📖 Vocabulary dictionary
Use the Canonical ID when authoring controlled fields. Synonyms are rewrite-safe lexical aliases. Classified values are more specific scientific descriptions that map to a broader category but are preserved verbatim and never automatically rewritten.
Jump to a vocabulary
Capabilities & workflows: Adaptive Illumination Methods · Assay Operations · Contrast Methods · Imaging Modes · Measurement Readouts · Modalities · Modules · Non Optical Capabilities · Optical Routes · Workflow Tags
Light paths & optics: Autofocus Types · Branch Modes · Detector Kinds · Endpoint Types · Light Sheet Types · Light Source Kinds · Light Source Roles · Light Source Technologies · Light Source Timing Modes · Ocular Availability · Optical Component Types · Optical Modulator Types · Optical Path Element Types · Optical Path Stage Roles · Phase Mask Types · Scanner Types · Selection Modes · Stage Types · Stand Orientations · Triggering Modes
Objectives: Objective Corrections · Objective Immersion · Objective Specialties
Software: Software Roles
Quality control: Metric Unit · QC Artifact Roles · QC Evaluation Status · QC Measurement Positions · QC Metric Classes · QC Setpoint Units · QC Type
Maintenance: Maintenance Action · Maintenance Reason · Maintenance Status · Service Provider
Record semantics: Identity Semantics
Capabilities & workflows
Adaptive Illumination Methods
Canonical source: vocab/adaptive_illumination_methods.yaml
Used by namespaces: adaptive_illumination_methods
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Confocal/STED switching | confocal_sted_switching |
- | - | Instrument logic for alternating confocal and STED illumination modes. |
| Patterned depletion control | patterned_depletion_control |
- | - | Adaptive control over depletion beam pattern generation. |
| RESCue STED | rescue_sted |
- | - | Adaptive STED strategy suppressing depletion in low-signal regions. |
| ROI-adaptive illumination | roi_adaptive_illumination |
- | - | Enables adaptive illumination based on selected regions of interest. |
Assay Operations
Canonical source: vocab/assay_operations.yaml
Used by namespaces: assay_operations
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| FRAP | frap |
- | - | Fluorescence Recovery After Photobleaching. Photobleaches a region and measures fluorescence recovery over time. Used to quantify mobility, exchange, and binding dynamics. |
| Optogenetics | optogenetics |
- | - | Provides patterned or wavelength-specific light stimulation for genetically encoded light-sensitive actuators. Used to control signaling, neural activity, or cellular behavior during imaging. |
| Photoactivation | photoactivation |
- | - | Activates photoactivatable fluorophores in selected regions or times. Supports pulse-chase imaging, sparse labeling, and dynamic experiments. |
| Photobleaching | photobleaching |
- | - | Selectively bleaches fluorophores in defined regions using high-intensity illumination. Used as a perturbation step in FRAP and related assays, or to reduce background from unwanted signal. |
| Uncaging | uncaging |
- | - | Releases biologically active molecules from a photo-labile cage compound using targeted illumination. Enables spatiotemporally controlled delivery of ligands, signaling molecules, or probes. |
Contrast Methods
Canonical source: vocab/contrast_methods.yaml
Used by namespaces: contrast_methods
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Darkfield | darkfield |
- | - | Collects only scattered light while excluding directly transmitted illumination. Highlights small structures and edges on a dark background. |
| DIC | dic |
- | - | Differential Interference Contrast. Uses polarized beam shearing to create relief-like contrast from optical path gradients. Useful for high-contrast imaging of unstained live samples. |
| Differential Phase Contrast (DPC) | differential_phase_contrast |
- | - | Computational transmitted-light contrast from asymmetric illumination in opposing directions; does not require a phase annulus or phase-contrast objective. |
| Optical sectioning | optical_sectioning |
- | - | Uses patterned illumination and computational processing to suppress out-of-focus signal. Common examples include ApoTome or grid-projection approaches. |
| Phase contrast | phase_contrast |
- | - | Converts phase shifts from transparent specimens into intensity differences. Useful for live, unlabeled cells on standard culture plastic. |
| Polarized light | polarized_light |
- | - | Uses crossed polarizers to generate contrast from birefringent materials like crystals, amyloid fibrils, or muscle fibers. |
| Reflected brightfield | reflected_brightfield |
- | - | Uses incident reflected light rather than transmitted illumination to visualize opaque or thick specimens in brightfield mode. |
| Transmitted brightfield | transmitted_brightfield |
- | - | Uses transmitted white light to image absorption and scattering contrast in unstained specimens. Common for routine morphology and context imaging. |
Imaging Modes
Canonical source: vocab/imaging_modes.yaml
Used by namespaces: imaging_modes
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Confocal point scanning | confocal_point |
- | - | Scans a focused laser spot with a pinhole to reject out-of-focus light. Provides optical sectioning and improved contrast in thick specimens. |
| Confocal spinning disk | confocal_spinning_disk |
- | - | Uses a rotating pinhole disk to image many points in parallel. Enables fast, lower-phototoxicity 3D optical sectioning in live samples. |
| ISM (AiryScan) | ism |
- | - | Image Scanning Microscopy. Uses detector array information in scanning microscopy to improve resolution and signal usage. Airyscan is a common commercial implementation. |
| Light sheet | light_sheet |
- | - | Illuminates samples with a thin sheet orthogonal to detection. Supports gentle, rapid volumetric imaging with reduced out-of-focus exposure. |
| Multiphoton | multiphoton |
- | - | Uses nonlinear excitation, typically with pulsed near-infrared lasers, to excite fluorophores only at the focal volume. Improves deep-tissue imaging and reduces out-of-plane photodamage. |
| RESOLFT | resolft |
- | - | Reversibly Saturable Optical Linear Fluorescence Transitions. Achieves super-resolution using reversibly switchable fluorophores and targeted on/off control. Often allows lower light intensities than depletion-based methods. |
| SIM | sim |
- | - | Structured Illumination Microscopy. Uses shifted illumination patterns and reconstruction to increase resolution and sectioning performance. Suitable for super-resolution with moderate light dose. |
| SMLM | smlm |
- | - | Single-Molecule Localization Microscopy. Builds super-resolved images by localizing sparse single emitters over many frames. Includes methods such as PALM and STORM. |
| STED | sted |
- | - | Stimulated Emission Depletion. Shrinks the effective fluorescence spot using a depletion beam around the excitation focus. Enables sub-diffraction super-resolution imaging. |
| TIRF | tirf |
- | - | Total Internal Reflection Fluorescence. Excites fluorophores with an evanescent field near the coverslip interface. Ideal for membrane-proximal events within roughly the first 100–200 nm. |
| Widefield fluorescence | widefield_fluorescence |
- | - | Illuminates and captures fluorescence from the full field of view without optical sectioning. Best for fast imaging of bright samples and thin specimens. |
