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Leica DM RB

About this microscope

Here you’ll find the hardware and capabilities currently recorded for this microscope. If you are planning an experiment, AIC Turku staff can help with access, training, and configuration choices.

Current status: 🟢 Online

Operational

QC and maintenance history Open Virtual Microscope

Leica DM RB microscope
Manufacturer
Leica Microsystems
Model
Leica DM RB
Stand
Upright
Eyepieces
Trinocular
Location
BioCity, 2nd floor, room 2076
Purchased
—
Funding
—
Capabilities
These are the capabilities recorded for this microscope. Open the optical-route details below to see how the hardware is connected.
Widefield fluorescenceTransmitted brightfieldDarkfield
Methods and the light path that records them
Each method below is explicitly associated in the instrument record with the physical light path that implements it. An imaging mode or contrast method that appears above but not here has no recorded method-to-path mapping yet.
  • Widefield fluorescence: Widefield fluorescence
  • Transmitted light: Transmitted brightfield, Darkfield


Software

No acquisition/control software — manual or standalone system.

Hardware

Light sources

  • Halogen Lamp Leica 12V 100W halogen bulb
    Leica
    Type: Halogen Lamp
    Role: Transmitted Illumination
    Power: 100 W
    Notes: Transmitted light source for Brightfield and Darkfield. Broadband illumination.

  • Arc Lamp Osram 50W HBO short arc bulb
    Osram
    Type: Arc Lamp
    Power: 50 W
    Notes: Fluorescence excitation source. Broadband illumination.

Detectors

  • Unknown Unknown Camera
    Charge-Coupled Device
    Type: Charge-Coupled Device
    Notes: Camera port for optional detector.

Objectives

View this microscope's objectives in the catalogue

  • PL FLUOTAR 10x/0.30 AIR
    Leica
    Model: PL FLUOTAR 10x/0.30
    Magnification / NA: 10x/0.3
    Immersion: Air
    Correction: Plan Fluorite
    Product code: 506000
    AFC compatible: No
    Installed: Yes

  • HC PL APO 20x/0.70 CS AIR
    Leica
    Model: HC PL APO 20x/0.70 CS
    Magnification / NA: 20x/0.7
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 0.59 mm
    Product code: 506513
    AFC compatible: No
    Installed: Yes
    Notes: Working distance cross-checked against Leica HCS legacy objective documentation for product 506513.

  • PL FLUOTAR 40x/1.00-0.50 OIL OIL
    Leica
    Model: PL FLUOTAR 40x/1.00-0.50 OIL
    Magnification / NA: 40x/1
    Immersion: Oil
    Correction: Plan Fluorite
    Working distance: 0.08 mm
    Product code: 506007
    AFC compatible: No
    Installed: Yes
    Notes: NA range 1.00-0.50. Note that this has an adjustable iris diaphragm. Working distance matches the exact 506007 record in the AIC objective-pool source.

  • HCX PL APO 63x/1.32-0.6 OIL OIL
    Leica
    Model: HCX PL APO 63x/1.32-0.6 OIL
    Magnification / NA: 63x/1.32
    Immersion: Oil
    Correction: Plan Apochromat
    Product code: 506180
    AFC compatible: No
    Installed: Yes
    Notes: NA range 1.32-0.6. Has an adjustable iris diaphragm for darkfield.

  • PL APO 100x/1.40-0.7 OIL OIL
    Leica
    Model: PL APO 100x/1.40-0.7 OIL
    Magnification / NA: 100x/1.4
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.09 mm
    Product code: 506038
    AFC compatible: No
    Installed: Yes
    Notes: NA range 1.40-0.7. Has an adjustable iris diaphragm for darkfield.

Optical-path elements

  • Fluorescence Turret
    Cube
    Element type: Turret
    Pos 1: Filter Cube A (DAPI/Hoechst) — Excitation: 360/40 nm. Dichroic Cutoff: 400 nm.
    Pos 2: Filter Cube EGFP — emission 525/50 nm — Excitation: 470/40 nm. Dichroic Cutoff: 495 nm.
    Pos 3: Filter Cube CY3 (TRITC/RFP) — emission 605/70 nm — Excitation: 545.5/25 nm. Dichroic Cutoff: 565 nm.

Latest QC measurements

Laser performance

No recent laser-performance measurements are recorded.

Optical resolution (PSF and alignment)

No recent PSF or chromatic-shift measurements are recorded for these objectives.

Objectives without recent measurements

  • PL FLUOTAR 10x/0.30 AIR (10x_air)

  • HC PL APO 20x/0.70 CS AIR (20x_air)

  • PL FLUOTAR 40x/1.00-0.50 OIL OIL (40x_oil)

  • HCX PL APO 63x/1.32-0.6 OIL OIL (63x_oil)

  • PL APO 100x/1.40-0.7 OIL OIL (100x_oil)

Information still to confirm

Some details in the facility record are incomplete. If any of these matter for your experiment, please check them with AIC Turku staff.

  • Details needed for this configuration:

  • Detector pixel pitch (um) (hardware.detectors[].pixel_pitch_um)