Test Scope X1

Work in progress

This dashboard is a work in progress. Please independently verify critical information, as not all features are working yet.

Current Status: 🟢 Online

Operational

📊 View QC & Maintenance History 🔬 Open Virtual Microscope

Manufacturer

Custom

Model

X1 Research Platform

Stand

Inverted

Eyepieces

Camera-only

Location

—

Purchased

2024

Funding

Internal development budget

Modalities

Widefield Fluorescence Point-Scanning Confocal Transmitted Brightfield

Modules

Hardware Autofocus Incubation Optogenetics

Software Environment

• MicroManager (v2.0)
  Acquisition
  Role: Acquisition
  Version: 2.0

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Hardware Configuration

🌡️ Environmental Control

• Environmental Control
  controlled temperature, controlled CO2
  Temperature control: Yes
  CO2 control: Yes
  Notes: Temperature and CO2 control enabled

💡 Light Sources

• 488 nm Laser Custom Photonics Sapphire 488
  Custom Photonics
  Type: Laser
  Role: Excitation
  Optical route: Confocal
  Wavelength: 488 nm
  Power: 50 mW
  Notes: Pulsed mode active

• multi-band LED CoolLED pE-300
  CoolLED
  Type: LED
  Role: Excitation
  Optical route: Epi
  Wavelength: multi-band
  Power: 25 mW
  Notes: Software alias: 'CoolLED_Main'

🧭 Scanner

• Galvanometric Scanner
  Type: Galvanometric Scanner
  Notes: Single-mirror galvo path for confocal test acquisitions.

📷 Detectors

• Hamamatsu ORCA-Flash4.0 V3
  Scientific CMOS
  Type: Scientific CMOS
  Pixel pitch: 6.5 µm
  QE peak: 82%
  Notes: Master detector

• Custom Photonics PMT-R928
  Photomultiplier Tube
  Type: Photomultiplier Tube
  Notes: Confocal channel A

🔬 Objectives

• UPlanSApo 20x/0.75 20x/0.75 AIR
  Olympus
  Model: UPlanSApo 20x/0.75
  Magnification / NA: 20x/0.75
  Immersion: Air
  Correction: Plan Apochromat
  Working distance: 0.60 mm
  Product code: OLY-20X-SA
  AFC compatible: Yes
  Installed: Yes
  Notes: General purpose testing

• UPlanSApo 60x/1.35 60x/1.35 OILAsk Staff to Install
  Olympus
  Model: UPlanSApo 60x/1.35
  Magnification / NA: 60x/1.35
  Immersion: Oil
  Correction: Plan Apochromat
  Working distance: 0.15 mm
  Product code: OLY-60X-SA
  AFC compatible: Yes
  Installed: No
  Notes: High-NA test

⚙️ Advanced Hardware

• Infrared Reflection Autofocus
  Installed: Yes
  Type: Infrared Reflection Autofocus

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QC Metrics: Latest Measurements

🔦 Laser Performance

• Laser Linearity 488nm (R²)

  ---

  0.997
  laser.488.linearity_r2

🔬 Optical Resolution (PSF & Alignment)

UPlanSApo 60x/1.35 60x/1.35 OIL (60x_oil)

• PSF XY Max FWHM (60x Oil, 525nm)

  ---

  240
  psf.60x_oil.525.fwhm_xy_max_nm

• PSF XY Min FWHM (60x Oil, 525nm)

  ---

  240
  psf.60x_oil.525.fwhm_xy_min_nm

• PSF Z FWHM (60x Oil, 525nm)

  ---

  600
  psf.60x_oil.525.fwhm_z_nm

• Chromatic Shift 561→488 (60x Oil, nm)

  ---

  60
  chromatic_shift.60x_oil.561_to_488.dist_nm

Objectives without recent data

• UPlanSApo 20x/0.75 20x/0.75 AIR (20x_air)

⚙️ Environment & Stage Performance

• Stage Repeatability σX (nm)

  ---

  200
  stage.repeatability_sigma_x_nm

• Stage Repeatability σY (nm)

  ---

  210
  stage.repeatability_sigma_y_nm

Metadata Completeness

• Missing required fields:

• instrument.location — Location

• Missing conditional fields (currently applicable):

• modules[].type — Module type

• modules[].manufacturer — Module manufacturer

• modules[].model — Module model

• hardware.detectors[].id — Detector ID

• hardware.detectors[].qe_peak_pct — Detector peak quantum efficiency (%)

• Legacy aliases in use (fallback accepted):

• modules[].name → modules[].type

• hardware.detectors[].pixel_size_um → hardware.detectors[].pixel_pitch_um

• hardware.detectors[].min_nm → hardware.detectors[].collection_min_nm

• hardware.detectors[].max_nm → hardware.detectors[].collection_max_nm

• hardware.detectors[].channel_center_nm → hardware.detectors[].collection_center_nm

• hardware.detectors[].bandwidth_nm → hardware.detectors[].collection_width_nm