Measurement Readouts
Canonical source: vocab/measurement_readouts.yaml
Used by namespaces: measurement_readouts
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| FCS | fcs |
- | - | Fluorescence Correlation Spectroscopy. Analyzes fluorescence intensity fluctuations in a tiny observation volume. Used to estimate concentration, diffusion, and molecular kinetics. |
| FLIM | flim |
- | - | Fluorescence Lifetime Imaging Microscopy. Measures fluorescence decay timing rather than only intensity. Useful for environmental sensing and interaction readouts such as FRET changes. |
| FRET | fret |
- | - | Förster Resonance Energy Transfer. Measures non-radiative energy transfer between donor and acceptor fluorophores at nanometer distances. Used as a readout of molecular proximity or conformational change. |
| Spectral Imaging | spectral_imaging |
- | - | Records fluorescence across wavelength bands rather than fixed channels. Enables unmixing of overlapping fluorophores and autofluorescence separation. |
Modalities
Canonical source: vocab/modalities.yaml
Used by namespaces: modalities
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Atomic Force Microscopy | afm |
AFM |
- | Uses a nanoscale cantilever probe to measure topography and mechanical properties at high spatial resolution. Can be combined with optical microscopy workflows. |
| Confocal (Legacy Route Alias) | confocal |
- | - | Legacy compatibility route alias used in older modality hints. |
| Darkfield | darkfield |
dark field |
- | Collects only scattered light while excluding directly transmitted illumination. Highlights small structures and edges on a dark background. |
| Differential Interference Contrast | dic |
DIC, nomarski |
- | Uses polarized beam shearing to create relief-like contrast from optical path gradients. Useful for high-contrast imaging of unstained live samples. |
| Differential Phase Contrast (DPC) | differential_phase_contrast |
DPC, differential phase contrast |
- | Computational transmitted-light contrast generated from asymmetric illumination in opposing directions. |
| Epi (Legacy Route Alias) | epi |
- | - | Legacy compatibility route alias used in older source/element modality hints. |
| Fluorescence Correlation Spectroscopy | fcs |
FCS |
- | Analyzes fluorescence intensity fluctuations in a tiny observation volume. Used to estimate concentration, diffusion, and molecular kinetics. |
| Fluorescence Lifetime Imaging | flim |
FLIM |
- | Measures fluorescence decay timing rather than only intensity. Useful for environmental sensing and interaction readouts such as FRET changes. |
| Fluorescence Recovery After Photobleaching | frap |
FRAP |
- | Photobleaches a region and measures fluorescence recovery over time. Used to quantify mobility, exchange, and binding dynamics. |
| Förster Resonance Energy Transfer | fret |
FRET |
- | Measures non-radiative energy transfer between donor and acceptor fluorophores at nanometer distances. Used as a readout of molecular proximity or conformational change. |
| Image Scanning Microscopy | ism |
ISM, airyscan |
- | Uses detector array information in scanning microscopy to improve resolution and signal usage. Airyscan implementations are a common form. |
| Impedance Cytometry | impedance_cytometry |
electrical cytometry |
- | Measures electrical impedance changes as cells pass sensing regions to infer size and biophysical properties. Often used for label-free cell phenotyping. |
| Light Sheet | light_sheet |
SPIM, LSFM |
- | Illuminates samples with a thin sheet orthogonal to detection. Supports gentle, rapid volumetric imaging with reduced out-of-focus exposure. |
| Live Cell Imaging | live_cell_imaging |
live imaging, time-lapse live imaging |
- | Refers to imaging workflows optimized for viable cells over time with controlled environment and low phototoxicity. Can be combined with multiple optical modalities. |
| Multiphoton | multiphoton |
two-photon, 2p |
- | Uses nonlinear excitation, typically with pulsed near-infrared lasers, to excite fluorophores only at the focal volume. Improves deep-tissue imaging and reduces out-of-plane photodamage. |
| Phase Contrast | phase_contrast |
phase, ph |
- | Converts phase shifts from transparent specimens into intensity differences. Useful for live, unlabeled cells on standard culture plastic. |
| Photoactivation | photoactivation |
pa, photo-activation |
- | Activates photoactivatable fluorophores in selected regions or times. Supports pulse-chase imaging, sparse labeling, and dynamic experiments. |
| Point-Scanning Confocal | confocal_point |
laser scanning confocal, lsm |
- | Scans a focused laser spot with a pinhole to reject out-of-focus light. Provides optical sectioning and improved contrast in thick specimens. |
| Polarized Light | polarized_light |
polarization |
- | Uses crossed polarizers to generate contrast from birefringent materials like crystals, amyloid fibrils, or muscle fibers. |
| Reflected Brightfield | reflected_brightfield |
epi brightfield, reflected light brightfield |
- | Uses incident reflected light rather than transmitted illumination to visualize opaque or thick specimens in brightfield mode. |
| RESOLFT | resolft |
RESOLFT |
- | Achieves super-resolution using reversibly switchable fluorophores and targeted on/off control. Often allows lower light intensities than depletion-based methods. |
| Second Harmonic Generation | shg |
SHG |
- | Label-free nonlinear imaging modality generating signal at half the excitation wavelength from non-centrosymmetric structures such as collagen. |
| Single-Molecule Localization Microscopy | smlm |
SMLM, PALM/STORM |
- | Builds super-resolved images by localizing sparse single emitters over many frames. Includes methods such as PALM and STORM. |
| Single-Particle Tracking | spt |
SPT, single molecule tracking |
- | Tracks individual particles or molecules over time to quantify motion and dynamics. Used for diffusion, transport, and interaction studies. |
| Spectral Imaging | spectral_imaging |
lambda imaging, spectral detection |
- | Records fluorescence across wavelength bands rather than fixed channels. Enables unmixing of overlapping fluorophores and autofluorescence separation. |
| Spinning-Disk Confocal | confocal_spinning_disk |
SDC, spinning disk |
- | Uses a rotating pinhole disk to image many points in parallel. Enables fast, lower-phototoxicity 3D optical sectioning in live samples. |
| Stimulated Emission Depletion | sted |
STED |
- | Shrinks the effective fluorescence spot using a depletion beam around the excitation focus. Enables sub-diffraction super-resolution imaging. |
| Structured Illumination Microscopy | sim |
SIM, structured illumination |
- | Uses shifted illumination patterns and reconstruction to increase resolution and sectioning performance. Suitable for super-resolution with moderate light dose. |
| Structured Optical Sectioning | optical_sectioning |
apotome, grid projection |
- | Uses patterned illumination and computational processing to suppress out-of-focus signal. Common examples include ApoTome or grid-projection approaches. |
| Third Harmonic Generation | thg |
THG |
- | Label-free nonlinear imaging modality generating signal at one-third the excitation wavelength, often sensitive to refractive-index discontinuities. |
| TIRF-SIM | tirf_sim |
TIRF-SIM, 2D TIRF-SIM |
