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Agilent xCELLigence RTCA eSight

Work in progress

This dashboard is a work in progress. Please independently verify critical information, as not all features are working yet.

Current Status: 🟢 Online

Operational

📊 View QC & Maintenance History 🔬 Open Virtual Microscope

Agilent xCELLigence RTCA eSight
Manufacturer
Agilent
Model
xCELLigence RTCA eSight
Stand
Benchtop
Eyepieces
Camera-only
Location
BioCity, 5th floor
Purchased
2024
Funding
Testament funds from Henna Ruusunen
Modalities
Impedance Cytometry Widefield Fluorescence Transmitted Brightfield Live Cell Imaging
Modules
Environmental Enclosure Hardware Autofocus Impedance Module


Software Environment

Hardware Configuration

🌡️ Environmental Control

  • Environmental Control
    controlled temperature, controlled CO2, controlled humidity
    Temperature control: Yes
    CO2 control: Yes
    Humidity control: Yes
    Notes: Operates inside a standard cell culture incubator.

💡 Light Sources

  • 393 nm LED Agilent High-power LED (Blue)
    Agilent
    Type: LED
    Wavelength: 393 nm
    Notes: Blue Excitation (381-404 nm)

  • 482 nm LED Agilent High-power LED (Green)
    Agilent
    Type: LED
    Wavelength: 482 nm
    Notes: Green Excitation (473-491 nm)

  • 595 nm LED Agilent High-power LED (Red)
    Agilent
    Type: LED
    Wavelength: 595 nm
    Notes: Red Excitation (584-605 nm)

  • LED Agilent Transmitted LED
    Agilent
    Type: LED
    Role: Transmitted Illumination
    Notes: For brightfield imaging

📷 Detectors

  • Sony 5.0 MP Monochromatic CMOS
    Standard CMOS
    Type: Standard CMOS
    Pixel pitch: 3.45 µm
    Notes: up to 35 fps.

🔬 Objectives

  • 10x Objective 10x/0.3 AIR
    Agilent
    Model: 10x Objective
    Magnification / NA: 10x/0.3
    Immersion: Air
    Correction: Plan Achromat
    AFC compatible: Yes
    Installed: Yes
    Notes: Default. Resolution: 1.72 µm/pixel. FOV: 1.68 x 1.40 mm.

⚙️ Advanced Hardware

  • Contrast-Based Autofocus
    Installed: Yes
    Type: Contrast-Based Autofocus

🎛️ Optical Path Elements

  • Internal Filter Turret
    Cube
    Element type: Turret
    Pos 1: Blue Channel — emission 445/35 nm — e.g., DAPI/Hoechst. Excitation: 381-404 nm.
    Pos 2: Green Channel — emission 532/58 nm — e.g., FITC/GFP. Excitation: 473-491 nm.
    Pos 3: Red Channel — emission 671/99 nm — e.g., Cy3/Texas Red. Excitation: 584-605 nm.

QC Metrics: Latest Measurements

🔦 Laser Performance

No laser performance metrics recorded recently.

🔬 Optical Resolution (PSF & Alignment)

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data
  • 10x Objective 10x/0.3 AIR (10x_air)

Metadata Completeness

  • Missing conditional fields (currently applicable):

  • software[].version — Software version

  • Legacy aliases in use (fallback accepted):

  • modules[].name → modules[].type

  • hardware.detectors[].pixel_size_um → hardware.detectors[].pixel_pitch_um

  • hardware.detectors[].min_nm → hardware.detectors[].collection_min_nm

  • hardware.detectors[].max_nm → hardware.detectors[].collection_max_nm

  • hardware.detectors[].channel_center_nm → hardware.detectors[].collection_center_nm

  • hardware.detectors[].bandwidth_nm → hardware.detectors[].collection_width_nm