Leica DM IRBE

About this microscope

Here you’ll find the hardware and capabilities currently recorded for this microscope. If you are planning an experiment, AIC Turku staff can help with access, training, and configuration choices.

Current status: 🟢 Online

Operational

QC and maintenance history Open Virtual Microscope

Manufacturer

Leica Microsystems

Model

Leica DM IRBE

Stand

Inverted

Eyepieces

Trinocular

Location

BioCity, 2nd floor, room 2076

Purchased

—

Funding

—

Capabilities

These are the capabilities recorded for this microscope. Open the optical-route details below to see how the hardware is connected.

Widefield fluorescenceTransmitted brightfieldPhase contrastDarkfield

Methods and the light path that records them

Each method below is explicitly associated in the instrument record with the physical light path that implements it. An imaging mode or contrast method that appears above but not here has no recorded method-to-path mapping yet.

• Widefield fluorescence: Widefield fluorescence
• Transmitted light: Transmitted brightfield, Phase contrast, Darkfield

Software

No acquisition/control software — manual or standalone system.

---

Hardware

Light sources

• Halogen Lamp Leica 12V 100W halogen bulb
  Leica
  Type: Halogen Lamp
  Role: Transmitted Illumination
  Power: 100 W
  Notes: Transmitted light source for BF, DF, and PH. Broadband illumination.

• Arc Lamp Leica 50W HBO short arc bulb
  Leica
  Type: Arc Lamp
  Power: 50 W
  Notes: Fluorescence excitation source. Broadband illumination.

Detectors

• Unknown Unknown Camera
  Charge-Coupled Device
  Type: Charge-Coupled Device
  Notes: Standalone camera system attached to port (no computer required); image storage via USB memory device.

Objectives

View this microscope's objectives in the catalogue

• PL FLUOTAR 10x/0.30 PH1 AIR
  Leica
  Model: PL FLUOTAR 10x/0.30 PH1
  Magnification / NA: 10x/0.3
  Immersion: Air
  Correction: Plan Fluorite
  Working distance: 3.8 mm
  Product code: 506010
  AFC compatible: No
  Installed: Yes
  Specialties: phase
  Notes: Working distance verified against an exact 506010 legacy objective listing.

• PL FLUOTAR 20x/0.50 PH2 AIR
  Leica
  Model: PL FLUOTAR 20x/0.50 PH2
  Magnification / NA: 20x/0.5
  Immersion: Air
  Correction: Plan Fluorite
  Product code: 506013
  AFC compatible: No
  Installed: Yes
  Specialties: phase

• PL FLUOTAR 40x/0.70 PH2 AIR
  Leica
  Model: PL FLUOTAR 40x/0.70 PH2
  Magnification / NA: 40x/0.7
  Immersion: Air
  Correction: Plan Fluorite
  Product code: 506014
  AFC compatible: No
  Installed: Yes
  Specialties: phase

• PL FLUOTAR 63x/0.70 CORR PH2 AIR
  Leica
  Model: PL FLUOTAR 63x/0.70 CORR PH2
  Magnification / NA: 63x/0.7
  Immersion: Air
  Correction: Plan Fluorite
  Working distance: 2.6-1.8 mm
  Product code: 506062
  AFC compatible: No
  Installed: Yes
  Specialties: phase, correction_collar
  Notes: Working-distance range cross-checked against an exact 506062 legacy objective listing.

Magnification changers

• 1.6x Auxiliary Lens
  Leica
  Manufacturer: Leica
  Model: 1.6x Auxiliary Lens
  Magnification: 1.6x
  Notes: Optional 1.6x auxiliary lens in microscope body.

Optical-path elements

• Fluorescence Turret
  Cube
  Element type: Turret
  Pos 1: Filter Cube A (DAPI/Hoechst) — Excitation: 360/40 nm. Dichroic Cutoff: 400 nm.
  Pos 2: Filter Cube EGFP — emission 525/50 nm — Excitation: 470/40 nm. Dichroic Cutoff: 495 nm.
  Pos 3: Filter Cube CY3 (TRITC/RFP) — emission 605/70 nm — Excitation: 545.5/25 nm. Dichroic Cutoff: 565 nm.

---

Latest QC measurements

Laser performance

No recent laser-performance measurements are recorded.

Optical resolution (PSF and alignment)

No recent PSF or chromatic-shift measurements are recorded for these objectives.

Objectives without recent measurements

• PL FLUOTAR 10x/0.30 PH1 AIR (10x_air)

• PL FLUOTAR 20x/0.50 PH2 AIR (20x_air)

• PL FLUOTAR 40x/0.70 PH2 AIR (40x_air)

• PL FLUOTAR 63x/0.70 CORR PH2 AIR (63x_air)

Information still to confirm

Some details in the facility record are incomplete. If any of these matter for your experiment, please check them with AIC Turku staff.

• Details needed for this configuration:

• Detector pixel pitch (um) (hardware.detectors[].pixel_pitch_um)