- | Combines Total Internal Reflection with Structured Illumination for high-speed, high-contrast, super-resolved surface imaging. |
| Total Internal Reflection Fluorescence | tirf |
TIRF |
- | Excites fluorophores with an evanescent field near the coverslip interface. Ideal for membrane-proximal events within roughly the first 100–200 nm. |
| Transmitted (Legacy Route Alias) | transmitted |
- | - | Legacy compatibility transmitted-route alias used in older modality hints. |
| Transmitted Brightfield | transmitted_brightfield |
brightfield, bf |
- | Uses transmitted white light to image absorption and scattering contrast in unstained specimens. Common for routine morphology and context imaging. |
| Widefield Fluorescence | widefield_fluorescence |
epifluorescence, widefield epi |
- | Illuminates and captures fluorescence from the full field of view without optical sectioning. Best for fast imaging of bright samples and thin specimens. |
Modules
Canonical source: vocab/modules.yaml
Used by namespaces: modules
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| 3D-SIM Module | 3d_sim |
Blaze R2 3D-SIM |
- | Structured illumination module for three-dimensional super-resolution imaging with enhanced axial and lateral detail. |
| Acousto-Optical Beam Splitter (AOBS) | aobs |
AOBS |
- | Acousto-optic beam splitting component for dynamic wavelength selection and flexible excitation-emission routing. |
| Adaptive Optics | adaptive_optics |
ao |
- | Corrects sample- or system-induced aberrations using deformable elements and feedback. Improves resolution and signal quality, especially in deep or heterogeneous samples. |
| AFM Module | afm_module |
AFM |
- | Atomic force microscopy integration module for combining topographical or mechanical probing with optical imaging. |
| AiryScan Detector/Module | airyscan |
AiryScan |
- | Detector and reconstruction module that improves confocal sensitivity and resolution via multi-element signal collection. |
| Easy3D STED | easy3d_sted |
Easy3D_STED |
- | STED imaging enhancement for simplified three-dimensional super-resolution acquisition with optimized 3D depletion beam handling. |
| Environmental Enclosure | environmental_enclosure |
Environmental Enclosure, Temperature Control, environmental_tolerance, Live Cell Option |
- | Enclosure and control accessories that stabilize temperature and related conditions for live-cell compatibility. |
| External Camera Port | camera_port |
trinocular head, c-mount port, photo port |
- | An available optical port (e.g., trinocular head or side port) and c-mount adapter allowing users to attach external or third-party imaging devices. |
| FCS Module | fcs |
correlation module, FCS |
- | Instrumentation support for fluorescence fluctuation measurements and autocorrelation analysis. Enables diffusion and concentration quantification in tiny volumes. |
| FLIM Module | flim |
lifetime module, FLIM |
- | Adds lifetime-resolved detection and analysis to fluorescence imaging. Enables contrast based on fluorophore decay kinetics. |
| FRAP Module | frap |
photobleach module, FRAP |
- | Hardware or software capability to perform targeted photobleaching for recovery measurements. Typically controls ROI bleaching power and timing. |
| Hardware Autofocus | hardware_autofocus |
focus lock, z-drift compensation |
- | Active focus stabilization that compensates focus drift during acquisition. Common implementations include reflected-light focus locks such as Definite Focus or PFS. |
| Hardware Triggering | hardware_triggering |
triggering kit, device synchronization, ttl triggering |
- | Hardware-level synchronization module for deterministic timing between cameras, scanners, lasers, shutters, and other devices during fast or complex acquisitions. |
| High-Performance Workstation | workstation |
workstation |
- | Dedicated compute workstation for acquisition control, rapid image processing, and handling large microscopy datasets. |
| Impedance Module | impedance_module |
impedance_module |
- | Module supporting impedance-based measurement workflows alongside optical imaging for complementary sample characterization. |
| Incubation | incubation |
environmental chamber, temp/co2 control |
- | Environmental control module for temperature, CO2, and often humidity around the specimen. Supports physiologic conditions during long-term live imaging. |
| Microfluidics / Perfusion | microfluidics |
perfusion pump |
- | Hardware for controlled delivery of media or drugs to the sample during live imaging. |
| Motorized Stage | motorized_stage |
multiposition, xy stage |
- | Automated XY stage enabling multi-position imaging, large-area tiling, and multi-well plate screening. |
| Optogenetics | optogenetics |
photo-stimulation |
- | Provides patterned or wavelength-specific stimulation for light-controlled biological perturbations. Used to control signaling or neural activity during imaging. |
| Photoactivation Module | photoactivation |
photo-manipulation, pa module |
- | Capability to activate or uncage photoresponsive probes in defined regions. Used for selective labeling and dynamic tracking experiments. |
| Piezo Z-Stage | piezo_z_stage |
piezo_z_stage |
- | High-precision piezoelectric Z positioning module for rapid and repeatable axial movement during fine focus control and volume imaging. |
| RESCue STED | rescue_sted |
RESCue_STED |
- | Adaptive STED mode that limits depletion exposure to signal-bearing regions to reduce photobleaching and phototoxicity. |
| Ring TIRF Module | ring_tirf |
Ring TIRF |
- | TIRF implementation using ring illumination to improve field uniformity near the coverslip interface. |
| SIM Module | sim_module |
SIM |
- | Structured illumination imaging module enabling resolution enhancement beyond diffraction-limited widefield microscopy. |
| TIRF Module | tirf |
TIRF |
- | Dedicated total internal reflection fluorescence illumination path for near-membrane imaging. |
| Universal Vessel Holder | universal_vessel_holder |
Universal vessel holder |
- | Adaptable sample holder supporting multiple dish, slide, or plate formats within a single imaging setup. |
Non Optical Capabilities
Canonical source: vocab/non_optical_capabilities.yaml
Used by namespaces: non_optical_capabilities
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| AFM | afm |
- | - | Atomic Force Microscopy. Uses a nanoscale cantilever probe to measure topography and mechanical properties at high spatial resolution. Can be combined with optical microscopy workflows. |
| Impedance cytometry | impedance_cytometry |
- | - | Measures electrical impedance changes as cells pass sensing regions to infer size and biophysical properties. Often used for label-free cell phenotyping. |
Optical Routes
Canonical source: vocab/optical_routes.yaml
Used by namespaces: optical_routes
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Light sheet | light_sheet |
SPIM, LSFM, light sheet fluorescence microscopy |
- | Light-sheet illumination and detection route family, including SPIM variants. |
| Multiphoton | multiphoton |
two-photon, 2p, nonlinear microscopy, non-linear microscopy |
- | Multiphoton excitation route family. |
| Point-scanning confocal | confocal_point |
LSM, laser scanning confocal, CLSM, point scanning confocal |
- | Broad point-scanning confocal route family, including point-scanning super-resolution or readout workflows implemented on the confocal scanner path. |
