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Deltavision OMX

Work in progress

This dashboard is a work in progress. Please independently verify critical information, as not all features are working yet.

Current Status: ๐ŸŸก Warning

Limited to TIRF imaging only

๐Ÿ“Š View QC & Maintenance History ๐Ÿ”ฌ Open Virtual Microscope

Deltavision OMX
Manufacturer
GE Healthcare
Model
OMX V4
Stand
Inverted
Eyepieces
Camera-only
Location
BioCity, 5th floor, room 5149
Purchased
2016
Funding
โ€”
Modalities
Widefield Fluorescence Total Internal Reflection Fluorescence Structured Illumination Microscopy Single-Molecule Localization Microscopy
Modules
Environmental Enclosure Hardware Autofocus Piezo Z-Stage 3D-SIM Module TIRF Module


Software Environment

  • OMX Acquisition (v3.70)
    Acquisition
    Role: Acquisition
    Version: 3.70
    Notes: Master Control on Windows. Image processing on CentOS with softWoRx 6.0 or newer for deconvolution/reconstruction.

Hardware Configuration

๐ŸŒก๏ธ Environmental Control

  • Environmental Control
    controlled temperature, controlled CO2, controlled humidity
    Temperature control: Yes
    CO2 control: Yes
    Humidity control: Yes
    Notes: Onstage incubator/heating assembly. Includes insulated sample holder, heating block, objective lens heater, and gas delivery system.

๐Ÿ’ก Light Sources

  • 405 nm Laser GE Healthcare
    GE Healthcare
    Type: Laser
    Role: Excitation
    Technology: diode
    Wavelength: 405 nm
    Power: 100 mW
    Notes: Ultra-high speed Galvanometer shutter (0.2 ms).

  • 445 nm Laser GE Healthcare
    GE Healthcare
    Type: Laser
    Role: Excitation
    Technology: diode
    Wavelength: 445 nm
    Power: 100 mW
    Notes: Ultra-high speed Galvanometer shutter (0.2 ms).

  • 488 nm Laser GE Healthcare
    GE Healthcare
    Type: Laser
    Role: Excitation
    Technology: dpss
    Wavelength: 488 nm
    Power: 100 mW
    Notes: Ultra-high speed Galvanometer shutter (0.2 ms).

  • 514 nm Laser GE Healthcare
    GE Healthcare
    Type: Laser
    Role: Excitation
    Technology: dpss
    Wavelength: 514 nm
    Power: 100 mW
    Notes: Ultra-high speed Galvanometer shutter (0.2 ms).

  • 568 nm Laser GE Healthcare
    GE Healthcare
    Type: Laser
    Role: Excitation
    Technology: dpss
    Wavelength: 568 nm
    Power: 100 mW
    Notes: Ultra-high speed Galvanometer shutter (0.2 ms).

  • 642 nm Laser GE Healthcare
    GE Healthcare
    Type: Laser
    Role: Excitation
    Technology: diode
    Wavelength: 642 nm
    Power: 110 mW
    Notes: Ultra-high speed Galvanometer shutter (0.2 ms).

๐Ÿ“ท Detectors

  • PCO Edge
    Scientific CMOS
    Type: Scientific CMOS
    Pixel pitch: 6.5 ยตm
    Sensor format: 1024 x 1024
    QE peak: 60%
    Notes: Liquid cooled sCMOS cameras. 15 bit, 5.5 Megapixel chip. Operation modes include 3D-SIM (512 x 512 after SIM reconstruction) and Widefield/TIRF. Cam1 DAPI+Cy5/CFP.

  • PCO Edge
    Scientific CMOS
    Type: Scientific CMOS
    Pixel pitch: 6.5 ยตm
    Sensor format: 1024 x 1024
    QE peak: 60%
    Notes: Liquid cooled sCMOS cameras. 15 bit, 5.5 Megapixel chip. Operation modes include 3D-SIM (512 x 512 after SIM reconstruction) and Widefield/TIRF. Cam2 Alexa 488/YFP

  • PCO Edge
    Scientific CMOS
    Type: Scientific CMOS
    Pixel pitch: 6.5 ยตm
    Sensor format: 1024 x 1024
    QE peak: 60%
    Notes: Liquid cooled sCMOS cameras. 15 bit, 5.5 Megapixel chip. Operation modes include 3D-SIM (512 x 512 after SIM reconstruction) and Widefield/TIRF. Cam3 Alexa 568.

๐Ÿ”ฌ Objectives

  • Plan Apo N 60x/1.42 OILAsk Staff to Install
    Olympus
    Model: Plan Apo N
    Magnification / NA: 60x/1.42
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.15 mm
    AFC compatible: No
    Installed: No
    Specialties: sim
  • APO N TIRF 60x/1.49 OIL
    Olympus
    Model: APO N TIRF
    Magnification / NA: 60x/1.49
    Immersion: Oil
    Correction: Apochromat
    Working distance: 0.10 mm
    AFC compatible: No
    Installed: Yes
    Specialties: tirf
    Notes: Equipped with correction collar.

๐Ÿงญ Stages & Focus Drives

  • Z Piezo Stage โ€” GE Healthcare Nanomotion Stage with Piezo Z-axis
    GE Healthcare
    Type: Z Piezo Stage

โš™๏ธ Advanced Hardware

  • Infrared Reflection Autofocus
    Installed: Yes
    Type: Infrared Reflection Autofocus

๐ŸŽ›๏ธ Optical Path Elements

  • OMX Emission Filters
    Emission
    Element type: Filter Wheel
    Pos 1: DAPI โ€” emission 436/31 nm
    Pos 2: CFP โ€” emission 478/35 nm
    Pos 3: Alexa 488 โ€” emission 528/45 nm
    Pos 4: YFP โ€” emission 541/22 nm
    Pos 5: Alexa 568 โ€” emission 609/37 nm
    Pos 6: Cy5 โ€” emission 683/40 nm

QC Metrics: Latest Measurements

๐Ÿ”ฆ Laser Performance

No laser performance metrics recorded recently.

๐Ÿ”ฌ Optical Resolution (PSF & Alignment)

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data
  • Plan Apo N 60x/1.42 OIL (60x_oil)

  • APO N TIRF 60x/1.49 OIL (60x_oil_2)

Metadata Completeness

  • Legacy aliases in use (fallback accepted):

  • modules[].name โ†’ modules[].type

  • hardware.detectors[].pixel_size_um โ†’ hardware.detectors[].pixel_pitch_um

  • hardware.detectors[].min_nm โ†’ hardware.detectors[].collection_min_nm

  • hardware.detectors[].max_nm โ†’ hardware.detectors[].collection_max_nm

  • hardware.detectors[].channel_center_nm โ†’ hardware.detectors[].collection_center_nm

  • hardware.detectors[].bandwidth_nm โ†’ hardware.detectors[].collection_width_nm