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Nikon Eclipse Ti2-E

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Current Status: ๐ŸŸข Online

Operational

๐Ÿ“Š View QC & Maintenance History ๐Ÿ”ฌ Open Virtual Microscope

Nikon Eclipse Ti2-E
Manufacturer
Nikon
Model
Eclipse Ti2-E
Stand
Inverted
Eyepieces
Trinocular
Location
โ€”
Purchased
2017
Funding
โ€”
Modalities
Widefield Fluorescence Transmitted Brightfield Differential Interference Contrast
Modules
Incubation Hardware Autofocus


Software Environment

  • Nikon NIS-Elements AR (v5.11)
    Acquisition
    Role: Acquisition
    Version: 5.11

Hardware Configuration

๐ŸŒก๏ธ Environmental Control

  • Environmental Control
    controlled temperature, controlled CO2, controlled humidity
    Temperature control: Yes
    CO2 control: Yes
    Humidity control: Yes
    Notes: Enclosed incubation chamber (Okolab) with temperature control (Okolab H201-T)

๐Ÿ’ก Light Sources

  • 395 nm LED Lumencor Spectra X LED system
    Lumencor
    Type: LED
    Wavelength: 395 nm

  • 440 nm LED Lumencor Spectra X LED system
    Lumencor
    Type: LED
    Wavelength: 440 nm

  • 475 nm LED Lumencor Spectra X LED system
    Lumencor
    Type: LED
    Wavelength: 475 nm

  • 550 nm LED Lumencor Spectra X LED system
    Lumencor
    Type: LED
    Wavelength: 550 nm
    Notes: 550 nm module available by request

  • 575 nm LED Lumencor Spectra X LED system
    Lumencor
    Type: LED
    Wavelength: 575 nm

  • 640 nm LED Lumencor Spectra X LED system
    Lumencor
    Type: LED
    Wavelength: 640 nm

  • 730 nm LED Lumencor Spectra X LED system
    Lumencor
    Type: LED
    Wavelength: 730 nm

  • LED Nikon Ti2 Transmitted Illuminator
    Nikon
    Type: LED
    Role: Transmitted Illumination
    Notes: Broad spectrum transmitted source for BF/DIC.

๐Ÿ“ท Detectors

  • Hamamatsu Orca Flash4.0 V3
    Scientific CMOS
    Type: Scientific CMOS
    Pixel pitch: 6.5 ยตm
    Sensor format: 2048 x 2044
    Notes: sCMOS

  • Nikon DS-Fi3
    Standard CMOS
    Type: Standard CMOS
    Pixel pitch: 2.4 ยตm
    Sensor format: 2880 x 2048
    Notes: Color CMOS

๐Ÿ”ฌ Objectives

  • Plan UW 2x/0.06 2x/0.06 AIR
    Nikon
    Model: Plan UW 2x/0.06
    Magnification / NA: 2x/0.06
    Immersion: Air
    Correction: Plan Achromat
    Working distance: 7.5 mm
    Product code: MRL00022
    AFC compatible: No
    Installed: Yes
    Specialties: polarization
    Notes: Mode suitability: BF recommended; FLUO (VIS) possible; polarization possible.

  • Plan Fluor 4x/0.13 4x/0.13 AIR
    Nikon
    Model: Plan Fluor 4x/0.13
    Magnification / NA: 4x/0.13
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 17.2 mm
    Product code: MRH00041
    AFC compatible: No
    Installed: Yes
    Specialties: polarization
    Notes: Mode suitability: BF recommended; FLUO (VIS) recommended; FLUO (UV) recommended; polarization possible.

  • Plan Apo ฮปD 4x/0.20 4x/0.2 AIR
    Nikon
    Model: Plan Apo ฮปD 4x/0.20
    Magnification / NA: 4x/0.2
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 20.0 mm
    Product code: MRD70040
    AFC compatible: No
    Installed: Yes
    Notes: Optical family: Lambda D. Mode suitability: BF recommended; FLUO (VIS) recommended; FLUO (NIR) recommended.

  • Plan Fluor 10x/0.30 OFN 25 Ph1 DL 10x/0.3 AIR
    Nikon
    Model: Plan Fluor 10x/0.30 OFN 25 Ph1 DL
    Magnification / NA: 10x/0.3
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 15.2 mm
    Product code: MRH20101
    AFC compatible: Yes
    Installed: Yes
    Specialties: phase
    Notes: OFN 25. Mode suitability: BF suitable; FLUO (VIS) suitable; FLUO (UV) suitable.

  • S Plan Fluor ELWD ADM 20x/0.45 DIC N1 20x/0.45 AIR
    Nikon
    Model: S Plan Fluor ELWD ADM 20x/0.45 DIC N1
    Magnification / NA: 20x/0.45
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 8.2 - 6.9 mm
    Product code: MRH08230
    AFC compatible: Yes
    Installed: Yes
    Specialties: long_working_distance, dic, polarization
    Notes: Mode suitability: BF recommended; FLUO (VIS) recommended; FLUO (UV) recommended; polarization suitable.

  • Plan Apo ฮป 20x/0.75 OFN25 DIC N2 20x/0.75 AIR
    Nikon
    Model: Plan Apo ฮป 20x/0.75 OFN25 DIC N2
    Magnification / NA: 20x/0.75
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 1.0 mm
    Product code: MRD00205
    AFC compatible: Yes
    Installed: Yes
    Specialties: dic, polarization
    Notes: OFN 25. Optical family: Lambda. Mode suitability: BF recommended; FLUO (VIS) recommended; FLUO (UV) recommended; polarization possible.