| Reflected light | reflected_light |
incident light, epi brightfield, reflected illumination |
- | Reflected or incident-light route family used to illuminate opaque or thick specimens from above the sample. |
| Spinning-disk confocal | confocal_spinning_disk |
SDC, spinning disk confocal, Nipkow disk confocal |
- | Broad spinning-disk confocal route family, including CSU-W1 and Borealis spinning disk implementations. |
| Transmitted light | transmitted_light |
transmitted, brightfield, diascopic, transmitted illumination |
- | Broad transmitted-light route family covering brightfield and transmitted contrast methods. |
| Widefield fluorescence | widefield_fluorescence |
widefield, epifluorescence, epi fluorescence, widefield epi |
- | Broad widefield/epi fluorescence route family, including TIRF and widefield-based localization or structured illumination acquisitions when implemented through the widefield fluorescence path. |
Workflow Tags
Canonical source: vocab/workflow_tags.yaml
Used by namespaces: workflow_tags
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| High-content screening | high_content_screening |
- | - | Automated acquisition and analysis across many samples, fields, or conditions. Typically involves motorized stages, plate formats, and quantitative readout pipelines. |
| Live cell imaging | live_cell_imaging |
- | - | Imaging workflows optimized for viable cells over time with controlled environment and low phototoxicity. Can be combined with multiple optical modalities. |
| Single-molecule imaging | single_molecule |
- | - | Workflows that detect and analyze individual fluorescent molecules. Requires low labeling densities and sensitive detection conditions. |
| Single-particle tracking | single_particle_tracking |
spt |
- | Tracks individual particles or molecules over time to quantify motion and dynamics. Used for diffusion, transport, and interaction studies. |
| Super-resolution | super_resolution |
- | - | Workflows that achieve spatial resolution beyond the classical diffraction limit. Encompasses techniques including STED, RESOLFT, SIM, and SMLM. |
Light paths & optics
Autofocus Types
Canonical source: vocab/autofocus_types.yaml
Used by namespaces: autofocus_types
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Capacitance-Based Autofocus | capacitance_based |
capacitive autofocus |
- | Autofocus using capacitive sensing to track objective-to-sample distance and stabilize focus. |
| Contrast-Based Autofocus | contrast_based |
image-based autofocus, software autofocus, focus metric |
- | Autofocus that optimizes image sharpness or contrast directly from the captured image signal. |
| Infrared Reflection Autofocus | infrared_reflection |
PFS, perfect focus, Nikon Perfect Focus, Nikon Perfect Focus System, Definite Focus, Zeiss Definite Focus, Leica AFC, Olympus ZDC |
- | Hardware autofocus based on infrared reflection from the coverslip or sample interface to compensate focus drift. |
| Laser Reflection Autofocus | laser_reflection |
reflection autofocus, hardware focus lock |
- | Hardware autofocus that uses reflected laser light to maintain focal position during imaging. |
| Other | other |
custom, misc |
- | Hardware autofocus implementation not covered by the predefined terms. |
Branch Modes
Canonical source: vocab/branch_modes.yaml
Used by namespaces: branch_modes
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Passthrough | passthrough |
- | - | Branch does not materially alter routing beyond target selection. |
| Reflected | reflected |
- | - | Branch follows the reflected path of a selector or splitter. |
| Selected | selected |
- | - | Branch is selected by state rather than by transmitted/reflected optics. |
| Transmitted | transmitted |
- | - | Branch follows the transmitted path of a selector or splitter. |
Detector Kinds
Canonical source: vocab/detector_kinds.yaml
Used by namespaces: detector_kinds
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Avalanche Photodiode | apd |
APD |
- | Semiconductor point detector with internal gain and fast timing response. Used in photon counting and correlation-based measurements. |
| Charge-Coupled Device | ccd |
CCD |
- | Area detector technology with historically strong image quality and uniformity. Still used in some fluorescence and brightfield setups. |
| Electron-Multiplying CCD | emccd |
EMCCD |
- | CCD detector with on-chip gain for very low-light imaging. Useful for single-molecule and fast, dim signal applications. |
| GaAsP Photomultiplier Tube | gaasp_pmt |
GaAsP_PMT, gaasp detector |
- | PMT using gallium arsenide phosphide photocathodes with improved quantum efficiency over conventional PMTs. Useful for low-light confocal imaging. |
| Hybrid Detector | hyd |
HyD, hybrid pmt |
- | Combines features of PMTs and avalanche gain to provide high sensitivity and fast response. Frequently used in modern confocal systems. |
| Photomultiplier Tube | pmt |
PMT |
- | Highly sensitive point detector that amplifies photoelectrons through a dynode chain. Common in confocal detection paths. |
| Scientific CMOS | scmos |
sCMOS |
- | Low-noise, high-speed area detector with large field of view and high dynamic range. Common for widefield, spinning-disk, and fast live imaging. |
| SPAD Array | spad |
- | - | Single-Photon Avalanche Diode array. Provides exceptional timing resolution and single-photon sensitivity, crucial for advanced FLIM. |
| Standard CMOS | cmos |
CMOS |
- | Standard digital camera sensor. Commonly used in routine brightfield, color histology, or basic fluorescence. |
Endpoint Types
Canonical source: vocab/endpoint_types.yaml
Used by namespaces: endpoint_types
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Camera port | camera_port |
camera port, cameraport |
- | Passive camera port endpoint. |
| Detector | detector |
camera, pmt, hyd, apd, spad |
- | Detection endpoint represented by an electronic detector channel. |
| Eyepiece | eyepiece |
ocular, eyepieces, binocular, trinocular |
- | Ocular/eyepiece endpoint for visual observation. |
Light Sheet Types
Canonical source: vocab/light_sheet_types.yaml
Used by namespaces: light_sheet_types
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Airy Beam Light Sheet | airy_beam |
- | - | Airy-beam light sheet implementation. |
| diSPIM | di_spim |
- | - | Dual-view inverted selective-plane illumination microscopy. |
| Lattice Light Sheet | lattice |
- | - | Structured lattice light-sheet implementation. |
| Other | other |
- | - | Light-sheet architecture not covered by the predefined terms. |
| SCAPE | scape |
- | - | Swept confocally aligned planar excitation. |
| Single-Objective Light Sheet | single_objective |
- | - | Single-objective light-sheet architecture. |
| Standard SPIM | standard_spim |
- | - | Conventional selective-plane illumination geometry. |
Light Source Kinds
Canonical source: vocab/light_source_kinds.yaml
Used by namespaces: light_source_kinds
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Arc Lamp | arc_lamp |
mercury lamp, xenon lamp |
- | Broad-spectrum lamp, such as mercury or xenon, used with filter sets for fluorescence excitation. Historically common on epifluorescence microscopes. |
| Halogen Lamp | halogen_lamp |
tungsten-halogen, quartz halogen |
- | Continuous-spectrum tungsten-halogen illumination, primarily for transmitted light imaging. Common for brightfield and contrast techniques. |
