Deltavision OMX

About this microscope

Here you’ll find the hardware and capabilities currently recorded for this microscope. If you are planning an experiment, AIC Turku staff can help with access, training, and configuration choices.

Current status: 🔴 Offline

Out of service. Only 405 nm and 647 nm TIRF are reported working; the system is not available for general use.

QC and maintenance history Open Virtual Microscope

Manufacturer

GE Healthcare

Model

OMX V4

Stand

Inverted

Eyepieces

Camera-only

Location

BioCity, 5th floor, room 5149

Purchased

2016

Funding

—

Capabilities

These are the capabilities recorded for this microscope. Open the optical-route details below to see how the hardware is connected.

Widefield fluorescenceTIRFSIMSMLMLive cell imagingSuper-resolutionSingle-molecule imaging

Methods and the light path that records them

Each method below is explicitly associated in the instrument record with the physical light path that implements it. An imaging mode or contrast method that appears above but not here has no recorded method-to-path mapping yet.

• Widefield fluorescence: Widefield fluorescence, TIRF, SIM, SMLM

Modules

Environmental Enclosure Hardware Autofocus Piezo Z-Stage 3D-SIM Module TIRF Module

Software

• OMX Acquisition (v3.70)
  Acquisition
  Role: Acquisition
  Version: 3.70
  Notes: Master Control on Windows. Image processing on CentOS with softWoRx 6.0 or newer for deconvolution/reconstruction.

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Hardware

Environmental control

• Environmental Control
  controlled temperature, controlled CO2, controlled humidity
  Temperature control: Yes
  CO2 control: Yes
  Humidity control: Yes
  Notes: Onstage incubator/heating assembly. Includes insulated sample holder, heating block, objective lens heater, and gas delivery system.

Light sources

• 405 nm Laser GE Healthcare
  GE Healthcare
  Type: Laser
  Role: Excitation
  Technology: diode
  Wavelength: 405 nm
  Power: 100 mW
  Notes: Ultra-high speed Galvanometer shutter (0.2 ms).

• 445 nm Laser GE Healthcare
  GE Healthcare
  Type: Laser
  Role: Excitation
  Technology: diode
  Wavelength: 445 nm
  Power: 100 mW
  Notes: Ultra-high speed Galvanometer shutter (0.2 ms).

• 488 nm Laser GE Healthcare
  GE Healthcare
  Type: Laser
  Role: Excitation
  Technology: dpss
  Wavelength: 488 nm
  Power: 100 mW
  Notes: Ultra-high speed Galvanometer shutter (0.2 ms).

• 514 nm Laser GE Healthcare
  GE Healthcare
  Type: Laser
  Role: Excitation
  Technology: dpss
  Wavelength: 514 nm
  Power: 100 mW
  Notes: Ultra-high speed Galvanometer shutter (0.2 ms).

• 568 nm Laser GE Healthcare
  GE Healthcare
  Type: Laser
  Role: Excitation
  Technology: dpss
  Wavelength: 568 nm
  Power: 100 mW
  Notes: Ultra-high speed Galvanometer shutter (0.2 ms).

• 642 nm Laser GE Healthcare
  GE Healthcare
  Type: Laser
  Role: Excitation
  Technology: diode
  Wavelength: 642 nm
  Power: 110 mW
  Notes: Ultra-high speed Galvanometer shutter (0.2 ms).

Detectors

• PCO Edge
  Scientific CMOS
  Type: Scientific CMOS
  Pixel pitch: 6.5 µm
  Sensor format: 1024 x 1024
  QE peak: 60%
  Notes: Liquid cooled sCMOS cameras. 15 bit, 5.5 Megapixel chip. Operation modes include 3D-SIM (512 x 512 after SIM reconstruction) and Widefield/TIRF. Cam1 DAPI+Cy5/CFP.

• PCO Edge
  Scientific CMOS
  Type: Scientific CMOS
  Pixel pitch: 6.5 µm
  Sensor format: 1024 x 1024
  QE peak: 60%
  Notes: Liquid cooled sCMOS cameras. 15 bit, 5.5 Megapixel chip. Operation modes include 3D-SIM (512 x 512 after SIM reconstruction) and Widefield/TIRF. Cam2 Alexa 488/YFP

• PCO Edge
  Scientific CMOS
  Type: Scientific CMOS
  Pixel pitch: 6.5 µm
  Sensor format: 1024 x 1024
  QE peak: 60%
  Notes: Liquid cooled sCMOS cameras. 15 bit, 5.5 Megapixel chip. Operation modes include 3D-SIM (512 x 512 after SIM reconstruction) and Widefield/TIRF. Cam3 Alexa 568.

Objectives

View this microscope's objectives in the catalogue

• Plan Apo N 60x/1.42 OILNot installed
  Olympus
  Model: Plan Apo N
  Magnification / NA: 60x/1.42
  Immersion: Oil
  Correction: Plan Apochromat
  Working distance: 0.15 mm
  AFC compatible: No
  Installed: No
  Specialties: sim

• APO N TIRF 60x/1.49 OIL
  Olympus
  Model: APO N TIRF
  Magnification / NA: 60x/1.49
  Immersion: Oil
  Correction: Apochromat
  Working distance: 0.10 mm
  AFC compatible: No
  Installed: Yes
  Specialties: tirf
  Notes: Equipped with correction collar.

Stages and focus drives

• Z Piezo Stage — GE Healthcare Nanomotion Stage with Piezo Z-axis
  GE Healthcare
  Type: Z Piezo Stage

Autofocus and triggering

• Infrared Reflection Autofocus
  Installed: Yes
  Type: Infrared Reflection Autofocus

Optical-path elements

• OMX Emission Filters
  Emission
  Element type: Filter Wheel
  Pos 1: DAPI — emission 436/31 nm
  Pos 2: CFP — emission 478/35 nm
  Pos 3: Alexa 488 — emission 528/45 nm
  Pos 4: YFP — emission 541/22 nm
  Pos 5: Alexa 568 — emission 609/37 nm
  Pos 6: Cy5 — emission 683/40 nm

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Latest QC measurements

Laser performance

No recent laser-performance measurements are recorded.

Optical resolution (PSF and alignment)

No recent PSF or chromatic-shift measurements are recorded for these objectives.

Objectives without recent measurements

• Plan Apo N 60x/1.42 OIL (60x_oil)

• APO N TIRF 60x/1.49 OIL (60x_oil_2)