  • S Plan Fluor ELWD 40x/0.60 OFN22 Ph2 40x/0.6 AIR
    Nikon
    Model: S Plan Fluor ELWD 40x/0.60 OFN22 Ph2
    Magnification / NA: 40x/0.6
    Immersion: Air
    Correction: Plan Fluorite
    Working distance: 3.6 - 2.8 mm
    Product code: MRH48430
    AFC compatible: Yes
    Installed: Yes
    Specialties: long_working_distance, phase
    Notes: OFN 22. Mode suitability: BF suitable; FLUO (VIS) suitable; FLUO (UV) suitable.

  • Plan Apo ฮป 60x/1.40 Oil OFN25 DIC N2 60x/1.4 OIL
    Nikon
    Model: Plan Apo ฮป 60x/1.40 Oil OFN25 DIC N2
    Magnification / NA: 60x/1.4
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.13 mm
    Product code: MRD01605
    AFC compatible: Yes
    Installed: Yes
    Specialties: dic, polarization
    Notes: OFN 25. Optical family: Lambda. Mode suitability: BF recommended; FLUO (VIS) recommended; FLUO (NIR) recommended; polarization suitable.

  • Apo TIRF 100x/1.49 Oil 100x/1.49 OIL
    Nikon
    Model: Apo TIRF 100x/1.49 Oil
    Magnification / NA: 100x/1.49
    Immersion: Oil
    Correction: Apochromat
    Working distance: 0.16 - 0.10 mm
    Product code: MRD01991
    AFC compatible: Yes
    Installed: Yes
    Specialties: tirf, phase, dic, polarization
    Notes: Mode suitability: BF recommended; FLUO (VIS) recommended; FLUO (UV) possible; polarization suitable.

โš™๏ธ Advanced Hardware

  • Infrared Reflection Autofocus
    Installed: Yes
    Type: Infrared Reflection Autofocus

๐ŸŽ›๏ธ Optical Path Elements

  • Filter Turret
    Dichroic
    Element type: Turret
    Pos 1: Chroma 89403bs (89403bs) โ€” Dichroic for CFP/YFP/mCherry/Cy7
    Pos 2: Chroma 84000v2 (84000v2) โ€” Quad-band for DAPI/FITC/TRITC/Cy5
    Pos 3: Chroma 96377 (96377) โ€” emission 809/81 nm โ€” Cy7 emission filter housed in turret

  • Emission Wheel
    Emission
    Element type: Filter Wheel
    Pos 1: DAPI Emission โ€” emission 435/26 nm
    Pos 2: CFP Emission โ€” emission 475/20 nm
    Pos 3: GFP Emission โ€” emission 515/30 nm
    Pos 4: YFP Emission โ€” emission 540/21 nm
    Pos 5: TRITC Emission โ€” emission 595/40 nm
    Pos 6: mCherry Emission โ€” emission 632/60 nm
    Pos 7: Cy5 Emission โ€” emission 705/71 nm

  • Lamp Filter Cubes
    Cube
    Element type: Turret
    Pos 1: Standard Cubes โ€” Standard DAPI, CFP, GFP, YFP, and TexasRed cubes

  • DIC Fixed Analyzer
    Analyzer
    Element type: Slider
    Pos 1: Analyzer โ€” For DIC imaging

QC Metrics: Latest Measurements

๐Ÿ”ฆ Laser Performance

No laser performance metrics recorded recently.

๐Ÿ”ฌ Optical Resolution (PSF & Alignment)

No recent PSF or chromatic shift metrics available for any objectives.

Objectives without recent data

  • Plan UW 2x/0.06 2x/0.06 AIR (2x_air)

  • Plan Fluor 4x/0.13 4x/0.13 AIR (4x_air)

  • Plan Apo ฮปD 4x/0.20 4x/0.2 AIR (4x_air_2)

  • Plan Fluor 10x/0.30 OFN 25 Ph1 DL 10x/0.3 AIR (10x_air)

  • S Plan Fluor ELWD ADM 20x/0.45 DIC N1 20x/0.45 AIR (20x_air)

  • Plan Apo ฮป 20x/0.75 OFN25 DIC N2 20x/0.75 AIR (20x_air_2)

  • S Plan Fluor ELWD 40x/0.60 OFN22 Ph2 40x/0.6 AIR (40x_air)

  • Plan Apo ฮป 60x/1.40 Oil OFN25 DIC N2 60x/1.4 OIL (60x_oil)

  • Apo TIRF 100x/1.49 Oil 100x/1.49 OIL (100x_oil)

Metadata Completeness

  • Missing required fields:

  • instrument.location โ€” Location

  • Legacy aliases in use (fallback accepted):

  • modules[].name โ†’ modules[].type

  • hardware.detectors[].pixel_size_um โ†’ hardware.detectors[].pixel_pitch_um

  • hardware.detectors[].min_nm โ†’ hardware.detectors[].collection_min_nm

  • hardware.detectors[].max_nm โ†’ hardware.detectors[].collection_max_nm

  • hardware.detectors[].channel_center_nm โ†’ hardware.detectors[].collection_center_nm

  • hardware.detectors[].bandwidth_nm โ†’ hardware.detectors[].collection_width_nm