| Laser | laser |
single-line laser, laser_diode, laser_dpss, laser (diode), laser (DPSS), dpss |
- | Provides coherent, narrowband excitation at specific wavelengths. Common for fluorescence imaging requiring high irradiance and precise line selection. |
| LED | led |
light-emitting diode |
- | Solid-state light source with stable output and fast switching. Widely used for widefield fluorescence and transmitted illumination. |
| Metal Halide Lamp | metal_halide |
hxp |
- | Broad-spectrum lamp coupled via a liquid light guide. Provides intense, stable excitation for standard widefield fluorescence. |
| Pulsed Near-IR Laser | multiphoton_laser |
- | ti_sapphire, ti:sapphire |
Femtosecond pulsed laser tunable in the near-infrared. Used for deep-tissue multiphoton excitation. |
| Supercontinuum Source | supercontinuum |
white supercontinuum laser |
- | Broadband laser-like source generated by nonlinear spectral broadening. Provides highly flexible, selectable excitation bands. |
| White Light Laser | white_light_laser |
WLL |
- | Broadband laser source with tunable output wavelengths selected by software or optics. Supports flexible excitation across many fluorophores. |
Light Source Roles
Canonical source: vocab/light_source_roles.yaml
Used by namespaces: light_source_roles
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Activation | activation |
- | - | Photoactivation or photoswitching source. |
| Alignment | alignment |
- | - | Alignment or service beam not used as primary excitation/depletion. |
| Depletion | depletion |
sted_depletion, sted |
- | Source that depletes or switches off fluorescence outside the effective focus. The mechanism belongs to the technique: stimulated emission under STED, reversible photoswitching under RESOLFT. |
| Excitation | excitation |
excitation_laser |
- | Primary fluorophore excitation source. |
| Reflected Illumination | reflected_illumination |
epi illumination, incident illumination |
- | Source provides reflected-light illumination onto the sample from the objective or an epi/top-light path. |
| Transmitted Illumination | transmitted_illumination |
transmitted, brightfield, diascopic, dia |
- | Diascopic or transmitted-light illumination source used for brightfield, phase contrast, darkfield, or DIC. |
Light Source Technologies
Canonical source: vocab/light_source_technologies.yaml
Used by namespaces: light_source_technologies
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Diode | diode |
- | - | Diode laser or diode-driven light source. |
| DPSS | dpss |
- | - | Diode-pumped solid-state laser. |
| LED Array | led_array |
- | - | Multi-channel LED engine or array. |
| Other | other |
- | - | Technology subtype not covered by the predefined terms. |
| Supercontinuum | supercontinuum |
- | - | Supercontinuum broadband source. |
| Ti:Sapphire | ti_sapphire |
- | - | Tunable femtosecond Ti:Sapphire source. |
Light Source Timing Modes
Canonical source: vocab/light_source_timing_modes.yaml
Used by namespaces: light_source_timing_modes
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Continuous Wave | cw |
continuous, continuous_wave |
- | Continuous-wave operation without pulse train timing metadata. |
| Pulsed | pulsed |
pulse, pulse_train |
- | Pulsed operation with pulse width and repetition rate metadata. |
Ocular Availability
Canonical source: vocab/ocular_availability.yaml
Used by namespaces: ocular_availability
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Binocular | binocular |
2 eyepiece, two eyepiece, biocular |
- | Microscope stand provides two-eyepiece direct viewing. |
| Camera-only | camera_only |
camera only, digital only, no eyepieces |
- | No direct visual eyepiece path; observation is camera-mediated only. |
| Trinocular | trinocular |
3 port, trinoc, trinocular head |
- | Microscope stand provides binocular viewing plus a camera port. |
| Unknown | unknown |
unspecified, not documented |
- | Eyepiece availability is not yet documented. |
Optical Component Types
Canonical source: vocab/optical_component_types.yaml
Used by namespaces: optical_component_types
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Analyzer | analyzer |
- | - | Polarization analyzer element used in DIC or polarized-light detection paths. |
| Bandpass | bandpass |
- | - | Requires center_nm and width_nm. |
| Block | block |
- | - | A 100% blocking/shutter position. |
| Dichroic | dichroic |
- | - | Requires cutoffs_nm array. |
| Empty | empty |
- | - | An explicitly empty slot. |
| Filter Cube | filter_cube |
- | - | Linked excitation/dichroic/emission cube or filter-set assembly installed as a single selectable position. Supports explicit excitation_filter, dichroic, and emission_filter sub-component objects for authoritative spectral authoring. |
| Longpass | longpass |
- | - | Requires cut_on_nm. |
| Mirror | mirror |
- | - | A 100% reflective surface. |
| Multiband Bandpass | multiband_bandpass |
- | - | Bandpass component containing multiple pass bands. |
| Multiband Dichroic | multiband_dichroic |
- | - | Requires cutoffs_nm array. |
| Neutral Density | neutral_density |
- | - | Broadband neutral-density attenuator. Spectral shape is preserved even if total intensity is reduced. |
| Notch | notch |
- | - | Requires center_nm and width_nm. |
| Passthrough | passthrough |
- | - | Explicit slot that does not materially alter spectral routing. |
| Polychroic | polychroic |
- | - | Synonym for multiband. |
| Shortpass | shortpass |
- | - | Requires cut_off_nm. |
Optical Modulator Types
Canonical source: vocab/optical_modulator_types.yaml
Used by namespaces: optical_modulator_types
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Acousto-Optic Deflector | aod |
- | - | AOD-based beam steering or modulation device. |
| Deformable Mirror | deformable_mirror |
- | - | Adaptive mirror with controllable surface profile. |
| Electro-Optic Modulator | eom |
- | - | Electro-optic phase or amplitude modulator. |
| Other | other |
- | - | Other optical modulator type not covered above. |
| Phase Plate | phase_plate |
- | - | Static phase plate for beam shaping. |
| Spatial Light Modulator | slm |
- | - | Programmable spatial light modulator used for phase shaping. |
| Vortex Plate | vortex_plate |
- | - | Vortex phase element used for donut beam generation. |
Optical Path Element Types
Canonical source: vocab/optical_path_element_types.yaml
Used by namespaces: optical_path_element_types
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Dual View | dual_view |
- | - | Two-way image splitter with explicit branch targets. |
| Emission Splitter | emission_splitter |
- | - | Emission-path splitter that routes detected light into explicit branches. |
| Filter Wheel | filter_wheel |
- | - | Discrete wheel or carousel that selects one optical position at a time. |
| Fixed | fixed |
- | - | Fixed optical element with no active selection mechanism. |
| Image Splitter | image_splitter |
- | - | Image-splitting element such as a dual-view or quad-view splitter. |
| Quad View | quad_view |
- | - | Four-way image splitter with explicit branch targets. |
| Selector | selector |
- | - | Selection element that routes light into one or more explicit branches. |
| Slider | slider |
- | - | Linear slider with one active position at a time. |
| Spectral Array | spectral_array |
