Nikon Eclipse Ti2-E

About this microscope

Here you’ll find the hardware and capabilities currently recorded for this microscope. If you are planning an experiment, AIC Turku staff can help with access, training, and configuration choices.

Current status: 🟢 Online

Operational

QC and maintenance history Open Virtual Microscope

Manufacturer

Nikon

Model

Eclipse Ti2-E

Stand

Inverted

Eyepieces

Trinocular

Location

BioCity, 5th floor, room Dunkel

Purchased

2017

Funding

—

Capabilities

These are the capabilities recorded for this microscope. Open the optical-route details below to see how the hardware is connected.

Widefield fluorescenceTransmitted brightfieldDICPhase contrastLive cell imagingHigh-content screening

Methods and the light path that records them

Each method below is explicitly associated in the instrument record with the physical light path that implements it. An imaging mode or contrast method that appears above but not here has no recorded method-to-path mapping yet.

Widefield fluorescence: Widefield fluorescence
Transmitted light: Transmitted brightfield, DIC, Phase contrast

Modules

Incubation Hardware Autofocus

Software

Nikon NIS-Elements AR (v6.1)
Acquisition
Role: Acquisition
Version: 6.1

Hardware

Environmental control

Environmental Control
controlled temperature, controlled CO2, controlled humidity
Temperature control: Yes
CO2 control: Yes
Humidity control: Yes
Notes: Enclosed incubation chamber (Okolab) with temperature control (Okolab H201-T)

Light sources

395 nm LED Lumencor Spectra X LED system
Lumencor
Type: LED
Wavelength: 395 nm
440 nm LED Lumencor Spectra X LED system
Lumencor
Type: LED
Wavelength: 440 nm
475 nm LED Lumencor Spectra X LED system
Lumencor
Type: LED
Wavelength: 475 nm
550 nm LED Lumencor Spectra X LED system
Lumencor
Type: LED
Wavelength: 550 nm
Notes: 550 nm module available by request
575 nm LED Lumencor Spectra X LED system
Lumencor
Type: LED
Wavelength: 575 nm
640 nm LED Lumencor Spectra X LED system
Lumencor
Type: LED
Wavelength: 640 nm
730 nm LED Lumencor Spectra X LED system
Lumencor
Type: LED
Wavelength: 730 nm
LED Nikon Ti2 Transmitted Illuminator
Nikon
Type: LED
Role: Transmitted Illumination
Notes: Broad spectrum transmitted source for BF/DIC.

Detectors

Hamamatsu Orca Flash4.0 V3
Scientific CMOS
Type: Scientific CMOS
Pixel pitch: 6.5 µm
Sensor format: 2048 x 2048
Notes: sCMOS. Native effective pixel array from the ORCA-Flash4.0 V3 manufacturer specification; not a session ROI.
Nikon DS-Fi3
Standard CMOS
Type: Standard CMOS
Pixel pitch: 2.4 µm
Sensor format: 2880 x 2048
Notes: Color CMOS

