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Leica Thunder

About this microscope

Here you’ll find the hardware and capabilities currently recorded for this microscope. If you are planning an experiment, AIC Turku staff can help with access, training, and configuration choices.

Current status: 🟢 Online

Operational

QC and maintenance history Open Virtual Microscope

Leica Thunder microscope
Manufacturer
Leica Microsystems
Model
Leica THUNDER Imager 3D Live Cell
Stand
Inverted
Eyepieces
Trinocular
Location
BioCity, 5th floor, room Dunkel
Purchased
2023
Funding
—
Capabilities
These are the capabilities recorded for this microscope. Open the optical-route details below to see how the hardware is connected.
Widefield fluorescenceTransmitted brightfieldPhase contrastDICLive cell imaging
Methods and the light path that records them
Each method below is explicitly associated in the instrument record with the physical light path that implements it. An imaging mode or contrast method that appears above but not here has no recorded method-to-path mapping yet.
  • Widefield fluorescence: Widefield fluorescence
  • Transmitted light: Transmitted brightfield, Phase contrast, DIC
Modules
Hardware Autofocus


Software

Hardware

Environmental control

  • Environmental Control
    controlled temperature, controlled CO2, controlled humidity
    Temperature control: Yes
    CO2 control: Yes
    Humidity control: Yes

Light sources

  • 395 nm LED Leica LED 8
    Leica
    Type: LED
    Wavelength: 395 nm
    Power: 200 mW
    Notes: 390 in software

  • 438 nm LED Leica LED 8
    Leica
    Type: LED
    Wavelength: 438 nm
    Power: 200 mW
    Notes: 440 in software

  • 475 nm LED Leica LED 8
    Leica
    Type: LED
    Wavelength: 475 nm
    Power: 200 mW

  • 511 nm LED Leica LED 8
    Leica
    Type: LED
    Wavelength: 511 nm
    Power: 200 mW
    Notes: 510 in software

  • 555 nm LED Leica LED 8
    Leica
    Type: LED
    Wavelength: 555 nm
    Power: 200 mW

  • 575 nm LED Leica LED 8
    Leica
    Type: LED
    Wavelength: 575 nm
    Power: 200 mW

  • 635 nm LED Leica LED 8
    Leica
    Type: LED
    Wavelength: 635 nm
    Power: 200 mW

  • 730 nm LED Leica LED 8
    Leica
    Type: LED
    Wavelength: 730 nm
    Power: 200 mW
    Notes: 747 in software

Detectors

  • Leica K8
    Scientific CMOS
    Type: Scientific CMOS
    Pixel pitch: 6.5 µm
    Sensor format: 2048 x 2048
    Bit depth: 12
    QE peak: 95%
    Read noise: 1 e-
    Notes: monochrome; 18.8 mm sensor, dark current 1.5 e-/pix/s

  • Leica K3C
    Standard CMOS
    Type: Standard CMOS
    Pixel pitch: 3.45 µm
    Bit depth: 12
    Notes: color CMOS; excellent color reproduction; RGB quantifiable images

Objectives

View this microscope's objectives in the catalogue

  • N PLAN 5x/0.12 PH 0 AIR
    Leica
    Model: N PLAN 5x/0.12 PH 0
    Magnification / NA: 5x/0.12
    Immersion: Air
    Correction: Plan Achromat
    Working distance: 14.0 mm
    Product code: 11506303
    AFC compatible: No
    Installed: Yes
    Specialties: phase, dic, uv_transmission
    Notes: FN 22

  • HC PL APO 10x/0.45 AIR
    Leica
    Model: HC PL APO 10x/0.45
    Magnification / NA: 10x/0.45
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 2.8 mm
    Product code: 11506410
    AFC compatible: Yes
    Installed: Yes
    Specialties: dic, uv_transmission
    Notes: FN 25

  • HC PL FLUOTAR L 20x/0.40 CORR AIR
    Leica
    Model: HC PL FLUOTAR L 20x/0.40 CORR
    Magnification / NA: 20x/0.4
    Immersion: Air
    Correction: Fluorite
    Working distance: 7.5 - 6.2 mm
    Product code: 11506242
    AFC compatible: Yes
    Installed: Yes
    Specialties: correction_collar, dic, uv_transmission
    Notes: FN 25

  • HC PL APO 20x/0.70 IMM CORR MULTI-IMMERSION
    Leica
    Model: HC PL APO 20x/0.70 IMM CORR
    Magnification / NA: 20x/0.7
    Immersion: Multi-Immersion
    Correction: Plan Apochromat
    Working distance: 0.66 mm
    Product code: 11506326
    AFC compatible: No
    Installed: Yes
    Specialties: correction_collar, dic, uv_transmission
    Notes: FN 25; W/O/G Immersion