- | - | Multi-band or array-based spectral selection element. |
| Spectral Slider | spectral_slider |
- | - | Spectral slit/slider mechanism used to define wavelength windows. |
| Splitter | splitter |
- | - | Optical splitter with explicit branch definitions and endpoint targets. |
| Tunable | tunable |
- | - | Continuously tunable optical element such as an AOTF or spectral selector. |
| Turret | turret |
- | - | Rotary turret or cube carousel. |
Optical Path Stage Roles
Canonical source: vocab/optical_path_stage_roles.yaml
Used by namespaces: optical_path_stage_roles
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Analyzer | analyzer |
- | - | Element represents a DIC/polarization analyzer stage in the detection path. |
| Cube | cube |
- | - | Element represents a linked cube/filter-set assembly. |
| Dichroic | dichroic |
- | - | Element participates in dichroic routing around the sample plane. |
| Emission | emission |
- | - | Element participates in the detection path after the sample plane. |
| Excitation | excitation |
- | - | Element participates in the illumination path before the sample plane. |
| Splitter | splitter |
- | - | Element explicitly branches or selects downstream routes/endpoints. |
Phase Mask Types
Canonical source: vocab/phase_mask_types.yaml
Used by namespaces: phase_mask_types
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| 3D-STED | 3d_sted |
- | - | Combined mask strategy for three-dimensional STED depletion. |
| Bottle | bottle |
- | - | Bottle-beam phase mask for axial confinement. |
| Top-Hat | top_hat |
- | - | Top-hat phase profile for beam shaping. |
| Vortex | vortex |
- | - | Azimuthal vortex phase mask for donut-shaped depletion beam. |
| Z-STED | z_sted |
- | - | Phase mask optimized for axial STED depletion. |
Scanner Types
Canonical source: vocab/scanner_types.yaml
Used by namespaces: scanner_types
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Acousto-Optic Scanner | acousto_optic |
AOD, acousto-optic deflector |
- | Deflects beams using acousto-optic elements for rapid, inertia-free positioning. Useful for fast random-access scanning patterns. |
| Galvanometric Scanner | galvo |
galvanometer, galvo mirrors, point_scanning |
- | Uses galvanometer mirrors to steer the excitation beam across the sample. Common in point-scanning confocal systems with flexible scan control. |
| No Scanner | none |
camera-based |
- | No dedicated beam scanner is used for image formation. Typical of camera-based widefield modalities. |
| Polygon Scanner | polygon |
polygon mirror |
- | Uses a rotating polygon mirror for rapid beam deflection. Often used in high-speed scanning implementations. |
| Resonant Scanner | resonant |
resonant galvo |
- | Uses a resonant oscillating mirror for very high line rates. Enables faster imaging than standard galvo scanning, often with reduced dwell time. |
| Spinning Disk Scanner | spinning_disk |
nipkow disk, sdc scanner |
- | Uses rotating pinhole disks to scan multiple points in parallel. Supports high-speed confocal imaging with lower phototoxicity. |
| Stage Scanning | stage_scanning |
sample scanning |
- | Forms images by moving the sample stage relative to fixed optics or beam position. Common in slide scanning and some large-area acquisitions. |
| Tandem Scanner (Galvo/Resonant) | tandem |
galvanometric_and_resonant |
- | Dual-mode scanning configuration that supports switching between galvanometric and resonant scanning behavior. |
Selection Modes
Canonical source: vocab/selection_modes.yaml
Used by namespaces: selection_modes
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Exclusive | exclusive |
- | - | Exactly one branch should be selected at a time. |
| Fixed | fixed |
- | - | No user branch selection is required; the branch state is fixed. |
| Multiple | multiple |
- | - | More than one branch may be active simultaneously. |
Stage Types
Canonical source: vocab/stage_types.yaml
Used by namespaces: stage_types
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Manual Stage | manual |
manual focus, manual drive |
- | Manual stage or focus drive without motorized control. |
| Motorized XY Stage | xy_motorized |
motorized_xy, xy stage, xy motorized |
- | Motorized translational stage for automated X/Y movement across the sample plane. |
| Other | other |
custom, misc |
- | Stage or focus-drive type not covered by the predefined terms. |
| Z Piezo Stage | z_piezo |
piezo, piezo stage, piezo z |
- | Piezo-driven stage or objective positioner used for rapid, precise axial stepping. |
Stand Orientations
Canonical source: vocab/stand_orientations.yaml
Used by namespaces: stand_orientations
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Benchtop | benchtop |
- | - | Compact benchtop imaging instrument. |
| Inverted | inverted |
- | - | Inverted microscope stand. |
| Other | other |
- | - | Stand orientation not covered by the predefined terms. |
| Stereo | stereo |
- | - | Stereo / dissecting microscope stand. |
| Upright | upright |
- | - | Conventional upright microscope stand. |
Triggering Modes
Canonical source: vocab/triggering_modes.yaml
Used by namespaces: triggering_modes
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Hardware Triggering | hardware |
ttl, external trigger |
- | Synchronization is driven primarily by hardware timing signals such as TTL triggers. |
| Mixed Triggering | mixed |
hybrid |
- | Synchronization uses a combination of hardware triggers and software orchestration. |
| Software Triggering | software |
computer controlled |
- | Synchronization is driven primarily through software control rather than dedicated timing signals. |
Objectives
Objective Corrections
Canonical source: vocab/objective_corrections.yaml
Used by namespaces: objective_corrections
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Achromat | achromat |
achro |
- | Basic objective correction class with limited chromatic and spherical correction. Often used for standard, non-demanding imaging tasks. |
| Apochromat | apochromat |
apo |
- | Objective with strong chromatic and spherical aberration correction, typically over multiple colors. May not guarantee full field flatness. |
| Fluorite | fluorite |
semi-apo, fluor |
- | Objective class with improved color correction and transmission relative to basic achromats. Common compromise between performance and cost. |
| Plan Achromat | plan_achromat |
plan achro |
- | Objective with flat field correction and basic chromatic correction for two wavelengths. Suitable for routine brightfield and general imaging. |
| Plan Apochromat | plan_apochromat |
plan-apo, plan apo, super apochromat, super_apochromat, uplsapo |
- | High-end objective with flat field correction and strong chromatic/spherical correction across wavelengths. Preferred for quantitative, multicolor imaging. |
| Plan Fluorite | plan_fluorite |
plan fluor, plan fluorite |
- | Objective with flat field correction and moderate-to-high aberration correction, typically between achromat and apochromat classes. |
Objective Immersion
Canonical source: vocab/objective_immersion.yaml
Used by namespaces: objective_immersion
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Air | air |
dry |
- | Objective is used without immersion liquid, with air between front lens and coverslip. Common for lower magnification or long working distance imaging. |
| Dipping | dipping |
water dipping, dipping objective |
