delivery system.
๐ก Light Sources
-
405 nm Laser GE Healthcare
GE Healthcare
Type: Laser
Role: Excitation
Technology: diode
Wavelength:405 nm
Power:100 mW
Notes: Ultra-high speed Galvanometer shutter (0.2 ms). -
445 nm Laser GE Healthcare
GE Healthcare
Type: Laser
Role: Excitation
Technology: diode
Wavelength:445 nm
Power:100 mW
Notes: Ultra-high speed Galvanometer shutter (0.2 ms). -
488 nm Laser GE Healthcare
GE Healthcare
Type: Laser
Role: Excitation
Technology: dpss
Wavelength:488 nm
Power:100 mW
Notes: Ultra-high speed Galvanometer shutter (0.2 ms). -
514 nm Laser GE Healthcare
GE Healthcare
Type: Laser
Role: Excitation
Technology: dpss
Wavelength:514 nm
Power:100 mW
Notes: Ultra-high speed Galvanometer shutter (0.2 ms). -
568 nm Laser GE Healthcare
GE Healthcare
Type: Laser
Role: Excitation
Technology: dpss
Wavelength:568 nm
Power:100 mW
Notes: Ultra-high speed Galvanometer shutter (0.2 ms). -
642 nm Laser GE Healthcare
GE Healthcare
Type: Laser
Role: Excitation
Technology: diode
Wavelength:642 nm
Power:110 mW
Notes: Ultra-high speed Galvanometer shutter (0.2 ms).
๐ท Detectors
-
PCO Edge
Scientific CMOS
Type: Scientific CMOS
Pixel pitch:6.5 ยตm
Sensor format:1024 x 1024
QE peak:60%
Notes: Liquid cooled sCMOS cameras. 15 bit, 5.5 Megapixel chip. Operation modes include 3D-SIM (512 x 512 after SIM reconstruction) and Widefield/TIRF. Cam1 DAPI+Cy5/CFP. -
PCO Edge
Scientific CMOS
Type: Scientific CMOS
Pixel pitch:6.5 ยตm
Sensor format:1024 x 1024
QE peak:60%
Notes: Liquid cooled sCMOS cameras. 15 bit, 5.5 Megapixel chip. Operation modes include 3D-SIM (512 x 512 after SIM reconstruction) and Widefield/TIRF. Cam2 Alexa 488/YFP -
PCO Edge
Scientific CMOS
Type: Scientific CMOS
Pixel pitch:6.5 ยตm
Sensor format:1024 x 1024
QE peak:60%
Notes: Liquid cooled sCMOS cameras. 15 bit, 5.5 Megapixel chip. Operation modes include 3D-SIM (512 x 512 after SIM reconstruction) and Widefield/TIRF. Cam3 Alexa 568.
๐ฌ Objectives
- Plan Apo N 60x/1.42 OILAsk Staff to Install
Olympus
Model: Plan Apo N
Magnification / NA:60x/1.42
Immersion: Oil
Correction: Plan Apochromat
Working distance:0.15 mm
AFC compatible: No
Installed: No
Specialties: sim
- APO N TIRF 60x/1.49 OIL
Olympus
Model: APO N TIRF
Magnification / NA:60x/1.49
Immersion: Oil
Correction: Apochromat
Working distance:0.10 mm
AFC compatible: No
Installed: Yes
Specialties: tirf
Notes: Equipped with correction collar.
๐งญ Stages & Focus Drives
- Z Piezo Stage โ GE Healthcare Nanomotion Stage with Piezo Z-axis
GE Healthcare
Type: Z Piezo Stage
โ๏ธ Advanced Hardware
- Infrared Reflection Autofocus
Installed: Yes
Type: Infrared Reflection Autofocus
๐๏ธ Optical Path Elements
- OMX Emission Filters
Emission
Element type: Filter Wheel
Pos 1: DAPI โ emission 436/31 nm
Pos 2: CFP โ emission 478/35 nm
Pos 3: Alexa 488 โ emission 528/45 nm
Pos 4: YFP โ emission 541/22 nm
Pos 5: Alexa 568 โ emission 609/37 nm
Pos 6: Cy5 โ emission 683/40 nm
QC Metrics: Latest Measurements
๐ฆ Laser Performance
No laser performance metrics recorded recently.
๐ฌ Optical Resolution (PSF & Alignment)
No recent PSF or chromatic shift metrics available for any objectives.
Objectives without recent data
-
Plan Apo N 60x/1.42 OIL (
60x_oil) -
APO N TIRF 60x/1.49 OIL (
60x_oil_2)
Metadata Completeness
-
Legacy aliases in use (fallback accepted):
-
modules[].nameโmodules[].type -
hardware.detectors[].pixel_size_umโhardware.detectors[].pixel_pitch_um -
hardware.detectors[].min_nmโhardware.detectors[].collection_min_nm -
hardware.detectors[].max_nmโhardware.detectors[].collection_max_nm -
hardware.detectors[].channel_center_nmโhardware.detectors[].collection_center_nm -
hardware.detectors[].bandwidth_nmโhardware.detectors[].collection_width_nm