  • HC PL APO 20x/0.80 AIR
    Leica
    Model: HC PL APO 20x/0.80
    Magnification / NA: 20x/0.8
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 0.4 mm
    Product code: 11506529
    AFC compatible: Yes
    Installed: Yes
    Specialties: dic, uv_transmission
    Notes: FN 25

  • HC PL APO 63x/1.40 OIL CS2 OIL
    Leica
    Model: HC PL APO 63x/1.40 OIL CS2
    Magnification / NA: 63x/1.4
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.14 mm
    Product code: 11506350
    AFC compatible: Yes
    Installed: Yes
    Specialties: dic, uv_transmission
    Notes: FN 25

  • HC FLUOTAR L 16x/0.60 IMM CORR VISIR MULTI-IMMERSIONNot installed
    Leica
    Model: HC FLUOTAR L 16x/0.60 IMM CORR VISIR
    Magnification / NA: 16x/0.6
    Immersion: Multi-Immersion
    Correction: Fluorite
    Working distance: 2.5 mm
    Product code: 506533
    AFC compatible: Yes
    Installed: No
    Specialties: correction_collar, ir_transmission, dic
    Notes: FN 20
  • HC PL APO FLUOTAR 10x/0.30 PH 1 AIRNot installed
    Leica
    Model: HC PL APO FLUOTAR 10x/0.30 PH 1
    Magnification / NA: 10x/0.3
    Immersion: Air
    Correction: Plan Apochromat
    Working distance: 11.0 mm
    Product code: 11506507
    AFC compatible: No
    Installed: No
    Specialties: phase, dic, uv_transmission
    Notes: FN 25; from SP5?
  • HC PL APO 100x/1.40 OIL STED OILNot installed
    Leica
    Model: HC PL APO 100x/1.40 OIL STED
    Magnification / NA: 100x/1.4
    Immersion: Oil
    Correction: Plan Apochromat
    Working distance: 0.13 mm
    Product code: 11506316
    AFC compatible: No
    Installed: No
    Specialties: sted, dic, uv_transmission
    Notes: FN 25; from SP5?

Autofocus and triggering

  • Infrared Reflection Autofocus
    Installed: Yes
    Type: Infrared Reflection Autofocus

Optical-path elements

  • Filter Turret
    Cube
    Element type: Turret
    Pos 1: CYR71010 (11525416) — emission 473/22 nm, 539/24 nm, 641/78 nm, 810/80 nm — Quad-band. Excitation: 436/28, 506/21, 578/24, 730/40 nm. Dichroic Cutoffs: 459, 523, 598, 763 nm.
    Pos 2: DFT51010 (11525418) — emission 435/30 nm, 519/25 nm, 594/32 nm, 695/58 nm — Quad-band. Excitation: 391/32, 479/33, 554/24, 638/31 nm. Dichroic Cutoffs: 415, 500, 572, 660 nm.
    Pos 3: CY3 — emission 572/23 nm — LED: 555. Excitation: 546/10 nm. Dichroic Cutoff: 556 nm.
    Pos 4: A594 — emission 630/30 nm — LED: 575. Excitation: 592/21 nm. Dichroic Cutoff: 610 nm.
    Pos 5: EMP_BF (11525000) — Empty position for TL-methods excluding POL, DIC
    Pos 6: EMP+Pol (11525000) — Empty position for TL-methods including POL, DIC, requires external Analyser-slider

  • Standalone Emission Wheel
    Emission
    Element type: Filter Wheel
    Details: External filter wheel; filter exchange time 21 ms (vs ~300 ms by microscope turret)
    Pos 1: 460/80 — emission 460/80 nm
    Pos 2: 535/70 — emission 535/70 nm
    Pos 3: 590/50 — emission 590/50 nm
    Pos 4: 642/80 — emission 642/80 nm
    Pos 5: Empty
    Pos 6: Empty

Latest QC measurements

Laser performance

No recent laser-performance measurements are recorded.

Optical resolution (PSF and alignment)

No recent PSF or chromatic-shift measurements are recorded for these objectives.

Objectives without recent measurements

  • N PLAN 5x/0.12 PH 0 AIR (5x_air)

  • HC PL APO 10x/0.45 AIR (10x_air)

  • HC PL FLUOTAR L 20x/0.40 CORR AIR (20x_air)

  • HC PL APO 20x/0.70 IMM CORR MULTI-IMMERSION (20x_multi)

  • HC PL APO 20x/0.80 AIR (20x_air_2)

  • HC PL APO 63x/1.40 OIL CS2 OIL (63x_oil)

  • HC FLUOTAR L 16x/0.60 IMM CORR VISIR MULTI-IMMERSION (16x_multi)

  • HC PL APO FLUOTAR 10x/0.30 PH 1 AIR (10x_air_2)

  • HC PL APO 100x/1.40 OIL STED OIL (100x_oil)

Information still to confirm

Some details in the facility record are incomplete. If any of these matter for your experiment, please check them with AIC Turku staff.

  • Details needed for this configuration:

  • Software version (software[].version)