- | Front lens is dipped directly into sample medium without coverslip contact. Common in electrophysiology and in vivo preparations. |
| Glycerol | glycerol |
glycerin |
- | Uses glycerol immersion to better match intermediate refractive indices. Useful for some cleared or thick biological specimens. |
| Multi-Immersion | multi |
multi immersion |
- | Objective is designed to operate with multiple immersion media, often with correction adjustments. Provides flexibility across sample types. |
| Oil | oil |
oil immersion |
- | Uses immersion oil to better match refractive index and increase numerical aperture. Common for high-resolution fluorescence objectives. |
| Silicone | silicone |
silicone oil |
- | Uses silicone oil immersion with refractive index close to tissue, improving deep imaging stability. Often used in live or thick sample imaging. |
| Water | water |
water immersion |
- | Uses water as immersion medium for improved index matching in aqueous samples. Useful for live-cell and deeper tissue imaging. |
Objective Specialties
Canonical source: vocab/objective_specialties.yaml
Used by namespaces: objective_specialties
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Cleared Tissue Imaging | cleared_tissue_imaging |
- | Cleared Tissue Imaging (CLARITY and TDE) |
Objective suitable for imaging optically cleared tissue samples. |
| Correction Collar | correction_collar |
Correction Ring, Correction Ring (CORR), correction collar |
correction collar for 0-1.5mm glass, correction ring for 0.17 mm cover glass |
Objective is explicitly described as having an adjustable correction collar or correction ring. |
| Cover Glass Specification | cover_glass_specification |
- | cover glass 0.17, cover glass 0.75 |
Objective has an explicit cover-glass thickness or specification; this does not imply an adjustable correction collar. |
| Coverslip Correction | coverslip_correction |
- | - | Objective is explicitly described as supporting coverslip correction. |
| DIC | dic |
DIC |
DIC (Suitable), DIC (Recommended), dic compatible, DIC N1, DIC N1 (Suitable), DIC N2, DIC N2 (Suitable), DIC N2 (Recommended), DIC Prism A, DIC Prism B, DIC Prism C, DIC Prism D, DIC Prism E |
Objective supports differential interference contrast. |
| IR Transmission | ir_transmission |
- | UV-VIS-IR, (UV)VIS-IR, VISIR, IR-compatible to 1300 nm |
Objective optimized for IR / multiphoton transmission. |
| Long Working Distance | long_working_distance |
LWD, ELWD |
ELWD ADM |
Objective designed for increased working distance. |
| Multi-immersion | multi_immersion |
- | Multi-immersion, Multi-immersion (BABB/W/Glyc/Sil-Oil) |
Objective is explicitly described as supporting multiple immersion media; this does not imply a correction collar. |
| Other | other |
- | - | Specialty not covered by the predefined terms. |
| Phase | phase |
- | Ph1, phase contrast (ph), phase contrast (ph 0), phase contrast (ph 1), phase contrast (ph1 dl), phase contrast (ph2), phase contrast (ext ph4), phase contrast (ph1), phase compatible |
Objective supports phase-contrast use. |
| Polarization | polarization |
- | Polarization (Possible), Polarization (Suitable) |
Objective supports polarization imaging workflows. |
| SIM | sim |
SIM |
- | Objective suitable for structured illumination microscopy workflows. |
| STED | sted |
STED |
- | Objective suitable for stimulated emission depletion microscopy workflows. |
| TIRF | tirf |
TIRF |
- | Objective optimized for total internal reflection fluorescence imaging. |
| UV Transmission | uv_transmission |
- | UV ≥340 nm, UV ≥365 nm |
Objective optimized for UV transmission. |
| Water Dipping | water_dipping |
- | - | Objective designed for direct immersion into sample medium. |
Software
Software Roles
Canonical source: vocab/software_roles.yaml
Used by namespaces: software_roles
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Acquisition | acquisition |
capture |
- | Software used for instrument control and image acquisition. |
| Analysis | analysis |
quantification |
- | Software used for quantitative analysis, fitting, and measurement. |
| Hardware Control | hardware_control |
control |
- | Software used to control ancillary hardware subsystems. |
| Other | other |
- | - | Software that does not fit acquisition, processing, analysis, or hardware control roles. |
| Processing | processing |
reconstruction, deconvolution, post_processing |
- | Software used for post-acquisition image processing and reconstruction workflows. |
Quality control
Metric Unit
Canonical source: vocab/metric_unit.yaml
Used by namespaces: metric_unit
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Ampere | A |
amp, amps, ampere |
- | Electrical current expressed in amperes. |
| Degrees Celsius | celsius |
C, °C |
- | Temperature expressed in degrees Celsius. |
| Hour | h |
hour, hours |
- | Elapsed time expressed in hours. |
| Micrometer | um |
- | - | Length expressed in micrometers. |
| Milliwatt | mW |
- | - | Optical power expressed in milliwatts. |
| Nanometer | nm |
- | - | Length expressed in nanometers. |
| Percent | percent |
- | - | Percentage value represented in %. |
| Pixel | px |
pixel, pixels |
- | Image-space distance or count expressed in pixels. |
| Unitless | unitless |
- | - | Dimensionless value with no physical unit. |
QC Artifact Roles
Canonical source: vocab/qc_artifact_roles.yaml
Used by namespaces: artifact_role, qc_artifact_roles
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Other | other |
- | - | Artifact role not captured by existing categories; describe in details. |
| Preview | preview |
- | - | Rendered quick-look artifact intended for visual inspection. |
| Raw Image | raw_image |
- | - | Original unprocessed image data artifact produced by the QC run. |
| Report | report |
- | - | Human-readable summary report such as PDF or HTML output. |
| Table | table |
- | - | Tabular artifact such as CSV, TSV, or spreadsheet-like export. |
QC Evaluation Status
Canonical source: vocab/qc_evaluation_status.yaml
Used by namespaces: evaluation_status, qc_evaluation_status
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Fail | fail |
- | - | Measured value is out of acceptable range and requires follow-up action. |
| Pass | pass |
- | - | Measured value meets expected criteria or threshold. |
| Warn | warn |
- | - | Measured value is borderline or drifting and should be monitored. |
QC Measurement Positions
Canonical source: vocab/qc_measurement_positions.yaml
Used by namespaces: measurement_positions, qc_measurement_positions
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| At Objective | at_objective |
- | - | Measured at or near the objective/sample plane where illumination reaches the specimen. |
| Fiber Output | fiber_output |
- | - | Measured at the optical fiber output before downstream beam delivery optics. |
| Pre-objective | pre_objective |
- | - | Measured in the optical path before the objective lens. |
QC Metric Classes
Canonical source: vocab/qc_metric_classes.yaml
Used by namespaces: qc_metric_classes
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Absolute or relative illumination intensity | laser_power |
- | - | Class of illumination metrics describing measured excitation intensity or power output. |
| Axial (Z) Full Width at Half Maximum | fwhm_axial |
- | - | Class of resolution metrics describing axial point-spread width along the optical axis. |
