Olympus BX60
About this microscope
Here you’ll find the hardware and capabilities currently recorded for this microscope. If you are planning an experiment, AIC Turku staff can help with access, training, and configuration choices.
- Widefield fluorescence: Widefield fluorescence
- Transmitted light: Transmitted brightfield, Phase contrast
Software
- Olympus Cell^D
Acquisition
Role: Acquisition
Version: Not documented
Hardware
Light sources
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Halogen Lamp Olympus 12V 100W halogen bulb
Olympus
Type: Halogen Lamp
Role: Transmitted Illumination
Power:100 W
Notes: Transmitted brightfield and phase contrast source. Broadband illumination. -
Arc Lamp Osram HBO 103W short arc bulb
Osram
Type: Arc Lamp
Power:103 W
Notes: Fluorescence excitation source. Broadband illumination.
Detectors
- Olympus DP71
Charge-Coupled Device
Type: Charge-Coupled Device
Pixel pitch:6.45 µm
Sensor format:1.45 MP
Notes: Manufacturer specifies 1.45 million effective sensor pixels; the physical array dimensions are not confirmed here. The documented live image format is 1360 x 1024 and pixel-shift output is 4080 x 3072; these output formats must not be used as physical sensor dimensions.
Objectives
View this microscope's objectives in the catalogue
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PlanC 4x/0.10 AIR
Olympus
Model: PlanC 4x/0.10
Magnification / NA:4x/0.1
Immersion: Air
Correction: Plan Achromat
Working distance:18.5 mm
AFC compatible: No
Installed: Yes -
UPlanFl 10x/0.30 Ph1 AIR
Olympus
Model: UPlanFl 10x/0.30 Ph1
Magnification / NA:10x/0.3
Immersion: Air
Correction: Plan Fluorite
Working distance:10.0 mm
AFC compatible: No
Installed: Yes
Specialties: phase -
UPlanFl 20x/0.50 Ph1 AIR
Olympus
Model: UPlanFl 20x/0.50 Ph1
Magnification / NA:20x/0.5
Immersion: Air
Correction: Plan Fluorite
Working distance:2.1 mm
AFC compatible: No
Installed: Yes
Specialties: phase -
UPlanFl 40x/0.75 Ph2 AIR
Olympus
Model: UPlanFl 40x/0.75 Ph2
Magnification / NA:40x/0.75
Immersion: Air
Correction: Plan Fluorite
Working distance:0.51 mm
AFC compatible: No
Installed: Yes
Specialties: phase -
UPlanApo 100x/1.35 Oil Iris Ph3 OIL
Olympus
Model: UPlanApo 100x/1.35 Oil Iris Ph3
Magnification / NA:100x/1.35
Immersion: Oil
Correction: Plan Apochromat
Working distance:0.17 mm
AFC compatible: No
Installed: Yes
Specialties: phase
Notes: Includes adjustable iris
- UPlanFl 60x/1.25 Oil Iris Ph3 OILNot installed
Olympus
Model: UPlanFl 60x/1.25 Oil Iris Ph3
Magnification / NA:60x/1.25
Immersion: Oil
Correction: Plan Fluorite
Working distance:0.12 mm
AFC compatible: No
Installed: No
Specialties: phase
Notes: Optional objective. Includes adjustable iris. Working distance cross-checked against an exact UPlanFl 60x/1.25 Oil Iris Ph3 listing.
Optical-path elements
- Fluorescence Turret
Cube
Element type: Turret
Pos 1: U-MWU (DAPI) (U-MWU) — Excitation: 355/50 nm. Dichroic Cutoff: 400 nm.
Pos 2: U-MWIB (GFP wide) (U-MWIB) — Excitation: 475/30 nm. Dichroic Cutoff: 505 nm.
Pos 3: U-MWIG (Alexa 546 / TRITC) (U-MWIG) — Excitation: 535/30 nm. Dichroic Cutoff: 565 nm.
Pos 4: U-MNIBA2 (GFP narrow) (U-MNIBA2) — emission 530/40 nm — Optional filter cube. Excitation: 480/20 nm. Dichroic Cutoff: 505 nm.
Latest QC measurements
Laser performance
No recent laser-performance measurements are recorded.
Optical resolution (PSF and alignment)
No recent PSF or chromatic-shift measurements are recorded for these objectives.
Objectives without recent measurements
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PlanC 4x/0.10 AIR (
4x_air) -
UPlanFl 10x/0.30 Ph1 AIR (
10x_air) -
UPlanFl 20x/0.50 Ph1 AIR (
20x_air) -
UPlanFl 40x/0.75 Ph2 AIR (
40x_air) -
UPlanApo 100x/1.35 Oil Iris Ph3 OIL (
100x_oil) -
UPlanFl 60x/1.25 Oil Iris Ph3 OIL (
60x_oil)
Information still to confirm
Some details in the facility record are incomplete. If any of these matter for your experiment, please check them with AIC Turku staff.
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Details needed for this configuration:
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Software version (
software[].version)