Objectives

View this microscope's objectives in the catalogue

Plan UW 2x/0.06 AIR
Nikon
Model: Plan UW 2x/0.06
Magnification / NA: 2x/0.06
Immersion: Air
Correction: Plan Achromat
Working distance: 7.5 mm
Product code: MRL00022
AFC compatible: No
Installed: Yes
Specialties: polarization
Notes: Mode suitability: BF recommended; FLUO (VIS) possible; polarization possible.
Plan Fluor 4x/0.13 AIR
Nikon
Model: Plan Fluor 4x/0.13
Magnification / NA: 4x/0.13
Immersion: Air
Correction: Plan Fluorite
Working distance: 17.2 mm
Product code: MRH00041
AFC compatible: No
Installed: Yes
Specialties: polarization
Notes: Mode suitability: BF recommended; FLUO (VIS) recommended; FLUO (UV) recommended; polarization possible.
Plan Apo λD 4x/0.20 AIR
Nikon
Model: Plan Apo λD 4x/0.20
Magnification / NA: 4x/0.2
Immersion: Air
Correction: Plan Apochromat
Working distance: 20.0 mm
Product code: MRD70040
AFC compatible: No
Installed: Yes
Notes: Optical family: Lambda D. Mode suitability: BF recommended; FLUO (VIS) recommended; FLUO (NIR) recommended.
Plan Fluor 10x/0.30 OFN 25 Ph1 DL AIR
Nikon
Model: Plan Fluor 10x/0.30 OFN 25 Ph1 DL
Magnification / NA: 10x/0.3
Immersion: Air
Correction: Plan Fluorite
Working distance: 15.2 mm
Product code: MRH20101
AFC compatible: Yes
Installed: Yes
Specialties: phase
Notes: OFN 25. Mode suitability: BF suitable; FLUO (VIS) suitable; FLUO (UV) suitable.
S Plan Fluor ELWD ADM 20x/0.45 DIC N1 AIR
Nikon
Model: S Plan Fluor ELWD ADM 20x/0.45 DIC N1
Magnification / NA: 20x/0.45
Immersion: Air
Correction: Plan Fluorite
Working distance: 8.2 - 6.9 mm
Product code: MRH08230
AFC compatible: Yes
Installed: Yes
Specialties: long_working_distance, dic, polarization
Notes: Mode suitability: BF recommended; FLUO (VIS) recommended; FLUO (UV) recommended; polarization suitable.
Plan Apo λ 20x/0.75 OFN25 DIC N2 AIR
Nikon
Model: Plan Apo λ 20x/0.75 OFN25 DIC N2
Magnification / NA: 20x/0.75
Immersion: Air
Correction: Plan Apochromat
Working distance: 1.0 mm
Product code: MRD00205
AFC compatible: Yes
Installed: Yes
Specialties: dic, polarization
Notes: OFN 25. Optical family: Lambda. Mode suitability: BF recommended; FLUO (VIS) recommended; FLUO (UV) recommended; polarization possible.
S Plan Fluor ELWD 40x/0.60 OFN22 Ph2 AIR
Nikon
Model: S Plan Fluor ELWD 40x/0.60 OFN22 Ph2
Magnification / NA: 40x/0.6
Immersion: Air
Correction: Plan Fluorite
Working distance: 3.6 - 2.8 mm
Product code: MRH48430
AFC compatible: Yes
Installed: Yes
Specialties: long_working_distance, phase
Notes: OFN 22. Mode suitability: BF suitable; FLUO (VIS) suitable; FLUO (UV) suitable.
Plan Apo λ 60x/1.40 Oil OFN25 DIC N2 OIL
Nikon
Model: Plan Apo λ 60x/1.40 Oil OFN25 DIC N2
Magnification / NA: 60x/1.4
Immersion: Oil
Correction: Plan Apochromat
Working distance: 0.13 mm
Product code: MRD01605
AFC compatible: Yes
Installed: Yes
Specialties: dic, polarization
Notes: OFN 25. Optical family: Lambda. Mode suitability: BF recommended; FLUO (VIS) recommended; FLUO (NIR) recommended; polarization suitable.
Apo TIRF 100x/1.49 Oil OIL
Nikon
Model: Apo TIRF 100x/1.49 Oil
Magnification / NA: 100x/1.49
Immersion: Oil
Correction: Apochromat
Working distance: 0.16 - 0.10 mm
Product code: MRD01991
AFC compatible: Yes
Installed: Yes
Specialties: tirf, phase, dic, polarization
Notes: Mode suitability: BF recommended; FLUO (VIS) recommended; FLUO (UV) possible; polarization suitable.

Autofocus and triggering

Infrared Reflection Autofocus
Installed: Yes
Type: Infrared Reflection Autofocus

Optical-path elements

Filter Turret
Dichroic
Element type: Turret
Pos 1: Chroma 89403bs (89403bs) — Dichroic for CFP/YFP/mCherry/Cy7
Pos 2: Chroma 84000v2 (84000v2) — Quad-band for DAPI/FITC/TRITC/Cy5
Pos 3: Chroma 96377 (96377) — emission 809/81 nm — Cy7 emission filter housed in turret
Emission Wheel
Emission
Element type: Filter Wheel
Pos 1: DAPI Emission — emission 435/26 nm
Pos 2: CFP Emission — emission 475/20 nm
Pos 3: GFP Emission — emission 515/30 nm
Pos 4: YFP Emission — emission 540/21 nm
Pos 5: TRITC Emission — emission 595/40 nm
Pos 6: mCherry Emission — emission 632/60 nm
Pos 7: Cy5 Emission — emission 705/71 nm
Lamp Filter Cubes
Cube
Element type: Turret
Pos 1: Standard Cubes — Standard DAPI, CFP, GFP, YFP, and TexasRed cubes
DIC Fixed Analyzer
Analyzer
Element type: Slider
Pos 1: Analyzer — For DIC imaging

Latest QC measurements

Laser performance

No recent laser-performance measurements are recorded.

Optical resolution (PSF and alignment)

No recent PSF or chromatic-shift measurements are recorded for these objectives.

Objectives without recent measurements

Plan UW 2x/0.06 AIR (2x_air)
Plan Fluor 4x/0.13 AIR (4x_air)
Plan Apo λD 4x/0.20 AIR (4x_air_2)
Plan Fluor 10x/0.30 OFN 25 Ph1 DL AIR (10x_air)
S Plan Fluor ELWD ADM 20x/0.45 DIC N1 AIR (20x_air)
Plan Apo λ 20x/0.75 OFN25 DIC N2 AIR (20x_air_2)
S Plan Fluor ELWD 40x/0.60 OFN22 Ph2 AIR (40x_air)
Plan Apo λ 60x/1.40 Oil OFN25 DIC N2 OIL (60x_oil)
Apo TIRF 100x/1.49 Oil OIL (100x_oil)