| Baseline noise of the camera/detector | detector_dark_noise |
- | - | Class of detector metrics describing dark signal and baseline electronic noise without illumination. |
| Error or variance in stage repositioning | stage_repeatability |
- | - | Class of positioning metrics describing precision and repeatability of stage return to target coordinates. |
| Flatness of the field of view | illumination_uniformity |
- | - | Class of illumination metrics describing spatial uniformity of intensity across the field. |
| Lateral (XY) Full Width at Half Maximum | fwhm_lateral |
- | - | Class of resolution metrics describing lateral point-spread width in the imaging plane. |
| Signal-to-noise or contrast ratio | signal_to_noise |
- | - | Class of image quality metrics describing signal strength relative to background noise or contrast. |
| Spatial offset between color channels | chromatic_shift |
- | - | Class of alignment metrics describing chromatic displacement between channels in multicolor imaging. |
QC Setpoint Units
Canonical source: vocab/qc_setpoint_units.yaml
Used by namespaces: qc_setpoint_units, setpoint_units
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| mW Setpoint | mW_setpoint |
- | - | Device setpoint expressed in milliwatts as configured in acquisition software. This is a commanded drive value, not a measured optical power; use metric_unit.mW for measured power values. |
| Percent | percent |
- | - | Setpoint expressed as a percentage of configured maximum or reference output. |
QC Type
Canonical source: vocab/qc_type.yaml
Used by namespaces: qc_type
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Alignment | alignment |
- | - | Optical alignment checks such as channel registration and beam path consistency. |
| Laser Power | laser_power |
- | - | Laser output and stability checks against expected setpoints. |
| Other | other |
- | - | QC activity that does not fit the predefined categories; document details in free text. |
| PSF | psf |
- | - | Point spread function measurements for optical performance and resolution tracking. |
| Stage | stage |
- | - | Mechanical stage repeatability and positioning accuracy checks. |
Maintenance
Maintenance Action
Canonical source: vocab/maintenance_action.yaml
Used by namespaces: action, maintenance_action
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Align | align |
realignment, alignment |
- | Adjusts optical or mechanical alignment to restore expected performance. Typical examples include beam path or stage alignment. |
| Calibrate | calibrate |
calibration |
- | Performs calibration against known references to ensure accurate measurement or positioning. Use for intensity, stage, focus, or scaling calibration events. |
| Clean | clean |
cleaning |
- | Removes contamination from optics, mechanics, or enclosures to recover performance and reliability. Includes routine cleaning of lenses and filters. |
| Install | install |
installation |
- | Installs a new component, module, or subsystem as part of maintenance or upgrade work. |
| Other | other |
misc |
- | Action does not match the predefined categories. Provide specific context in free-text details. |
| Repair | repair |
fix |
- | Fixes a confirmed fault or malfunction by restoring or replacing failing parts. Use when resolving a broken or degraded subsystem. |
| Replace | replace |
part replacement, part_replacement |
- | Substitutes a component with a new or refurbished part. Use when parts are worn, failed, or upgraded directly. |
| Service | service |
maintenance, calibration_and_alignment |
- | General maintenance intervention including inspection and routine adjustments. Used when work does not fit a more specific action type. |
| Update | update |
upgrade software, firmware update, software_update |
- | Applies software, firmware, or configuration updates to the instrument ecosystem. Use for non-hardware-change version or settings updates. |
Maintenance Reason
Canonical source: vocab/maintenance_reason.yaml
Used by namespaces: maintenance_reason, reason
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Check | check |
inspection, diagnostic |
- | Focused verification or inspection to confirm system status without major intervention. Includes diagnostic visits and quick health checks. |
| Cleaning | cleaning |
clean |
- | Triggered specifically by planned or corrective cleaning activity. |
| Install | install |
commissioning, setup |
- | Triggered by initial installation of a system, add-on module, or newly delivered component. Use for setup and commissioning events. |
| Issue Reported | issue_reported |
incident, fault reported |
- | Triggered by an issue report indicating degraded or failed operation, often before root cause is confirmed. |
| Other | other |
misc |
- | Reason does not fit the predefined categories. Document the rationale in free-text details. |
| Problem | problem |
issue, breakdown, repair |
- | Triggered by a reported fault, performance issue, or unexpected behavior. Use when intervention is reactive to a defect. |
| Scheduled | scheduled |
preventive maintenance, preventative_maintenance, preventive_maintenance, pm |
- | Planned preventive maintenance performed at regular intervals. Includes routine service contracts and periodic inspections. |
| Upgrade | upgrade |
enhancement |
- | Initiated to improve capability, performance, or feature set beyond baseline operation. Can include hardware or software enhancement projects. |
Maintenance Status
Canonical source: vocab/maintenance_status.yaml
Used by namespaces: maintenance_status
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| In Service | in_service |
operational, available, fully functional |
- | Instrument is fully available for use. |
| Limited Service | limited |
restricted, degraded, remaining issue |
- | Instrument is partially available with restrictions. |
| Out of Service | out_of_service |
offline, down |
- | Instrument is unavailable for use. |
Service Provider
Canonical source: vocab/service_provider.yaml
Used by namespaces: service_provider
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Distributor | distributor |
- | - | Service performed by an authorized distributor or reseller partner. |
| Internal | internal |
- | - | Service performed by in-house staff within the facility or institution. |
| Third Party | third_party |
- | - | Service performed by an independent external provider not classified as vendor or distributor. |
| Unknown | unknown |
- | - | Service provider type is not known or not documented. |
| Vendor | vendor |
- | - | Service performed directly by the original equipment manufacturer or official vendor organization. |
Record semantics
Identity Semantics
Canonical source: vocab/identity_semantics.yaml
Used by namespaces: identity_semantics
| Label | Canonical ID | Synonyms (rewrite-safe) | Classified values (preserved) | Description |
|---|---|---|---|---|
| Manufacturer | manufacturer |
- | - | Company/vendor/brand that makes the component or module. |
| Model | model |
- | - | Vendor-facing model name/designation; primary structured identity field. |
| Name | name |
- | - | Local/display/instance label only; not equivalent to model or product_code. Use only when it differs from model. |
| Product code | product_code |
- | - | Explicit catalogue/order/SKU/reference code only; optional and never inferred from model or name. |