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Agilent xCELLigence RTCA eSight

About this microscope

Here you’ll find the hardware and capabilities currently recorded for this microscope. If you are planning an experiment, AIC Turku staff can help with access, training, and configuration choices.

Current status: 🟢 Online

Operational

QC and maintenance history Open Virtual Microscope

Agilent xCELLigence RTCA eSight microscope
Manufacturer
Agilent
Model
xCELLigence RTCA eSight
Stand
Benchtop
Eyepieces
Camera-only
Location
BioCity, 5th floor
Purchased
2024
Funding
Testament funds from Henna Ruusunen
Capabilities
These are the capabilities recorded for this microscope. Open the optical-route details below to see how the hardware is connected.
Widefield fluorescenceTransmitted brightfieldLive cell imagingImpedance cytometry
Methods and the light path that records them
Each method below is explicitly associated in the instrument record with the physical light path that implements it. An imaging mode or contrast method that appears above but not here has no recorded method-to-path mapping yet.
  • Widefield fluorescence: Widefield fluorescence
  • Transmitted light: Transmitted brightfield
Modules
Environmental Enclosure Hardware Autofocus Impedance Module


Software

Hardware

Environmental control

  • Environmental Control
    controlled temperature, controlled CO2, controlled humidity
    Temperature control: Yes
    CO2 control: Yes
    Humidity control: Yes
    Notes: Operates inside a standard cell culture incubator.

Light sources

  • 393 nm LED Agilent High-power LED (Blue)
    Agilent
    Type: LED
    Wavelength: 393 nm
    Notes: Blue Excitation (381-404 nm)

  • 482 nm LED Agilent High-power LED (Green)
    Agilent
    Type: LED
    Wavelength: 482 nm
    Notes: Green Excitation (473-491 nm)

  • 595 nm LED Agilent High-power LED (Red)
    Agilent
    Type: LED
    Wavelength: 595 nm
    Notes: Red Excitation (584-605 nm)

  • LED Agilent Transmitted LED
    Agilent
    Type: LED
    Role: Transmitted Illumination
    Notes: For brightfield imaging

Detectors

  • Sony 5.0 MP Monochromatic CMOS
    Standard CMOS
    Type: Standard CMOS
    Pixel pitch: 3.45 µm
    Notes: up to 35 fps.

Objectives

View this microscope's objectives in the catalogue

  • 10x Objective 10x/0.3 AIR
    Agilent
    Model: 10x Objective
    Magnification / NA: 10x/0.3
    Immersion: Air
    Correction: Plan Achromat
    AFC compatible: Yes
    Installed: Yes
    Notes: Default. Resolution: 1.72 µm/pixel. FOV: 1.68 x 1.40 mm.

Autofocus and triggering

  • Contrast-Based Autofocus
    Installed: Yes
    Type: Contrast-Based Autofocus

Optical-path elements

  • Internal Filter Turret
    Cube
    Element type: Turret
    Pos 1: Blue Channel — emission 445/35 nm — e.g., DAPI/Hoechst. Excitation: 381-404 nm.
    Pos 2: Green Channel — emission 532/58 nm — e.g., FITC/GFP. Excitation: 473-491 nm.
    Pos 3: Red Channel — emission 671/99 nm — e.g., Cy3/Texas Red. Excitation: 584-605 nm.

Latest QC measurements

Laser performance

No recent laser-performance measurements are recorded.

Optical resolution (PSF and alignment)

No recent PSF or chromatic-shift measurements are recorded for these objectives.

Objectives without recent measurements
  • 10x Objective 10x/0.3 AIR (10x_air)